The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae
The availability of next-generation sequencing (NGS) in recent years has facilitated a revolution in the availability of mitochondrial (mt) genome sequences. The mt genome is a powerful tool for comparative studies and resolving the phylogenetic relationships among insect lineages. The mt genomes of...
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MDPI AG
2021-11-01
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| Series: | Insects |
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| author | Sam Pedro Galilee Ayivi Yao Tong Kenneth B. Storey Dan-Na Yu Jia-Yong Zhang |
| author_facet | Sam Pedro Galilee Ayivi Yao Tong Kenneth B. Storey Dan-Na Yu Jia-Yong Zhang |
| author_sort | Sam Pedro Galilee Ayivi |
| collection | DOAJ |
| description | The availability of next-generation sequencing (NGS) in recent years has facilitated a revolution in the availability of mitochondrial (mt) genome sequences. The mt genome is a powerful tool for comparative studies and resolving the phylogenetic relationships among insect lineages. The mt genomes of phytophagous scarabs of the subfamilies Cetoniinae and Dynastinae were under-represented in GenBank. Previous research found that the subfamily Rutelinae was recovered as a paraphyletic group because the few representatives of the subfamily Dynastinae clustered into Rutelinae, but the subfamily position of Dynastinae was still unclear. In the present study, we sequenced 18 mt genomes from Dynastinae and Cetoniinae using next-generation sequencing (NGS) to re-assess the phylogenetic relationships within Scarabaeidae. All sequenced mt genomes contained 37 sets of genes (13 protein-coding genes, 22 tRNAs, and two ribosomal RNAs), with one long control region, but the gene order was not the same between Cetoniinae and Dynastinae species. All mt genomes of Dynastinae species showed the same gene rearrangement of trnQ-NCR-trnI-trnM, whereas all mt genomes of Cetoniinae species showed the ancestral insect gene order of trnI-trnQ-trnM. Phylogenetic analyses (IQ-tree and MrBayes) were conducted using 13 protein-coding genes based on nucleotide and amino acid datasets. In the ML and BI trees, we recovered the monophyly of Rutelinae, Cetoniinae, Dynastinae, and Sericinae, and the non-monophyly of Melolonthinae. Cetoniinae was shown to be a sister clade to (Dynastinae + Rutelinae). |
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| last_indexed | 2024-03-10T05:24:19Z |
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| spelling | doaj.art-129096bc9a4d41d88e1360836d1f034d2023-11-22T23:47:21ZengMDPI AGInsects2075-44502021-11-011211102510.3390/insects12111025The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within ScarabaeidaeSam Pedro Galilee Ayivi0Yao Tong1Kenneth B. Storey2Dan-Na Yu3Jia-Yong Zhang4Department of Biology, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, ChinaDepartment of Biology, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, ChinaDepartment of Biology, Carleton University, Ottawa, ON K1S5B6, CanadaDepartment of Biology, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, ChinaDepartment of Biology, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, ChinaThe availability of next-generation sequencing (NGS) in recent years has facilitated a revolution in the availability of mitochondrial (mt) genome sequences. The mt genome is a powerful tool for comparative studies and resolving the phylogenetic relationships among insect lineages. The mt genomes of phytophagous scarabs of the subfamilies Cetoniinae and Dynastinae were under-represented in GenBank. Previous research found that the subfamily Rutelinae was recovered as a paraphyletic group because the few representatives of the subfamily Dynastinae clustered into Rutelinae, but the subfamily position of Dynastinae was still unclear. In the present study, we sequenced 18 mt genomes from Dynastinae and Cetoniinae using next-generation sequencing (NGS) to re-assess the phylogenetic relationships within Scarabaeidae. All sequenced mt genomes contained 37 sets of genes (13 protein-coding genes, 22 tRNAs, and two ribosomal RNAs), with one long control region, but the gene order was not the same between Cetoniinae and Dynastinae species. All mt genomes of Dynastinae species showed the same gene rearrangement of trnQ-NCR-trnI-trnM, whereas all mt genomes of Cetoniinae species showed the ancestral insect gene order of trnI-trnQ-trnM. Phylogenetic analyses (IQ-tree and MrBayes) were conducted using 13 protein-coding genes based on nucleotide and amino acid datasets. In the ML and BI trees, we recovered the monophyly of Rutelinae, Cetoniinae, Dynastinae, and Sericinae, and the non-monophyly of Melolonthinae. Cetoniinae was shown to be a sister clade to (Dynastinae + Rutelinae).https://www.mdpi.com/2075-4450/12/11/1025Scarabaeidaephytophagousmitochondrial genomegene rearrangementnon-coding region (NCR)phylogeny |
| spellingShingle | Sam Pedro Galilee Ayivi Yao Tong Kenneth B. Storey Dan-Na Yu Jia-Yong Zhang The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae Insects Scarabaeidae phytophagous mitochondrial genome gene rearrangement non-coding region (NCR) phylogeny |
| title | The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae |
| title_full | The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae |
| title_fullStr | The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae |
| title_full_unstemmed | The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae |
| title_short | The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae |
| title_sort | mitochondrial genomes of 18 new pleurosticti coleoptera scarabaeidae exhibit a novel trnq ncr trni trnm gene rearrangement and clarify phylogenetic relationships of subfamilies within scarabaeidae |
| topic | Scarabaeidae phytophagous mitochondrial genome gene rearrangement non-coding region (NCR) phylogeny |
| url | https://www.mdpi.com/2075-4450/12/11/1025 |
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