Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations
Background: We previously reported that interstitial injection of bleomycin (BLM) reduces the size of early-stage extracranial arteriovenous malformation (AVM). Here, we sought to investigate the potential mechanism of BLM in treating extracranial AVM. Methods: Samples of human extracranial AVM (n=3...
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KeAi Communications Co. Ltd.
2021-12-01
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| Series: | Chinese Journal of Plastic and Reconstructive Surgery |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2096691121001102 |
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| author | Congzhen Qiao Yajing Qiu Yun Zou Chen Hua Yuanbo Li Yunbo Jin Xiaoxi Lin |
| author_facet | Congzhen Qiao Yajing Qiu Yun Zou Chen Hua Yuanbo Li Yunbo Jin Xiaoxi Lin |
| author_sort | Congzhen Qiao |
| collection | DOAJ |
| description | Background: We previously reported that interstitial injection of bleomycin (BLM) reduces the size of early-stage extracranial arteriovenous malformation (AVM). Here, we sought to investigate the potential mechanism of BLM in treating extracranial AVM. Methods: Samples of human extracranial AVM (n=3) with no pharmacological treatment were harvested. AVM endothelial cells were isolated and cultured in primary cell culture. The transcriptome was examined using RNA-sequencing, and differentially expressed C-type lectin domain family 14 member A (CLEC14A) was validated at the transcriptomic and protein levels. Immunocytochemical staining of CLEC14A was performed in samples of human extracranial AVM, with and without BLM treatment. Results: Through second-generation sequencing, we found that the expression of 5 689 genes were differentially increased or decreased following 24-h BLM stimulation. We found that CLEC14A may play an important role in the progression of AVM and can be inhibited by BLM treatment. Conclusion: BLM inhibited CLEC14A expression to attenuate the progression of AVM. |
| first_indexed | 2024-04-13T04:54:01Z |
| format | Article |
| id | doaj.art-1293412168b641168fcaf2c5c90de2a6 |
| institution | Directory Open Access Journal |
| issn | 2096-6911 |
| language | English |
| last_indexed | 2024-04-13T04:54:01Z |
| publishDate | 2021-12-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Chinese Journal of Plastic and Reconstructive Surgery |
| spelling | doaj.art-1293412168b641168fcaf2c5c90de2a62022-12-22T03:01:34ZengKeAi Communications Co. Ltd.Chinese Journal of Plastic and Reconstructive Surgery2096-69112021-12-0134175180Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformationsCongzhen Qiao0Yajing Qiu1Yun Zou2Chen Hua3Yuanbo Li4Yunbo Jin5Xiaoxi Lin6Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaCorresponding authors. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Huangpu District, Shanghai 200011, China.; Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaCorresponding authors. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Huangpu District, Shanghai 200011, China.; Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaBackground: We previously reported that interstitial injection of bleomycin (BLM) reduces the size of early-stage extracranial arteriovenous malformation (AVM). Here, we sought to investigate the potential mechanism of BLM in treating extracranial AVM. Methods: Samples of human extracranial AVM (n=3) with no pharmacological treatment were harvested. AVM endothelial cells were isolated and cultured in primary cell culture. The transcriptome was examined using RNA-sequencing, and differentially expressed C-type lectin domain family 14 member A (CLEC14A) was validated at the transcriptomic and protein levels. Immunocytochemical staining of CLEC14A was performed in samples of human extracranial AVM, with and without BLM treatment. Results: Through second-generation sequencing, we found that the expression of 5 689 genes were differentially increased or decreased following 24-h BLM stimulation. We found that CLEC14A may play an important role in the progression of AVM and can be inhibited by BLM treatment. Conclusion: BLM inhibited CLEC14A expression to attenuate the progression of AVM.http://www.sciencedirect.com/science/article/pii/S2096691121001102BleomycinExtracranial arteriovenous malformationCLEC14ARNA-Sequencing |
| spellingShingle | Congzhen Qiao Yajing Qiu Yun Zou Chen Hua Yuanbo Li Yunbo Jin Xiaoxi Lin Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations Chinese Journal of Plastic and Reconstructive Surgery Bleomycin Extracranial arteriovenous malformation CLEC14A RNA-Sequencing |
| title | Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations |
| title_full | Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations |
| title_fullStr | Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations |
| title_full_unstemmed | Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations |
| title_short | Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations |
| title_sort | bleomycin inhibits clec14a to attenuate the progression of extracranial arteriovenous malformations |
| topic | Bleomycin Extracranial arteriovenous malformation CLEC14A RNA-Sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2096691121001102 |
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