Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation
Mayaro virus (MAYV) is an emerging arthritogenic alphavirus belonging to the Togaviridae family. Infection leads to a dengue-like illness accompanied by severe polyarthralgia. However, the molecular and cellular mechanisms of arthritis as a result of MAYV infection remain poorly understood. In the p...
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MDPI AG
2019-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/9/797 |
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| author | Michèle Bengue Pauline Ferraris Cécile Baronti Cheikh Tidiane Diagne Loïc Talignani Sineewanlaya Wichit Florian Liegeois Catherine Bisbal Antoine Nougairède Dorothée Missé |
| author_facet | Michèle Bengue Pauline Ferraris Cécile Baronti Cheikh Tidiane Diagne Loïc Talignani Sineewanlaya Wichit Florian Liegeois Catherine Bisbal Antoine Nougairède Dorothée Missé |
| author_sort | Michèle Bengue |
| collection | DOAJ |
| description | Mayaro virus (MAYV) is an emerging arthritogenic alphavirus belonging to the Togaviridae family. Infection leads to a dengue-like illness accompanied by severe polyarthralgia. However, the molecular and cellular mechanisms of arthritis as a result of MAYV infection remain poorly understood. In the present study, we assess the susceptibility of human chondrocytes (HC), fibroblast-like synoviocytes and osteoblasts that are the major cell types involved in osteoarthritis, to infection with MAYV. We show that these cells are highly permissive to MAYV infection and that viral RNA copy number and viral titers increase over time in infected cells. Knowing that HC are the primary cells in articular cartilage and are essential for maintaining the cartilaginous matrix, gene expression studies were conducted in MAYV-infected primary HC using polymerase chain reaction (PCR) arrays. The infection of the latter cells resulted in an induction in the expression of several matrix metalloproteinases (MMP) including MMP1, MMP7, MMP8, MMP10, MMP13, MMP14 and MMP15 which could be involved in the destruction of articular cartilage. Infected HC were also found to express significantly increased levels of various IFN-stimulated genes and arthritogenic mediators such as TNF-α and IL-6. In conclusion, MAYV-infected primary HC overexpress arthritis-related genes, which may contribute to joint degradation and pathogenesis. |
| first_indexed | 2024-12-12T22:02:35Z |
| format | Article |
| id | doaj.art-129433c9c506418895673d2037ca7da2 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-12T22:02:35Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-129433c9c506418895673d2037ca7da22022-12-22T00:10:28ZengMDPI AGViruses1999-49152019-08-0111979710.3390/v11090797v11090797Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint DegradationMichèle Bengue0Pauline Ferraris1Cécile Baronti2Cheikh Tidiane Diagne3Loïc Talignani4Sineewanlaya Wichit5Florian Liegeois6Catherine Bisbal7Antoine Nougairède8Dorothée Missé9MIVEGEC, IRD, Univ. Montpellier, CNRS, 34394 Montpellier, FranceMIVEGEC, IRD, Univ. Montpellier, CNRS, 34394 Montpellier, FranceUnité des virus émergents, Aix Marseille Univ-IRD 190, Inserm 1207-IHU Méditerranée Infection, 13385 Marseille, FranceMIVEGEC, IRD, Univ. Montpellier, CNRS, 34394 Montpellier, FranceMIVEGEC, IRD, Univ. Montpellier, CNRS, 34394 Montpellier, FranceDepartment of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, ThailandMIVEGEC, IRD, Univ. Montpellier, CNRS, 34394 Montpellier, FrancePhyMedExp, CNRS UMR 9214, INSERM U1046, University of Montpellier, 34295 Montpellier, FranceUnité des virus émergents, Aix Marseille Univ-IRD 190, Inserm 1207-IHU Méditerranée Infection, 13385 Marseille, FranceMIVEGEC, IRD, Univ. Montpellier, CNRS, 34394 Montpellier, FranceMayaro virus (MAYV) is an emerging arthritogenic alphavirus belonging to the Togaviridae family. Infection leads to a dengue-like illness accompanied by severe polyarthralgia. However, the molecular and cellular mechanisms of arthritis as a result of MAYV infection remain poorly understood. In the present study, we assess the susceptibility of human chondrocytes (HC), fibroblast-like synoviocytes and osteoblasts that are the major cell types involved in osteoarthritis, to infection with MAYV. We show that these cells are highly permissive to MAYV infection and that viral RNA copy number and viral titers increase over time in infected cells. Knowing that HC are the primary cells in articular cartilage and are essential for maintaining the cartilaginous matrix, gene expression studies were conducted in MAYV-infected primary HC using polymerase chain reaction (PCR) arrays. The infection of the latter cells resulted in an induction in the expression of several matrix metalloproteinases (MMP) including MMP1, MMP7, MMP8, MMP10, MMP13, MMP14 and MMP15 which could be involved in the destruction of articular cartilage. Infected HC were also found to express significantly increased levels of various IFN-stimulated genes and arthritogenic mediators such as TNF-α and IL-6. In conclusion, MAYV-infected primary HC overexpress arthritis-related genes, which may contribute to joint degradation and pathogenesis.https://www.mdpi.com/1999-4915/11/9/797arbovirusMayaro virusmatrix metalloproteinasesextracellular matrixchondrocytesynoviocyteosteoblastinnate immunityIL-6TNF-α |
| spellingShingle | Michèle Bengue Pauline Ferraris Cécile Baronti Cheikh Tidiane Diagne Loïc Talignani Sineewanlaya Wichit Florian Liegeois Catherine Bisbal Antoine Nougairède Dorothée Missé Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation Viruses arbovirus Mayaro virus matrix metalloproteinases extracellular matrix chondrocyte synoviocyte osteoblast innate immunity IL-6 TNF-α |
| title | Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation |
| title_full | Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation |
| title_fullStr | Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation |
| title_full_unstemmed | Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation |
| title_short | Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation |
| title_sort | mayaro virus infects human chondrocytes and induces the expression of arthritis related genes associated with joint degradation |
| topic | arbovirus Mayaro virus matrix metalloproteinases extracellular matrix chondrocyte synoviocyte osteoblast innate immunity IL-6 TNF-α |
| url | https://www.mdpi.com/1999-4915/11/9/797 |
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