Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion
Three potential rhizobacteria namely Burkholderia gladioli (MTCC 10216), Pseudomonas sp. (MTCC 9002) and Bacillus subtilis (MTCC 8528) procured from IMTECH, Chandigarh (India) were evaluated individually and as consortia for its phosphate (P) solubilizing ability and effect of growth of fenugreek (T...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-12-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020325445 |
| _version_ | 1818944257692532736 |
|---|---|
| author | Pankaj Kumar Abhinav Aeron Niru Shaw Ajay Singh V.K. Bajpai Shailja Pant Ramesh Chandra Dubey |
| author_facet | Pankaj Kumar Abhinav Aeron Niru Shaw Ajay Singh V.K. Bajpai Shailja Pant Ramesh Chandra Dubey |
| author_sort | Pankaj Kumar |
| collection | DOAJ |
| description | Three potential rhizobacteria namely Burkholderia gladioli (MTCC 10216), Pseudomonas sp. (MTCC 9002) and Bacillus subtilis (MTCC 8528) procured from IMTECH, Chandigarh (India) were evaluated individually and as consortia for its phosphate (P) solubilizing ability and effect of growth of fenugreek (Trigonella foenum-graecum L.) and tomato (Lycopersicon esculentum L.). Phosphate solubilizing ability of these strains individually and as consortia was tested on Pikovskayas agar medium, Phosphate solubilizing agar medium and National Botanical Research Institute phosphate agar medium containing six different sources of insoluble inorganic phosphate such as tri-calcium phosphate (TCP), di-calcium phosphate (DCP), zinc phosphate (ZP), ferric phosphate (FP), sodium di-hydrogen phosphate (SP), and aluminum phosphate (AP), and two organic P such as calcium and sodium phytate. The maximum P solubilizing ability was recorded in consortium-4 having all three potential bacterial strains. Phosphate solubilization after 7th day of incubation was 37.9 mg/100 ml of TCP, 40.01 mg/100 ml of DCP, 15.79 mg/100 ml of FP, 43.02 mg/100 ml of SP, no solubilization of ZP and AP, 39.75 mg/100 ml of calcium phytate and 24.01mg/100 ml of sodium phytate. Seed germination and the other plant parameters such as plant height and weight significantly increased in fenugreek and tomato seeds, bio-primed with consortium-4 followed by consortium-3. After bio-priming of seeds in pot assay, the level of phosphorus in soil got increased by 54% in consortium-4 treated soil followed by consortium-3 (47%) over untreated control soil. Based on these findings, consoritium-4 could be recommended as a good bio-inoculant for fenugreek, tomato and other crops in comparison to individual strains and other consortia. |
| first_indexed | 2024-12-20T07:40:21Z |
| format | Article |
| id | doaj.art-12947fac9cd74c5c935f9b917aedd453 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-20T07:40:21Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-12947fac9cd74c5c935f9b917aedd4532022-12-21T19:48:08ZengElsevierHeliyon2405-84402020-12-01612e05701Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotionPankaj Kumar0Abhinav Aeron1Niru Shaw2Ajay Singh3V.K. Bajpai4Shailja Pant5Ramesh Chandra Dubey6Department of Microbiology, Dolphin (PG) College of Science and Agriculture, Chunni Kalan, Fatehgarh Sahib, Chandigarh, 140307, Punjab, India; Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, 248007, Uttarakhand, India; Corresponding author.Department of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar 249404, Uttarakhand, IndiaDepartment of Microbiology, Dolphin (PG) College of Science and Agriculture, Chunni Kalan, Fatehgarh Sahib, Chandigarh, 140307, Punjab, IndiaDepartment of Food Technology, Mata Gujri College, Fatehgarh Sahib, 140406, Punjab, IndiaDepartment of Energy and Materials Engineering, Dongguk University-Seoul, 30 Pildong-ro 1-gil, Seoul 04620, Republic of KoreaDepartment of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, 248007, Uttarakhand, IndiaDepartment of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar 249404, Uttarakhand, IndiaThree potential rhizobacteria namely Burkholderia gladioli (MTCC 10216), Pseudomonas sp. (MTCC 9002) and Bacillus subtilis (MTCC 8528) procured from IMTECH, Chandigarh (India) were evaluated individually and as consortia for its phosphate (P) solubilizing ability and effect of growth of fenugreek (Trigonella foenum-graecum L.) and tomato (Lycopersicon esculentum L.). Phosphate solubilizing ability of these strains individually and as consortia was tested on Pikovskayas agar medium, Phosphate solubilizing agar medium and National Botanical Research Institute phosphate agar medium containing six different sources of insoluble inorganic phosphate such as tri-calcium phosphate (TCP), di-calcium phosphate (DCP), zinc phosphate (ZP), ferric phosphate (FP), sodium di-hydrogen phosphate (SP), and aluminum phosphate (AP), and two organic P such as calcium and sodium phytate. The maximum P solubilizing ability was recorded in consortium-4 having all three potential bacterial strains. Phosphate solubilization after 7th day of incubation was 37.9 mg/100 ml of TCP, 40.01 mg/100 ml of DCP, 15.79 mg/100 ml of FP, 43.02 mg/100 ml of SP, no solubilization of ZP and AP, 39.75 mg/100 ml of calcium phytate and 24.01mg/100 ml of sodium phytate. Seed germination and the other plant parameters such as plant height and weight significantly increased in fenugreek and tomato seeds, bio-primed with consortium-4 followed by consortium-3. After bio-priming of seeds in pot assay, the level of phosphorus in soil got increased by 54% in consortium-4 treated soil followed by consortium-3 (47%) over untreated control soil. Based on these findings, consoritium-4 could be recommended as a good bio-inoculant for fenugreek, tomato and other crops in comparison to individual strains and other consortia.http://www.sciencedirect.com/science/article/pii/S2405844020325445BioinformaticsBiotechnologyEcologyMicrobiologyPlant biologyAgricultural soil science |
| spellingShingle | Pankaj Kumar Abhinav Aeron Niru Shaw Ajay Singh V.K. Bajpai Shailja Pant Ramesh Chandra Dubey Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion Heliyon Bioinformatics Biotechnology Ecology Microbiology Plant biology Agricultural soil science |
| title | Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion |
| title_full | Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion |
| title_fullStr | Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion |
| title_full_unstemmed | Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion |
| title_short | Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion |
| title_sort | seed bio priming with tri species consortia of phosphate solubilizing rhizobacteria psr and its effect on plant growth promotion |
| topic | Bioinformatics Biotechnology Ecology Microbiology Plant biology Agricultural soil science |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020325445 |
| work_keys_str_mv | AT pankajkumar seedbioprimingwithtrispeciesconsortiaofphosphatesolubilizingrhizobacteriapsranditseffectonplantgrowthpromotion AT abhinavaeron seedbioprimingwithtrispeciesconsortiaofphosphatesolubilizingrhizobacteriapsranditseffectonplantgrowthpromotion AT nirushaw seedbioprimingwithtrispeciesconsortiaofphosphatesolubilizingrhizobacteriapsranditseffectonplantgrowthpromotion AT ajaysingh seedbioprimingwithtrispeciesconsortiaofphosphatesolubilizingrhizobacteriapsranditseffectonplantgrowthpromotion AT vkbajpai seedbioprimingwithtrispeciesconsortiaofphosphatesolubilizingrhizobacteriapsranditseffectonplantgrowthpromotion AT shailjapant seedbioprimingwithtrispeciesconsortiaofphosphatesolubilizingrhizobacteriapsranditseffectonplantgrowthpromotion AT rameshchandradubey seedbioprimingwithtrispeciesconsortiaofphosphatesolubilizingrhizobacteriapsranditseffectonplantgrowthpromotion |