ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging
Photoactivatable fluorophores have become an important technique for the high spatiotemporal resolution of biological imaging. Here, we developed a novel photoactivatable probe (PHBT), which is based on 2-(2-hydroxyphenyl)benzothiazole (HBT), a small organic fluorophore known for its classic lumines...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-10-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/16/10/1684 |
| _version_ | 1798035293844013056 |
|---|---|
| author | Xiaohong Zhou Yuren Jiang Xiongjie Zhao Dong Guo |
| author_facet | Xiaohong Zhou Yuren Jiang Xiongjie Zhao Dong Guo |
| author_sort | Xiaohong Zhou |
| collection | DOAJ |
| description | Photoactivatable fluorophores have become an important technique for the high spatiotemporal resolution of biological imaging. Here, we developed a novel photoactivatable probe (PHBT), which is based on 2-(2-hydroxyphenyl)benzothiazole (HBT), a small organic fluorophore known for its classic luminescence mechanism through excited-state intramolecular proton transfer (ESIPT) with the keto form and the enol form. After photocleavage, PHBT released a ratiometric fluorophore HBT, which showed dual emission bands with more than 73-fold fluorescence enhancement at 512 nm in buffer and more than 69-fold enhancement at 452 nm in bovine serum. The probe displayed a high ratiometric imaging resolution and is believed to have a wide application in biological imaging. |
| first_indexed | 2024-04-11T20:55:18Z |
| format | Article |
| id | doaj.art-1294e3b0f97c402c8d8d2098999678a2 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T20:55:18Z |
| publishDate | 2016-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-1294e3b0f97c402c8d8d2098999678a22022-12-22T04:03:41ZengMDPI AGSensors1424-82202016-10-011610168410.3390/s16101684s16101684ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal BioimagingXiaohong Zhou0Yuren Jiang1Xiongjie Zhao2Dong Guo3College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaPhotoactivatable fluorophores have become an important technique for the high spatiotemporal resolution of biological imaging. Here, we developed a novel photoactivatable probe (PHBT), which is based on 2-(2-hydroxyphenyl)benzothiazole (HBT), a small organic fluorophore known for its classic luminescence mechanism through excited-state intramolecular proton transfer (ESIPT) with the keto form and the enol form. After photocleavage, PHBT released a ratiometric fluorophore HBT, which showed dual emission bands with more than 73-fold fluorescence enhancement at 512 nm in buffer and more than 69-fold enhancement at 452 nm in bovine serum. The probe displayed a high ratiometric imaging resolution and is believed to have a wide application in biological imaging.http://www.mdpi.com/1424-8220/16/10/1684ESIPTphotoactivatableratiometric |
| spellingShingle | Xiaohong Zhou Yuren Jiang Xiongjie Zhao Dong Guo ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging Sensors ESIPT photoactivatable ratiometric |
| title | ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging |
| title_full | ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging |
| title_fullStr | ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging |
| title_full_unstemmed | ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging |
| title_short | ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging |
| title_sort | esipt based photoactivatable fluorescent probe for ratiometric spatiotemporal bioimaging |
| topic | ESIPT photoactivatable ratiometric |
| url | http://www.mdpi.com/1424-8220/16/10/1684 |
| work_keys_str_mv | AT xiaohongzhou esiptbasedphotoactivatablefluorescentprobeforratiometricspatiotemporalbioimaging AT yurenjiang esiptbasedphotoactivatablefluorescentprobeforratiometricspatiotemporalbioimaging AT xiongjiezhao esiptbasedphotoactivatablefluorescentprobeforratiometricspatiotemporalbioimaging AT dongguo esiptbasedphotoactivatablefluorescentprobeforratiometricspatiotemporalbioimaging |