Histological analysis of the medial gastrocnemius muscle in young healthy children
Introduction: Histological data on muscle fiber size and proportion in (very) young typically developing (TD) children is not well documented and data on capillarization and satellite cell content are also lacking.Aims: This study investigated the microscopic properties of the medial gastrocnemius m...
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2024.1336283/full |
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| author | Anke Andries Jorieke Deschrevel Karen Maes Nathalie De Beukelaer Marlies Corvelyn Lauraine Staut Hannah De Houwer Domiziana Costamagna Domiziana Costamagna Domiziana Costamagna Stefaan Nijs Willem-Jan Metsemakers Elga Nijs Greet Hens Eva De Wachter Sandra Prinsen Kaat Desloovere Anja Van Campenhout Ghislaine Gayan-Ramirez |
| author_facet | Anke Andries Jorieke Deschrevel Karen Maes Nathalie De Beukelaer Marlies Corvelyn Lauraine Staut Hannah De Houwer Domiziana Costamagna Domiziana Costamagna Domiziana Costamagna Stefaan Nijs Willem-Jan Metsemakers Elga Nijs Greet Hens Eva De Wachter Sandra Prinsen Kaat Desloovere Anja Van Campenhout Ghislaine Gayan-Ramirez |
| author_sort | Anke Andries |
| collection | DOAJ |
| description | Introduction: Histological data on muscle fiber size and proportion in (very) young typically developing (TD) children is not well documented and data on capillarization and satellite cell content are also lacking.Aims: This study investigated the microscopic properties of the medial gastrocnemius muscle in growing TD children, grouped according to age and gender to provide normal reference values in healthy children.Methods: Microbiopsies of the medial gastrocnemius (MG) muscle were collected in 46 TD boys and girls aged 2–10 years subdivided into 4 age groups (2–4, 4–6, 6–8 and 8–10 years). Sections were immunostained to assess fiber type cross-sectional area (fCSA) and proportion, the number of satellite cells (SC), capillary to fiber ratio (C/F), capillary density for type I and II fiber (CFD), capillary domain, capillary-to-fiber perimeter exchange index (CFPE) and heterogeneity index. fCSA was normalized to fibula length2 and the coefficient of variation (CV) was calculated to reflect fCSA intrasubject variability.Results: Absolute fCSA of all fibers increased with age (r = 0.72, p < 0.001) but more in boys (+112%, p < 0.05) than in girls (+48%, p > 0.05) Normalized fCSA, CV and fiber proportion did not differ between age groups and gender. C/F was strongly correlated with age in boys (r = 0.83, p < 0.001), and to a lesser extent in girls (r = 0.37, p = 0.115), while other capillary parameters as well as the number of SC remained stable with increasing age in boys and girls.Discussion: This study provides reference values of histological measures in MG according to age in normally growing boys and girls. These data may be used as a reference to determine disease impact and efficacy of therapeutic approach on the muscle. |
| first_indexed | 2024-04-24T12:02:37Z |
| format | Article |
| id | doaj.art-1295714ad5444f5990249649b4737c19 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-24T12:02:37Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-1295714ad5444f5990249649b4737c192024-04-08T12:40:24ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2024-04-011510.3389/fphys.2024.13362831336283Histological analysis of the medial gastrocnemius muscle in young healthy childrenAnke Andries0Jorieke Deschrevel1Karen Maes2Nathalie De Beukelaer3Marlies Corvelyn4Lauraine Staut5Hannah De Houwer6Domiziana Costamagna7Domiziana Costamagna8Domiziana Costamagna9Stefaan Nijs10Willem-Jan Metsemakers11Elga Nijs12Greet Hens13Eva De Wachter14Sandra Prinsen15Kaat Desloovere16Anja Van Campenhout17Ghislaine Gayan-Ramirez18Laboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Diseases and Metabolism, KU-Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Diseases and Metabolism, KU-Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Diseases and Metabolism, KU-Leuven, Leuven, BelgiumNeurorehabilitation Group, Department of Rehabilitation Sciences, KU-Leuven, Leuven, BelgiumStem Cell and Developmental Biology, Department of Development and Regeneration, KU-Leuven, Leuven, BelgiumNeurorehabilitation Group, Department of Rehabilitation Sciences, KU-Leuven, Leuven, BelgiumPediatric Orthopedics, Department of Development and Regeneration, KU-Leuven, Leuven, BelgiumNeurorehabilitation Group, Department of Rehabilitation Sciences, KU-Leuven, Leuven, BelgiumStem Cell and Developmental Biology, Department of Development and Regeneration, KU-Leuven, Leuven, BelgiumExercise Physiology Research Group, Department of Movement Sciences, KU-Leuven, Leuven, BelgiumDepartment of Trauma Surgery, University Hospitals Leuven, Leuven, BelgiumDepartment of Trauma Surgery, University Hospitals Leuven, Leuven, BelgiumDepartment of Trauma Surgery, University Hospitals Leuven, Leuven, BelgiumDepartment of Ear Nose Throat, University Hospitals Leuven, Leuven, BelgiumDepartment of Orthopaedic Surgery, University Hospitals Leuven, Leuven, BelgiumDepartment of Orthopaedic Surgery, University Hospitals Leuven, Leuven, BelgiumNeurorehabilitation Group, Department of Rehabilitation Sciences, KU-Leuven, Leuven, BelgiumPediatric Orthopedics, Department of Development and Regeneration, KU-Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Diseases and Metabolism, KU-Leuven, Leuven, BelgiumIntroduction: Histological data on muscle fiber size and proportion in (very) young typically developing (TD) children is not well documented and data on capillarization and satellite cell content are also lacking.Aims: This study investigated the microscopic properties of the medial gastrocnemius muscle in growing TD children, grouped according to age and gender to provide normal reference values in healthy children.Methods: Microbiopsies of the medial gastrocnemius (MG) muscle were collected in 46 TD boys and girls aged 2–10 years subdivided into 4 age groups (2–4, 4–6, 6–8 and 8–10 years). Sections were immunostained to assess fiber type cross-sectional area (fCSA) and proportion, the number of satellite cells (SC), capillary to fiber ratio (C/F), capillary density for type I and II fiber (CFD), capillary domain, capillary-to-fiber perimeter exchange index (CFPE) and heterogeneity index. fCSA was normalized to fibula length2 and the coefficient of variation (CV) was calculated to reflect fCSA intrasubject variability.Results: Absolute fCSA of all fibers increased with age (r = 0.72, p < 0.001) but more in boys (+112%, p < 0.05) than in girls (+48%, p > 0.05) Normalized fCSA, CV and fiber proportion did not differ between age groups and gender. C/F was strongly correlated with age in boys (r = 0.83, p < 0.001), and to a lesser extent in girls (r = 0.37, p = 0.115), while other capillary parameters as well as the number of SC remained stable with increasing age in boys and girls.Discussion: This study provides reference values of histological measures in MG according to age in normally growing boys and girls. These data may be used as a reference to determine disease impact and efficacy of therapeutic approach on the muscle.https://www.frontiersin.org/articles/10.3389/fphys.2024.1336283/fullboysgirlsmuscle capillariesmuscle fiber size and proportionmyosin heavy chainsatellite cells |
| spellingShingle | Anke Andries Jorieke Deschrevel Karen Maes Nathalie De Beukelaer Marlies Corvelyn Lauraine Staut Hannah De Houwer Domiziana Costamagna Domiziana Costamagna Domiziana Costamagna Stefaan Nijs Willem-Jan Metsemakers Elga Nijs Greet Hens Eva De Wachter Sandra Prinsen Kaat Desloovere Anja Van Campenhout Ghislaine Gayan-Ramirez Histological analysis of the medial gastrocnemius muscle in young healthy children Frontiers in Physiology boys girls muscle capillaries muscle fiber size and proportion myosin heavy chain satellite cells |
| title | Histological analysis of the medial gastrocnemius muscle in young healthy children |
| title_full | Histological analysis of the medial gastrocnemius muscle in young healthy children |
| title_fullStr | Histological analysis of the medial gastrocnemius muscle in young healthy children |
| title_full_unstemmed | Histological analysis of the medial gastrocnemius muscle in young healthy children |
| title_short | Histological analysis of the medial gastrocnemius muscle in young healthy children |
| title_sort | histological analysis of the medial gastrocnemius muscle in young healthy children |
| topic | boys girls muscle capillaries muscle fiber size and proportion myosin heavy chain satellite cells |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2024.1336283/full |
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