Antifungal Activity and Mechanism of Electron Beam Irradiation Against Rhizopus oryzae
Electron beam irradiation is a physical fungicidal technique that has emerged as a potential application in China. However, its antifungal activity and mechanism against Rhizopus oryzae have not been reported. Thus, this study aimed to investigate the antifungal activity and mechanism of electron be...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X2306742X |
| _version_ | 1797788437876572160 |
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| author | Lili Li Linlin Fan Feifei Shang Yuqiao Zhang Liang Shuai Yuhua xie Zhenhua Duan |
| author_facet | Lili Li Linlin Fan Feifei Shang Yuqiao Zhang Liang Shuai Yuhua xie Zhenhua Duan |
| author_sort | Lili Li |
| collection | DOAJ |
| description | Electron beam irradiation is a physical fungicidal technique that has emerged as a potential application in China. However, its antifungal activity and mechanism against Rhizopus oryzae have not been reported. Thus, this study aimed to investigate the antifungal activity and mechanism of electron beam irradiation of R. oryzae. The antifungal activity analysis showed that the D10 value and complete elimination dose of R. oryzae irradiated by electron beam were 1.73 kGy and 8.08 kGy, respectively. Electron beam irradiation has a strong inhibitory effect on the filamentous biomass of R. oryzae. To reveal the antifungal mechanism of electron beam against R. oryzae, this study analyzed the dynamic changes in the cell wall, cell membrane, and oxidative stress induced by different irradiation doses. The results showed that electron beam irradiation destroyed the cell wall structure of R. oryzae, increasing chitinase activity and decreasing chitin content. Cell membrane integrity is disrupted, increasing relative conductivity, decreasing pH values, and decreasing soluble protein content. Electron beam irradiation causes oxidative stress in cells, increasing H2O2 content, decreasing antisuperoxide anion activity, decreasing DPPH free radical scavenging activity, and inhibiting defense enzyme (CAT and SOD) activity. This phenomenon indicates that electron beams can cause structural damage to and metabolic dysfunction of cells and disorders of redox homeostasis, which may be the main cause of growth inhibition and cell death in R. oryzae. |
| first_indexed | 2024-03-13T01:36:30Z |
| format | Article |
| id | doaj.art-12962a1975d648f1ba4b59d6f219cfae |
| institution | Directory Open Access Journal |
| issn | 0362-028X |
| language | English |
| last_indexed | 2024-03-13T01:36:30Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj.art-12962a1975d648f1ba4b59d6f219cfae2023-07-04T05:08:08ZengElsevierJournal of Food Protection0362-028X2023-05-01865100070Antifungal Activity and Mechanism of Electron Beam Irradiation Against Rhizopus oryzaeLili Li0Linlin Fan1Feifei Shang2Yuqiao Zhang3Liang Shuai4Yuhua xie5Zhenhua Duan6Food and Biological Engineering/Guangxi Key Laboratory of Health Care Food science and Technology, Hezhou University, Hezhou 542899, China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaFood and Biological Engineering/Guangxi Key Laboratory of Health Care Food science and Technology, Hezhou University, Hezhou 542899, China; College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzghou 545006, ChinaFood and Biological Engineering/Guangxi Key Laboratory of Health Care Food science and Technology, Hezhou University, Hezhou 542899, China; Corresponding author.Food and Biological Engineering/Guangxi Key Laboratory of Health Care Food science and Technology, Hezhou University, Hezhou 542899, China; College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzghou 545006, ChinaFood and Biological Engineering/Guangxi Key Laboratory of Health Care Food science and Technology, Hezhou University, Hezhou 542899, ChinaFood and Biological Engineering/Guangxi Key Laboratory of Health Care Food science and Technology, Hezhou University, Hezhou 542899, ChinaFood and Biological Engineering/Guangxi Key Laboratory of Health Care Food science and Technology, Hezhou University, Hezhou 542899, China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaElectron beam irradiation is a physical fungicidal technique that has emerged as a potential application in China. However, its antifungal activity and mechanism against Rhizopus oryzae have not been reported. Thus, this study aimed to investigate the antifungal activity and mechanism of electron beam irradiation of R. oryzae. The antifungal activity analysis showed that the D10 value and complete elimination dose of R. oryzae irradiated by electron beam were 1.73 kGy and 8.08 kGy, respectively. Electron beam irradiation has a strong inhibitory effect on the filamentous biomass of R. oryzae. To reveal the antifungal mechanism of electron beam against R. oryzae, this study analyzed the dynamic changes in the cell wall, cell membrane, and oxidative stress induced by different irradiation doses. The results showed that electron beam irradiation destroyed the cell wall structure of R. oryzae, increasing chitinase activity and decreasing chitin content. Cell membrane integrity is disrupted, increasing relative conductivity, decreasing pH values, and decreasing soluble protein content. Electron beam irradiation causes oxidative stress in cells, increasing H2O2 content, decreasing antisuperoxide anion activity, decreasing DPPH free radical scavenging activity, and inhibiting defense enzyme (CAT and SOD) activity. This phenomenon indicates that electron beams can cause structural damage to and metabolic dysfunction of cells and disorders of redox homeostasis, which may be the main cause of growth inhibition and cell death in R. oryzae.http://www.sciencedirect.com/science/article/pii/S0362028X2306742XAntifungal activityAntifungal mechanismElectron beam irradiationOxidative metabolismRhizopus oryzae |
| spellingShingle | Lili Li Linlin Fan Feifei Shang Yuqiao Zhang Liang Shuai Yuhua xie Zhenhua Duan Antifungal Activity and Mechanism of Electron Beam Irradiation Against Rhizopus oryzae Journal of Food Protection Antifungal activity Antifungal mechanism Electron beam irradiation Oxidative metabolism Rhizopus oryzae |
| title | Antifungal Activity and Mechanism of Electron Beam Irradiation Against Rhizopus oryzae |
| title_full | Antifungal Activity and Mechanism of Electron Beam Irradiation Against Rhizopus oryzae |
| title_fullStr | Antifungal Activity and Mechanism of Electron Beam Irradiation Against Rhizopus oryzae |
| title_full_unstemmed | Antifungal Activity and Mechanism of Electron Beam Irradiation Against Rhizopus oryzae |
| title_short | Antifungal Activity and Mechanism of Electron Beam Irradiation Against Rhizopus oryzae |
| title_sort | antifungal activity and mechanism of electron beam irradiation against rhizopus oryzae |
| topic | Antifungal activity Antifungal mechanism Electron beam irradiation Oxidative metabolism Rhizopus oryzae |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X2306742X |
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