Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice
Administration of freeze-dried powder of Saskatoon berry (SB), a popular fruit enriched with antioxidants, reduced glucose level, inflammatory markers and gut microbiota disorder in high fat-high sucrose (HFHS) diet-induced insulin resistant mice. The present study examined the dose-response relatio...
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MDPI AG
2021-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/8/1553 |
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| author | Ruozhi Zhao Fei Huang Garry X. Shen |
| author_facet | Ruozhi Zhao Fei Huang Garry X. Shen |
| author_sort | Ruozhi Zhao |
| collection | DOAJ |
| description | Administration of freeze-dried powder of Saskatoon berry (SB), a popular fruit enriched with antioxidants, reduced glucose level, inflammatory markers and gut microbiota disorder in high fat-high sucrose (HFHS) diet-induced insulin resistant mice. The present study examined the dose-response relationship in metabolic, inflammatory and gut microbiotic variables to SB power (SBp) supplementation in HFHS diet-fed mice. Male C57 BL/6J mice were fed with HFHS diet supplemented with 0, 1%, 2.5% or 5% SBp for 11 weeks. HFHS diet significantly increased the levels of fast plasma glucose (FPG), cholesterol, triglycerides, insulin, homeostatic model assessment of insulin resistance (HOMA-IR), tumor necrosis factor-α, monocyte chemotactic protein-1 and plasminogen activator inhibitor-1, but decreased fecal <i>Bacteroidetes</i> phylum bacteria and <i>Muribaculaceae</i> family bacteria compared to low fat diet. SBp dose-dependently reduced metabolic and inflammatory variables and gut dysbiosis in mice compared with mice receiving HFHS diet alone. Significant attenuation of HFHS diet-induced biochemical disorders were detected in mice receiving ≥1% SBp. The abundances of <i>Muribaculaceae</i> family bacteria negatively correlated with body weights, FPG, lipids, insulin, HOMA-IR and inflammatory markers in the mice. The results suggest that SBp supplementation dose-dependently attenuated HFHS diet-induced metabolic and inflammatory disorders, which was associated with the amelioration of gut dysbiosis in the mice. |
| first_indexed | 2024-03-10T08:34:49Z |
| format | Article |
| id | doaj.art-12978adcdead4c149481c3befc0f8c81 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:34:49Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-12978adcdead4c149481c3befc0f8c812023-11-22T08:45:26ZengMDPI AGMicroorganisms2076-26072021-07-0198155310.3390/microorganisms9081553Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant MiceRuozhi Zhao0Fei Huang1Garry X. Shen2Department of Internal Medicine, University of Manitoba, Winnipeg, MB R3E 3P4, CanadaDepartment of Internal Medicine, University of Manitoba, Winnipeg, MB R3E 3P4, CanadaDepartment of Internal Medicine, University of Manitoba, Winnipeg, MB R3E 3P4, CanadaAdministration of freeze-dried powder of Saskatoon berry (SB), a popular fruit enriched with antioxidants, reduced glucose level, inflammatory markers and gut microbiota disorder in high fat-high sucrose (HFHS) diet-induced insulin resistant mice. The present study examined the dose-response relationship in metabolic, inflammatory and gut microbiotic variables to SB power (SBp) supplementation in HFHS diet-fed mice. Male C57 BL/6J mice were fed with HFHS diet supplemented with 0, 1%, 2.5% or 5% SBp for 11 weeks. HFHS diet significantly increased the levels of fast plasma glucose (FPG), cholesterol, triglycerides, insulin, homeostatic model assessment of insulin resistance (HOMA-IR), tumor necrosis factor-α, monocyte chemotactic protein-1 and plasminogen activator inhibitor-1, but decreased fecal <i>Bacteroidetes</i> phylum bacteria and <i>Muribaculaceae</i> family bacteria compared to low fat diet. SBp dose-dependently reduced metabolic and inflammatory variables and gut dysbiosis in mice compared with mice receiving HFHS diet alone. Significant attenuation of HFHS diet-induced biochemical disorders were detected in mice receiving ≥1% SBp. The abundances of <i>Muribaculaceae</i> family bacteria negatively correlated with body weights, FPG, lipids, insulin, HOMA-IR and inflammatory markers in the mice. The results suggest that SBp supplementation dose-dependently attenuated HFHS diet-induced metabolic and inflammatory disorders, which was associated with the amelioration of gut dysbiosis in the mice.https://www.mdpi.com/2076-2607/9/8/1553Saskatoon berrydose-responsemicehigh fat-high sucrose dietfasting plasma glucoseinflammation |
| spellingShingle | Ruozhi Zhao Fei Huang Garry X. Shen Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice Microorganisms Saskatoon berry dose-response mice high fat-high sucrose diet fasting plasma glucose inflammation |
| title | Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice |
| title_full | Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice |
| title_fullStr | Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice |
| title_full_unstemmed | Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice |
| title_short | Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice |
| title_sort | dose responses relationship in glucose lowering and gut dysbiosis to saskatoon berry powder supplementation in high fat high sucrose diet induced insulin resistant mice |
| topic | Saskatoon berry dose-response mice high fat-high sucrose diet fasting plasma glucose inflammation |
| url | https://www.mdpi.com/2076-2607/9/8/1553 |
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