Enhanced Effect of Polyethyleneimine-Modified Graphene Oxide and Simvastatin on Osteogenic Differentiation of Murine Bone Marrow-Derived Mesenchymal Stem Cells
Statin derivatives traditionally have been used for the treatment of hyperlipidemia, but recent studies have shown their ability to regulate bone metabolism and promote bone growth. In this study, simvastatin (Sim), a new therapeutic candidate for bone regeneration, was combined with graphene oxide...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/5/501 |
| _version_ | 1827693466241466368 |
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| author | Jun-Sung Oh Jeong-Sun Park Eun-Jung Lee |
| author_facet | Jun-Sung Oh Jeong-Sun Park Eun-Jung Lee |
| author_sort | Jun-Sung Oh |
| collection | DOAJ |
| description | Statin derivatives traditionally have been used for the treatment of hyperlipidemia, but recent studies have shown their ability to regulate bone metabolism and promote bone growth. In this study, simvastatin (Sim), a new therapeutic candidate for bone regeneration, was combined with graphene oxide (GO), which has recently attracted much interest as a drug delivery method, to produce a compound substance effective for bone regeneration. To create a stable and homogenous complex with Sim, GO was modified with polyethylenimine, and the effect of modification was analyzed using Fourier transform infrared spectroscopy, zeta potential, and cytotoxicity testing. More specifically, the osteogenic differentiation potential expected by the combination of the two effective materials for osteogenic differentiation, GO and Sim, was evaluated in mesenchymal stem cells. Compared with control groups with GO and Sim used separately, the GO/Sim complex showed excellent osteogenic differentiation properties, with especially enhanced effects in the complex containing < 1 μM Sim. |
| first_indexed | 2024-03-10T11:43:59Z |
| format | Article |
| id | doaj.art-1297e9aaaae34315bcf6c6ddd4ca8c71 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T11:43:59Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-1297e9aaaae34315bcf6c6ddd4ca8c712023-11-21T18:12:19ZengMDPI AGBiomedicines2227-90592021-05-019550110.3390/biomedicines9050501Enhanced Effect of Polyethyleneimine-Modified Graphene Oxide and Simvastatin on Osteogenic Differentiation of Murine Bone Marrow-Derived Mesenchymal Stem CellsJun-Sung Oh0Jeong-Sun Park1Eun-Jung Lee2Department of Nano-biomedical Science, Dankook University, Cheonan 31116, KoreaDepartment of Nano-biomedical Science, Dankook University, Cheonan 31116, KoreaDepartment of Nano-biomedical Science, Dankook University, Cheonan 31116, KoreaStatin derivatives traditionally have been used for the treatment of hyperlipidemia, but recent studies have shown their ability to regulate bone metabolism and promote bone growth. In this study, simvastatin (Sim), a new therapeutic candidate for bone regeneration, was combined with graphene oxide (GO), which has recently attracted much interest as a drug delivery method, to produce a compound substance effective for bone regeneration. To create a stable and homogenous complex with Sim, GO was modified with polyethylenimine, and the effect of modification was analyzed using Fourier transform infrared spectroscopy, zeta potential, and cytotoxicity testing. More specifically, the osteogenic differentiation potential expected by the combination of the two effective materials for osteogenic differentiation, GO and Sim, was evaluated in mesenchymal stem cells. Compared with control groups with GO and Sim used separately, the GO/Sim complex showed excellent osteogenic differentiation properties, with especially enhanced effects in the complex containing < 1 μM Sim.https://www.mdpi.com/2227-9059/9/5/501simvastatingraphene oxidepolyethyleniminemesenchymal stem cellsosteogenic differentiation |
| spellingShingle | Jun-Sung Oh Jeong-Sun Park Eun-Jung Lee Enhanced Effect of Polyethyleneimine-Modified Graphene Oxide and Simvastatin on Osteogenic Differentiation of Murine Bone Marrow-Derived Mesenchymal Stem Cells Biomedicines simvastatin graphene oxide polyethylenimine mesenchymal stem cells osteogenic differentiation |
| title | Enhanced Effect of Polyethyleneimine-Modified Graphene Oxide and Simvastatin on Osteogenic Differentiation of Murine Bone Marrow-Derived Mesenchymal Stem Cells |
| title_full | Enhanced Effect of Polyethyleneimine-Modified Graphene Oxide and Simvastatin on Osteogenic Differentiation of Murine Bone Marrow-Derived Mesenchymal Stem Cells |
| title_fullStr | Enhanced Effect of Polyethyleneimine-Modified Graphene Oxide and Simvastatin on Osteogenic Differentiation of Murine Bone Marrow-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Enhanced Effect of Polyethyleneimine-Modified Graphene Oxide and Simvastatin on Osteogenic Differentiation of Murine Bone Marrow-Derived Mesenchymal Stem Cells |
| title_short | Enhanced Effect of Polyethyleneimine-Modified Graphene Oxide and Simvastatin on Osteogenic Differentiation of Murine Bone Marrow-Derived Mesenchymal Stem Cells |
| title_sort | enhanced effect of polyethyleneimine modified graphene oxide and simvastatin on osteogenic differentiation of murine bone marrow derived mesenchymal stem cells |
| topic | simvastatin graphene oxide polyethylenimine mesenchymal stem cells osteogenic differentiation |
| url | https://www.mdpi.com/2227-9059/9/5/501 |
| work_keys_str_mv | AT junsungoh enhancedeffectofpolyethyleneiminemodifiedgrapheneoxideandsimvastatinonosteogenicdifferentiationofmurinebonemarrowderivedmesenchymalstemcells AT jeongsunpark enhancedeffectofpolyethyleneiminemodifiedgrapheneoxideandsimvastatinonosteogenicdifferentiationofmurinebonemarrowderivedmesenchymalstemcells AT eunjunglee enhancedeffectofpolyethyleneiminemodifiedgrapheneoxideandsimvastatinonosteogenicdifferentiationofmurinebonemarrowderivedmesenchymalstemcells |