Cloning and expression of Bacillus subtilis genes in Streptomyces sp.
A local isolate Bacillus subtilis was used, which producing thennophilic complex enzyme having similar activity of endogluganase enzyme ( Endo-l,4-B-Dglucanase ). Partially digested chromosomal DNA of Bacillus subtilis by Eco Rl restriction enzyme randomly cloned i...
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| Format: | Article |
| Language: | English |
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University of Baghdad
2017-09-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/1346 |
| _version_ | 1818270239639470080 |
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| author | E. D. Salman |
| author_facet | E. D. Salman |
| author_sort | E. D. Salman |
| collection | DOAJ |
| description |
A local isolate Bacillus subtilis was used, which producing
thennophilic complex enzyme having similar activity of endogluganase
enzyme ( Endo-l,4-B-Dglucanase ).
Partially digested chromosomal DNA of Bacillus subtilis by Eco
Rl restriction enzyme randomly cloned into Eco Rl pSU10l shuttle vector. The resulted hybrid plasmid was transformed into protoplast of
Streptomyces sp. SH-H.
The result revealed that cellulose genes, which expressed into transfonned cells are located on chromosomal DNA fragment having size
4.5·5 kb in size.
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| first_indexed | 2024-12-12T21:07:07Z |
| format | Article |
| id | doaj.art-12984fce86c04696a8e4c62ca04075bd |
| institution | Directory Open Access Journal |
| issn | 1609-4042 2521-3407 |
| language | English |
| last_indexed | 2024-12-12T21:07:07Z |
| publishDate | 2017-09-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj.art-12984fce86c04696a8e4c62ca04075bd2022-12-22T00:11:59ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072017-09-01212Cloning and expression of Bacillus subtilis genes in Streptomyces sp.E. D. Salman A local isolate Bacillus subtilis was used, which producing thennophilic complex enzyme having similar activity of endogluganase enzyme ( Endo-l,4-B-Dglucanase ). Partially digested chromosomal DNA of Bacillus subtilis by Eco Rl restriction enzyme randomly cloned into Eco Rl pSU10l shuttle vector. The resulted hybrid plasmid was transformed into protoplast of Streptomyces sp. SH-H. The result revealed that cellulose genes, which expressed into transfonned cells are located on chromosomal DNA fragment having size 4.5·5 kb in size. https://jih.uobaghdad.edu.iq/index.php/j/article/view/1346 |
| spellingShingle | E. D. Salman Cloning and expression of Bacillus subtilis genes in Streptomyces sp. Ibn Al-Haitham Journal for Pure and Applied Sciences |
| title | Cloning and expression of Bacillus subtilis genes in Streptomyces sp. |
| title_full | Cloning and expression of Bacillus subtilis genes in Streptomyces sp. |
| title_fullStr | Cloning and expression of Bacillus subtilis genes in Streptomyces sp. |
| title_full_unstemmed | Cloning and expression of Bacillus subtilis genes in Streptomyces sp. |
| title_short | Cloning and expression of Bacillus subtilis genes in Streptomyces sp. |
| title_sort | cloning and expression of bacillus subtilis genes in streptomyces sp |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/1346 |
| work_keys_str_mv | AT edsalman cloningandexpressionofbacillussubtilisgenesinstreptomycessp |