Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs
Proper skin barrier function is paramount for our survival, and, suffering injury, there is an acute need to restore the lost barrier and prevent development of a chronic wound. We hypothesize that rapid wound closure is more important than immediate perfection of the barrier, whereas specific treat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/4/360 |
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| author | Enamul Haque Mojumdar Lone Bruhn Madsen Henri Hansson Ida Taavoniku Klaus Kristensen Christina Persson Anna Karin Morén Rajmund Mokso Artur Schmidtchen Tautgirdas Ruzgas Johan Engblom |
| author_facet | Enamul Haque Mojumdar Lone Bruhn Madsen Henri Hansson Ida Taavoniku Klaus Kristensen Christina Persson Anna Karin Morén Rajmund Mokso Artur Schmidtchen Tautgirdas Ruzgas Johan Engblom |
| author_sort | Enamul Haque Mojumdar |
| collection | DOAJ |
| description | Proper skin barrier function is paramount for our survival, and, suffering injury, there is an acute need to restore the lost barrier and prevent development of a chronic wound. We hypothesize that rapid wound closure is more important than immediate perfection of the barrier, whereas specific treatment may facilitate perfection. The aim of the current project was therefore to evaluate the quality of restored tissue down to the molecular level. We used Göttingen minipigs with a multi-technique approach correlating wound healing progression in vivo over three weeks, monitored by classical methods (e.g., histology, trans-epidermal water loss (TEWL), pH) and subsequent physicochemical characterization of barrier recovery (i.e., small and wide-angle X-ray diffraction (SWAXD), polarization transfer solid-state NMR (PTssNMR), dynamic vapor sorption (DVS), Fourier transform infrared (FTIR)), providing a unique insight into molecular aspects of healing. We conclude that although acute wounds sealed within two weeks as expected, molecular investigation of stratum corneum (SC) revealed a poorly developed keratin organization and deviations in lipid lamellae formation. A higher lipid fluidity was also observed in regenerated tissue. This may have been due to incomplete lipid conversion during barrier recovery as glycosphingolipids, normally not present in SC, were indicated by infrared FTIR spectroscopy. Evidently, a molecular approach to skin barrier recovery could be a valuable tool in future development of products targeting wound healing. |
| first_indexed | 2024-03-10T12:45:10Z |
| format | Article |
| id | doaj.art-12985f8705c6402d96e07aa7661181dd |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T12:45:10Z |
| publishDate | 2021-03-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-12985f8705c6402d96e07aa7661181dd2023-11-21T13:33:36ZengMDPI AGBiomedicines2227-90592021-03-019436010.3390/biomedicines9040360Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on PigsEnamul Haque Mojumdar0Lone Bruhn Madsen1Henri Hansson2Ida Taavoniku3Klaus Kristensen4Christina Persson5Anna Karin Morén6Rajmund Mokso7Artur Schmidtchen8Tautgirdas Ruzgas9Johan Engblom10Department of Biomedical Science, Faculty of Health and Society, Malmö University, SE-205 06 Malmö, SwedenTimeline Bioresearch AB, Scheelevägen 2, SE-223 63 Lund, SwedenGalenica AB, Medeon Science Park, SE-205 12 Malmö, SwedenTimeline Bioresearch AB, Scheelevägen 2, SE-223 63 Lund, SwedenTimeline Bioresearch AB, Scheelevägen 2, SE-223 63 Lund, SwedenDepartment of Occupational and Environmental Dermatology, Lund University, Skåne University Hospital, SE-205 02 Malmö, SwedenGalenica AB, Medeon Science Park, SE-205 12 Malmö, SwedenDepartment of Solid Mechanics & MAX IV Laboratory, Lund University, SE-221 00 Lund, SwedenDivision of Dermatology and Venereology, Department of Clinical Sciences, Lund University, SE-221 84 Lund, SwedenDepartment of Biomedical Science, Faculty of Health and Society, Malmö University, SE-205 06 Malmö, SwedenDepartment of Biomedical Science, Faculty of Health and Society, Malmö University, SE-205 06 Malmö, SwedenProper skin barrier function is paramount for our survival, and, suffering injury, there is an acute need to restore the lost barrier and prevent development of a chronic wound. We hypothesize that rapid wound closure is more important than immediate perfection of the barrier, whereas specific treatment may facilitate perfection. The aim of the current project was therefore to evaluate the quality of restored tissue down to the molecular level. We used Göttingen minipigs with a multi-technique approach correlating wound healing progression in vivo over three weeks, monitored by classical methods (e.g., histology, trans-epidermal water loss (TEWL), pH) and subsequent physicochemical characterization of barrier recovery (i.e., small and wide-angle X-ray diffraction (SWAXD), polarization transfer solid-state NMR (PTssNMR), dynamic vapor sorption (DVS), Fourier transform infrared (FTIR)), providing a unique insight into molecular aspects of healing. We conclude that although acute wounds sealed within two weeks as expected, molecular investigation of stratum corneum (SC) revealed a poorly developed keratin organization and deviations in lipid lamellae formation. A higher lipid fluidity was also observed in regenerated tissue. This may have been due to incomplete lipid conversion during barrier recovery as glycosphingolipids, normally not present in SC, were indicated by infrared FTIR spectroscopy. Evidently, a molecular approach to skin barrier recovery could be a valuable tool in future development of products targeting wound healing.https://www.mdpi.com/2227-9059/9/4/360skin barrierstratum corneumlipidacute woundin vivo/ex vivotrans-epidermal water loss (TEWL) |
| spellingShingle | Enamul Haque Mojumdar Lone Bruhn Madsen Henri Hansson Ida Taavoniku Klaus Kristensen Christina Persson Anna Karin Morén Rajmund Mokso Artur Schmidtchen Tautgirdas Ruzgas Johan Engblom Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs Biomedicines skin barrier stratum corneum lipid acute wound in vivo/ex vivo trans-epidermal water loss (TEWL) |
| title | Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs |
| title_full | Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs |
| title_fullStr | Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs |
| title_full_unstemmed | Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs |
| title_short | Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs |
| title_sort | probing skin barrier recovery on molecular level following acute wounds an in vivo ex vivo study on pigs |
| topic | skin barrier stratum corneum lipid acute wound in vivo/ex vivo trans-epidermal water loss (TEWL) |
| url | https://www.mdpi.com/2227-9059/9/4/360 |
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