Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection
Prostate cancer can be detected early by testing the presence of prostate-specific antigen (PSA) in the blood. Lateral flow immunoassay (LFIA) has been used because it is cost effective and easy to use and also has a rapid sample-to-answer process. Quantum dots (QDs) with very bright fluorescence ha...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/1/33 |
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| author | Sungje Bock Hyung-Mo Kim Jaehi Kim Jaehyun An Yun-Sik Choi Xuan-Hung Pham Ahla Jo Kyeong-min Ham Hobeom Song Jung-Won Kim Eunil Hahm Won-Yeop Rho Sang Hun Lee Seung-min Park Sangchul Lee Dae Hong Jeong Ho-Young Lee Bong-Hyun Jun |
| author_facet | Sungje Bock Hyung-Mo Kim Jaehi Kim Jaehyun An Yun-Sik Choi Xuan-Hung Pham Ahla Jo Kyeong-min Ham Hobeom Song Jung-Won Kim Eunil Hahm Won-Yeop Rho Sang Hun Lee Seung-min Park Sangchul Lee Dae Hong Jeong Ho-Young Lee Bong-Hyun Jun |
| author_sort | Sungje Bock |
| collection | DOAJ |
| description | Prostate cancer can be detected early by testing the presence of prostate-specific antigen (PSA) in the blood. Lateral flow immunoassay (LFIA) has been used because it is cost effective and easy to use and also has a rapid sample-to-answer process. Quantum dots (QDs) with very bright fluorescence have been previously used to improve the detection sensitivity of LFIAs. In the current study, a highly sensitive LFIA kit was devised using QD-embedded silica nanoparticles. In the present study, only a smartphone and a computer software program, ImageJ, were used, because the developed system had high sensitivity by using very bright nanoprobes. The limit of PSA detection of the developed LFIA system was 0.138 ng/mL. The area under the curve of this system was calculated as 0.852. The system did not show any false-negative result when 47 human serum samples were analyzed; it only detected PSA and did not detect alpha-fetoprotein and newborn calf serum in the samples. Additionally, fluorescence was maintained on the strip for 10 d after the test. With its high sensitivity and convenience, the devised LFIA kit can be used for the diagnosis of prostate cancer. |
| first_indexed | 2024-03-10T03:28:39Z |
| format | Article |
| id | doaj.art-1298ff80db524adb972b60ac4ba0f5f9 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:28:39Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-1298ff80db524adb972b60ac4ba0f5f92023-11-23T12:00:33ZengMDPI AGNanomaterials2079-49912021-12-011213310.3390/nano12010033Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen DetectionSungje Bock0Hyung-Mo Kim1Jaehi Kim2Jaehyun An3Yun-Sik Choi4Xuan-Hung Pham5Ahla Jo6Kyeong-min Ham7Hobeom Song8Jung-Won Kim9Eunil Hahm10Won-Yeop Rho11Sang Hun Lee12Seung-min Park13Sangchul Lee14Dae Hong Jeong15Ho-Young Lee16Bong-Hyun Jun17Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Chemistry Education, Seoul National University, Seoul 08826, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaBioSquare Inc., Hwaseong 18449, KoreaBioSquare Inc., Hwaseong 18449, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaSchool of International Engineering and Science, Jeonbuk National University, Jeonju 54896, KoreaDepartment of Chemical and Biological Engineering, Hanbat National University, Deajeon 34158, KoreaDepartment of Urology, Stanford University School of Medicine, Palo Alto, CA 94305, USADepartment of Urology, Seoul National University Bundang Hospital, Seongnam 13620, KoreaDepartment of Chemistry Education, Seoul National University, Seoul 08826, KoreaDepartment of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam 13620, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaProstate cancer can be detected early by testing the presence of prostate-specific antigen (PSA) in the blood. Lateral flow immunoassay (LFIA) has been used because it is cost effective and easy to use and also has a rapid sample-to-answer process. Quantum dots (QDs) with very bright fluorescence have been previously used to improve the detection sensitivity of LFIAs. In the current study, a highly sensitive LFIA kit was devised using QD-embedded silica nanoparticles. In the present study, only a smartphone and a computer software program, ImageJ, were used, because the developed system had high sensitivity by using very bright nanoprobes. The limit of PSA detection of the developed LFIA system was 0.138 ng/mL. The area under the curve of this system was calculated as 0.852. The system did not show any false-negative result when 47 human serum samples were analyzed; it only detected PSA and did not detect alpha-fetoprotein and newborn calf serum in the samples. Additionally, fluorescence was maintained on the strip for 10 d after the test. With its high sensitivity and convenience, the devised LFIA kit can be used for the diagnosis of prostate cancer.https://www.mdpi.com/2079-4991/12/1/33prostate-specific antigenprostate cancerlateral flow immunoassayquantum dotquantum-dot-embedded silica nanoparticles |
| spellingShingle | Sungje Bock Hyung-Mo Kim Jaehi Kim Jaehyun An Yun-Sik Choi Xuan-Hung Pham Ahla Jo Kyeong-min Ham Hobeom Song Jung-Won Kim Eunil Hahm Won-Yeop Rho Sang Hun Lee Seung-min Park Sangchul Lee Dae Hong Jeong Ho-Young Lee Bong-Hyun Jun Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection Nanomaterials prostate-specific antigen prostate cancer lateral flow immunoassay quantum dot quantum-dot-embedded silica nanoparticles |
| title | Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection |
| title_full | Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection |
| title_fullStr | Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection |
| title_full_unstemmed | Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection |
| title_short | Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection |
| title_sort | lateral flow immunoassay with quantum dot embedded silica nanoparticles for prostate specific antigen detection |
| topic | prostate-specific antigen prostate cancer lateral flow immunoassay quantum dot quantum-dot-embedded silica nanoparticles |
| url | https://www.mdpi.com/2079-4991/12/1/33 |
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