The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based study
Objective: To explore the effect and mechanisms of a traditional Chinese quadri-combination therapy [Bushen, Yiqi, Lixue and Yangtai (BYLY)] in treating recurrent spontaneous abortion (RSA).Methods: A clinical trial was conducted to study the effect of BYLY on RSA. Pharmacological network analysis a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.965694/full |
| _version_ | 1828323552438255616 |
|---|---|
| author | Jing Zhou Jing Zhou Jing Zhou Lisha Li Lisha Li Lisha Li Xinyao Pan Xinyao Pan Xinyao Pan Jing Wang Jing Wang Jing Wang Qing Qi Qing Qi Qing Qi Hongmei Sun Hongmei Sun Hongmei Sun Chuyu Li Chuyu Li Chuyu Li Ling Wang Ling Wang Ling Wang |
| author_facet | Jing Zhou Jing Zhou Jing Zhou Lisha Li Lisha Li Lisha Li Xinyao Pan Xinyao Pan Xinyao Pan Jing Wang Jing Wang Jing Wang Qing Qi Qing Qi Qing Qi Hongmei Sun Hongmei Sun Hongmei Sun Chuyu Li Chuyu Li Chuyu Li Ling Wang Ling Wang Ling Wang |
| author_sort | Jing Zhou |
| collection | DOAJ |
| description | Objective: To explore the effect and mechanisms of a traditional Chinese quadri-combination therapy [Bushen, Yiqi, Lixue and Yangtai (BYLY)] in treating recurrent spontaneous abortion (RSA).Methods: A clinical trial was conducted to study the effect of BYLY on RSA. Pharmacological network analysis and UPLC-Q/TOF-mass spectrometry (MS) were applied to investigate the key active component in BYLY and potential targets. Cellular experiments based on former results were performed to examine the mechanism of BYLY in the treatment of RSA.Results: Four hundred and eighty participants enrolled in the clinical trial. The results showed that, compared with the use of BYLY or duphaston alone, a combination of duphaston and BYLY could decrease the early abortion rate in RSA (p < 0.001). Network pharmacological analysis indicated that BYLY contained 132 active components and 146 core targets, and the quercetin maybe the key effective component. In vitro experiments found that pretreatment of quercetin at the correct concentration (2 μM) prevented hypoxia-induced viability and proliferation reduction, and apoptosis and mitochondrial dysfunction. Furthermore, quercetin could modulate mitochondrial fission/fusion balance in trophoblasts, and specifically decrease the expression of Drp1 by regulating miR-34a-5p.Conclusion: BYLY could improve pregnancy outcomes of RSA, based on multi-components and multi-targets. The protective effect of quercetin on trophoblasts, through decreasing Drp1 expression via regulating miR-34a-5p, might be one possible effective mechanism. |
| first_indexed | 2024-04-13T18:54:14Z |
| format | Article |
| id | doaj.art-12997a43b2924733b14065d9bd96d294 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T18:54:14Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-12997a43b2924733b14065d9bd96d2942022-12-22T02:34:18ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-10-011310.3389/fphar.2022.965694965694The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based studyJing Zhou0Jing Zhou1Jing Zhou2Lisha Li3Lisha Li4Lisha Li5Xinyao Pan6Xinyao Pan7Xinyao Pan8Jing Wang9Jing Wang10Jing Wang11Qing Qi12Qing Qi13Qing Qi14Hongmei Sun15Hongmei Sun16Hongmei Sun17Chuyu Li18Chuyu Li19Chuyu Li20Ling Wang21Ling Wang22Ling Wang23Laboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, ChinaThe Academy of Integrative Medicine of Fudan University, Shanghai, ChinaShanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, ChinaLaboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, ChinaThe Academy of Integrative Medicine of Fudan University, Shanghai, ChinaShanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, ChinaLaboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, ChinaThe Academy of Integrative Medicine of Fudan University, Shanghai, ChinaShanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, ChinaLaboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, ChinaThe Academy of Integrative Medicine of Fudan University, Shanghai, ChinaShanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, ChinaLaboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, ChinaThe Academy of Integrative Medicine of Fudan University, Shanghai, ChinaShanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, ChinaLaboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, ChinaThe Academy of Integrative Medicine of Fudan University, Shanghai, ChinaShanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, ChinaLaboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, ChinaThe Academy of Integrative Medicine of Fudan University, Shanghai, ChinaShanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, ChinaLaboratory for Reproductive Immunology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, ChinaThe Academy of Integrative Medicine of Fudan University, Shanghai, ChinaShanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, ChinaObjective: To explore the effect and mechanisms of a traditional Chinese quadri-combination therapy [Bushen, Yiqi, Lixue and Yangtai (BYLY)] in treating recurrent spontaneous abortion (RSA).Methods: A clinical trial was conducted to study the effect of BYLY on RSA. Pharmacological network analysis and UPLC-Q/TOF-mass spectrometry (MS) were applied to investigate the key active component in BYLY and potential targets. Cellular experiments based on former results were performed to examine the mechanism of BYLY in the treatment of RSA.Results: Four hundred and eighty participants enrolled in the clinical trial. The results showed that, compared with the use of BYLY or duphaston alone, a combination of duphaston and BYLY could decrease the early abortion rate in RSA (p < 0.001). Network pharmacological analysis indicated that BYLY contained 132 active components and 146 core targets, and the quercetin maybe the key effective component. In vitro experiments found that pretreatment of quercetin at the correct concentration (2 μM) prevented hypoxia-induced viability and proliferation reduction, and apoptosis and mitochondrial dysfunction. Furthermore, quercetin could modulate mitochondrial fission/fusion balance in trophoblasts, and specifically decrease the expression of Drp1 by regulating miR-34a-5p.Conclusion: BYLY could improve pregnancy outcomes of RSA, based on multi-components and multi-targets. The protective effect of quercetin on trophoblasts, through decreasing Drp1 expression via regulating miR-34a-5p, might be one possible effective mechanism.https://www.frontiersin.org/articles/10.3389/fphar.2022.965694/fullquadri-combination therapyrecurrent spontaneous abortionquercetinmitochondrial dynamicsDRP1miR-34a-5p |
| spellingShingle | Jing Zhou Jing Zhou Jing Zhou Lisha Li Lisha Li Lisha Li Xinyao Pan Xinyao Pan Xinyao Pan Jing Wang Jing Wang Jing Wang Qing Qi Qing Qi Qing Qi Hongmei Sun Hongmei Sun Hongmei Sun Chuyu Li Chuyu Li Chuyu Li Ling Wang Ling Wang Ling Wang The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based study Frontiers in Pharmacology quadri-combination therapy recurrent spontaneous abortion quercetin mitochondrial dynamics DRP1 miR-34a-5p |
| title | The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based study |
| title_full | The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based study |
| title_fullStr | The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based study |
| title_full_unstemmed | The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based study |
| title_short | The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based study |
| title_sort | effect of a traditional chinese quadri combination therapy and its component quercetin on recurrent spontaneous abortion a clinical trial network pharmacology and experiments based study |
| topic | quadri-combination therapy recurrent spontaneous abortion quercetin mitochondrial dynamics DRP1 miR-34a-5p |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.965694/full |
| work_keys_str_mv | AT jingzhou theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingzhou theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingzhou theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lishali theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lishali theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lishali theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT xinyaopan theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT xinyaopan theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT xinyaopan theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingwang theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingwang theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingwang theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT qingqi theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT qingqi theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT qingqi theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT hongmeisun theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT hongmeisun theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT hongmeisun theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT chuyuli theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT chuyuli theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT chuyuli theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lingwang theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lingwang theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lingwang theeffectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingzhou effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingzhou effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingzhou effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lishali effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lishali effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lishali effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT xinyaopan effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT xinyaopan effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT xinyaopan effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingwang effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingwang effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT jingwang effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT qingqi effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT qingqi effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT qingqi effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT hongmeisun effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT hongmeisun effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT hongmeisun effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT chuyuli effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT chuyuli effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT chuyuli effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lingwang effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lingwang effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy AT lingwang effectofatraditionalchinesequadricombinationtherapyanditscomponentquercetinonrecurrentspontaneousabortionaclinicaltrialnetworkpharmacologyandexperimentsbasedstudy |