IDENTIFICATION OF NEW POLISH LINES OF CHENOPODIUM QUINOA (WILLD.) BY SPECTRAL ANALYSIS OF PIGMENTS AND A CONFIRMATION OF GENETIC STABILITY WITH SCOT AND RAPD MARKERS

Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Ch...

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Main Authors: Justyna Lema-Rumińska, Natalia Miler, Krzysztof Gęsiński
Format: Article
Language:English
Published: University of Life Sciences in Lublin - Publishing House 2018-02-01
Series:Acta Scientiarum Polonorum: Hortorum Cultus
Subjects:
Online Access:https://czasopisma.up.lublin.pl/index.php/asphc/article/view/374
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author Justyna Lema-Rumińska
Natalia Miler
Krzysztof Gęsiński
author_facet Justyna Lema-Rumińska
Natalia Miler
Krzysztof Gęsiński
author_sort Justyna Lema-Rumińska
collection DOAJ
description Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of ‘Faro’ and ‘Titicaca’ cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of ‘Faro’ and ‘Titicaca’, while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of ‘Titicaca’ (genetic distnaces from 7.5% to 55.9%) and in a single plant of ‘Faro’(genetic distance 61.2% as indicated by SCoT technique).
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spelling doaj.art-129a14a79a8f411d8678b79790b6d0fb2023-12-12T07:58:28ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052018-02-0117110.24326/asphc.2018.1.7IDENTIFICATION OF NEW POLISH LINES OF CHENOPODIUM QUINOA (WILLD.) BY SPECTRAL ANALYSIS OF PIGMENTS AND A CONFIRMATION OF GENETIC STABILITY WITH SCOT AND RAPD MARKERSJustyna Lema-RumińskaNatalia Miler0Krzysztof Gęsiński1Laboratory of Biotechnology, Department of Ornamental Plants and Vegetable Crops, University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, PolandDepartment of Botany and Ecology, University of Science and Technology, Kaliskiego Ave. 7, 85-789 Bydgoszcz, Poland Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of ‘Faro’ and ‘Titicaca’ cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of ‘Faro’ and ‘Titicaca’, while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of ‘Titicaca’ (genetic distnaces from 7.5% to 55.9%) and in a single plant of ‘Faro’(genetic distance 61.2% as indicated by SCoT technique). https://czasopisma.up.lublin.pl/index.php/asphc/article/view/374breedingmolecular markersquinoapigments
spellingShingle Justyna Lema-Rumińska
Natalia Miler
Krzysztof Gęsiński
IDENTIFICATION OF NEW POLISH LINES OF CHENOPODIUM QUINOA (WILLD.) BY SPECTRAL ANALYSIS OF PIGMENTS AND A CONFIRMATION OF GENETIC STABILITY WITH SCOT AND RAPD MARKERS
Acta Scientiarum Polonorum: Hortorum Cultus
breeding
molecular markers
quinoa
pigments
title IDENTIFICATION OF NEW POLISH LINES OF CHENOPODIUM QUINOA (WILLD.) BY SPECTRAL ANALYSIS OF PIGMENTS AND A CONFIRMATION OF GENETIC STABILITY WITH SCOT AND RAPD MARKERS
title_full IDENTIFICATION OF NEW POLISH LINES OF CHENOPODIUM QUINOA (WILLD.) BY SPECTRAL ANALYSIS OF PIGMENTS AND A CONFIRMATION OF GENETIC STABILITY WITH SCOT AND RAPD MARKERS
title_fullStr IDENTIFICATION OF NEW POLISH LINES OF CHENOPODIUM QUINOA (WILLD.) BY SPECTRAL ANALYSIS OF PIGMENTS AND A CONFIRMATION OF GENETIC STABILITY WITH SCOT AND RAPD MARKERS
title_full_unstemmed IDENTIFICATION OF NEW POLISH LINES OF CHENOPODIUM QUINOA (WILLD.) BY SPECTRAL ANALYSIS OF PIGMENTS AND A CONFIRMATION OF GENETIC STABILITY WITH SCOT AND RAPD MARKERS
title_short IDENTIFICATION OF NEW POLISH LINES OF CHENOPODIUM QUINOA (WILLD.) BY SPECTRAL ANALYSIS OF PIGMENTS AND A CONFIRMATION OF GENETIC STABILITY WITH SCOT AND RAPD MARKERS
title_sort identification of new polish lines of chenopodium quinoa willd by spectral analysis of pigments and a confirmation of genetic stability with scot and rapd markers
topic breeding
molecular markers
quinoa
pigments
url https://czasopisma.up.lublin.pl/index.php/asphc/article/view/374
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AT nataliamiler identificationofnewpolishlinesofchenopodiumquinoawilldbyspectralanalysisofpigmentsandaconfirmationofgeneticstabilitywithscotandrapdmarkers
AT krzysztofgesinski identificationofnewpolishlinesofchenopodiumquinoawilldbyspectralanalysisofpigmentsandaconfirmationofgeneticstabilitywithscotandrapdmarkers