Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health
Abstract Salmonella enterica variants exhibit diverse host adaptation, outcome of infection, and associated risk to food safety. Analysis of the distribution of Salmonella enterica serovar Derby (S. Derby) subtypes in human and swine identified isolates with a distinct PFGE profile that were signifi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-78443-7 |
| _version_ | 1818429992349990912 |
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| author | Martina Tambassi Melissa Berni Chiara Bracchi Erika Scaltriti Marina Morganti Luca Bolzoni Jennifer R. Tanner Gaëtan Thilliez Robert A. Kingsley Stefano Pongolini Gabriele Casadei |
| author_facet | Martina Tambassi Melissa Berni Chiara Bracchi Erika Scaltriti Marina Morganti Luca Bolzoni Jennifer R. Tanner Gaëtan Thilliez Robert A. Kingsley Stefano Pongolini Gabriele Casadei |
| author_sort | Martina Tambassi |
| collection | DOAJ |
| description | Abstract Salmonella enterica variants exhibit diverse host adaptation, outcome of infection, and associated risk to food safety. Analysis of the distribution of Salmonella enterica serovar Derby (S. Derby) subtypes in human and swine identified isolates with a distinct PFGE profile that were significantly under-represented in human infections, consistent with further host adaptation to swine. Here we show that isolates with this PFGE profile form a distinct phylogenetic sub-clade within S. Derby and exhibit a profound reduction in invasion of human epithelial cells, and a relatively small reduction in swine epithelial cells. A single missense mutation in hilD, that encodes the master-regulator of the Salmonella Pathogenicity Island 1 (SPI-1), was present in the adapted lineage. The missense mutation resulted in a loss of function of HilD that accounted for reduced invasion in human epithelial cells. The relatively small impact of the mutation on interaction with swine cells was consistent with an alternative mechanism of invasion in this pathogen-host combination. |
| first_indexed | 2024-12-14T15:26:19Z |
| format | Article |
| id | doaj.art-129ad161b59b4c36a5a84aad6931a6d0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:26:19Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-129ad161b59b4c36a5a84aad6931a6d02022-12-21T22:56:00ZengNature PortfolioScientific Reports2045-23222020-12-0110111310.1038/s41598-020-78443-7Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human healthMartina Tambassi0Melissa Berni1Chiara Bracchi2Erika Scaltriti3Marina Morganti4Luca Bolzoni5Jennifer R. Tanner6Gaëtan Thilliez7Robert A. Kingsley8Stefano Pongolini9Gabriele Casadei10Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaRisk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaRisk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaRisk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaRisk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaRisk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaQuadram Institute BioscienceQuadram Institute BioscienceQuadram Institute BioscienceRisk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaRisk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaAbstract Salmonella enterica variants exhibit diverse host adaptation, outcome of infection, and associated risk to food safety. Analysis of the distribution of Salmonella enterica serovar Derby (S. Derby) subtypes in human and swine identified isolates with a distinct PFGE profile that were significantly under-represented in human infections, consistent with further host adaptation to swine. Here we show that isolates with this PFGE profile form a distinct phylogenetic sub-clade within S. Derby and exhibit a profound reduction in invasion of human epithelial cells, and a relatively small reduction in swine epithelial cells. A single missense mutation in hilD, that encodes the master-regulator of the Salmonella Pathogenicity Island 1 (SPI-1), was present in the adapted lineage. The missense mutation resulted in a loss of function of HilD that accounted for reduced invasion in human epithelial cells. The relatively small impact of the mutation on interaction with swine cells was consistent with an alternative mechanism of invasion in this pathogen-host combination.https://doi.org/10.1038/s41598-020-78443-7 |
| spellingShingle | Martina Tambassi Melissa Berni Chiara Bracchi Erika Scaltriti Marina Morganti Luca Bolzoni Jennifer R. Tanner Gaëtan Thilliez Robert A. Kingsley Stefano Pongolini Gabriele Casadei Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health Scientific Reports |
| title | Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health |
| title_full | Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health |
| title_fullStr | Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health |
| title_full_unstemmed | Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health |
| title_short | Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health |
| title_sort | mutation of hild in a salmonella derby lineage linked to swine adaptation and reduced risk to human health |
| url | https://doi.org/10.1038/s41598-020-78443-7 |
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