Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics
Summary: T cells become activated following one or multiple contacts with antigen-presenting cells. Calcium influx is a key signaling event elicited during these cellular interactions; however, it is unclear whether T cells recall and integrate calcium signals elicited during temporally separated co...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223001451 |
| _version_ | 1797903291207647232 |
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| author | Béatrice Corre Yassine El Janati Elidrissi Justine Duval Mailys Quilhot Gaëtan Lefebvre Solène Ecomard Fabrice Lemaître Zacarias Garcia Armelle Bohineust Erica Russo Philippe Bousso |
| author_facet | Béatrice Corre Yassine El Janati Elidrissi Justine Duval Mailys Quilhot Gaëtan Lefebvre Solène Ecomard Fabrice Lemaître Zacarias Garcia Armelle Bohineust Erica Russo Philippe Bousso |
| author_sort | Béatrice Corre |
| collection | DOAJ |
| description | Summary: T cells become activated following one or multiple contacts with antigen-presenting cells. Calcium influx is a key signaling event elicited during these cellular interactions; however, it is unclear whether T cells recall and integrate calcium signals elicited during temporally separated contacts. To study the integration of calcium signals, we designed a programmable, multiplex illumination strategy for temporally patterned optogenetics (TEMPO). We found that a single round of calcium elevation was insufficient to promote nuclear factor of activated T cells (NFAT) activity and cytokine production in a T cell line. However, robust responses were detected after a second identical stimulation even when signals were separated by several hours. Our results suggest the existence of a biochemical memory of calcium signals in T cells that favors signal integration during temporally separated contacts and promote cytokine production. As illustrated here, TEMPO is a versatile approach for dissecting temporal integration in defined signaling pathways. |
| first_indexed | 2024-04-10T09:30:33Z |
| format | Article |
| id | doaj.art-129b59cdef164681884d2a17a20e5701 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T09:30:33Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-129b59cdef164681884d2a17a20e57012023-02-19T04:27:08ZengElsevieriScience2589-00422023-02-01262106068Integration of intermittent calcium signals in T cells revealed by temporally patterned optogeneticsBéatrice Corre0Yassine El Janati Elidrissi1Justine Duval2Mailys Quilhot3Gaëtan Lefebvre4Solène Ecomard5Fabrice Lemaître6Zacarias Garcia7Armelle Bohineust8Erica Russo9Philippe Bousso10Dynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, FranceDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France; Corresponding authorSummary: T cells become activated following one or multiple contacts with antigen-presenting cells. Calcium influx is a key signaling event elicited during these cellular interactions; however, it is unclear whether T cells recall and integrate calcium signals elicited during temporally separated contacts. To study the integration of calcium signals, we designed a programmable, multiplex illumination strategy for temporally patterned optogenetics (TEMPO). We found that a single round of calcium elevation was insufficient to promote nuclear factor of activated T cells (NFAT) activity and cytokine production in a T cell line. However, robust responses were detected after a second identical stimulation even when signals were separated by several hours. Our results suggest the existence of a biochemical memory of calcium signals in T cells that favors signal integration during temporally separated contacts and promote cytokine production. As illustrated here, TEMPO is a versatile approach for dissecting temporal integration in defined signaling pathways.http://www.sciencedirect.com/science/article/pii/S2589004223001451Biological sciencesBiochemistryImmunologyImmunological methods |
| spellingShingle | Béatrice Corre Yassine El Janati Elidrissi Justine Duval Mailys Quilhot Gaëtan Lefebvre Solène Ecomard Fabrice Lemaître Zacarias Garcia Armelle Bohineust Erica Russo Philippe Bousso Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics iScience Biological sciences Biochemistry Immunology Immunological methods |
| title | Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics |
| title_full | Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics |
| title_fullStr | Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics |
| title_full_unstemmed | Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics |
| title_short | Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics |
| title_sort | integration of intermittent calcium signals in t cells revealed by temporally patterned optogenetics |
| topic | Biological sciences Biochemistry Immunology Immunological methods |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223001451 |
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