Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane
Purpose: To develop a viable in vivo chorioallantoic membrane (CAM) model to study the growth and invasion of patient-derived retinoblastoma (RB) and choroidal melanoma (CM) xenografts (PDXs). The study utilizes primary tumor samples instead of cancer cell lines, which provides a more authentic repr...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Indian Journal of Ophthalmology |
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| Online Access: | http://www.ijo.in/article.asp?issn=0301-4738;year=2023;volume=71;issue=3;spage=977;epage=982;aulast=Kant |
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| author | Nimita Kant Perumal Jayaraj Seema Sen Harshita Rupani Pranavi Kumar Shefali Dahiya Palak Chugh Muskaan Gupta Manisha Sengar |
| author_facet | Nimita Kant Perumal Jayaraj Seema Sen Harshita Rupani Pranavi Kumar Shefali Dahiya Palak Chugh Muskaan Gupta Manisha Sengar |
| author_sort | Nimita Kant |
| collection | DOAJ |
| description | Purpose: To develop a viable in vivo chorioallantoic membrane (CAM) model to study the growth and invasion of patient-derived retinoblastoma (RB) and choroidal melanoma (CM) xenografts (PDXs). The study utilizes primary tumor samples instead of cancer cell lines, which provides a more authentic representation of tumors due to conserved morphology and heterogeneity. Methods: Fertilized chicken eggs were procured, windowed, and their CAM layers were dropped. On embryonic development day (EDD) 10, freshly cut patient-derived CM and RB tumors were implanted on the CAM layer and the setup was incubated for 7 days. The tumor-embedded CAM layer was harvested on EDD 17, and the extracted tumor samples were subjected to hematoxylin and eosin staining and immunohistochemical analysis to evaluate the extent of tumor invasion. Results: Significant changes in the vascularity around the RB and CM PDXs were observed, indicating an angiogenic environment. The cross-sectional histological view of the tumor implant site revealed the invasion of both the tumors into the CAM mesoderm. Invasion of CM into CAM mesoderm was visualized in the form of pigmented nodules, and that of RB was indicated by synaptophysin and Ki-67 positivity in Immunohistochemistry (IHC). Conclusion: The CAM xenograft model was successfully able to support the growth of CM and RB PDXs and their invasion in CAM, thus presenting as a feasible alternative to mammalian models for studying tumorigenicity and invasiveness of ocular tumors. Moreover, this model can further be utilized to develop personalized medicine by inoculating patient-specific tumors for preclinical drug screening. |
| first_indexed | 2024-03-13T10:35:41Z |
| format | Article |
| id | doaj.art-129bdc56aece4ebea85d3d13008e2bf7 |
| institution | Directory Open Access Journal |
| issn | 0301-4738 1998-3689 |
| language | English |
| last_indexed | 2024-03-13T10:35:41Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Ophthalmology |
| spelling | doaj.art-129bdc56aece4ebea85d3d13008e2bf72023-05-18T06:11:44ZengWolters Kluwer Medknow PublicationsIndian Journal of Ophthalmology0301-47381998-36892023-01-0171397798210.4103/ijo.IJO_1494_22Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membraneNimita KantPerumal JayarajSeema SenHarshita RupaniPranavi KumarShefali DahiyaPalak ChughMuskaan GuptaManisha SengarPurpose: To develop a viable in vivo chorioallantoic membrane (CAM) model to study the growth and invasion of patient-derived retinoblastoma (RB) and choroidal melanoma (CM) xenografts (PDXs). The study utilizes primary tumor samples instead of cancer cell lines, which provides a more authentic representation of tumors due to conserved morphology and heterogeneity. Methods: Fertilized chicken eggs were procured, windowed, and their CAM layers were dropped. On embryonic development day (EDD) 10, freshly cut patient-derived CM and RB tumors were implanted on the CAM layer and the setup was incubated for 7 days. The tumor-embedded CAM layer was harvested on EDD 17, and the extracted tumor samples were subjected to hematoxylin and eosin staining and immunohistochemical analysis to evaluate the extent of tumor invasion. Results: Significant changes in the vascularity around the RB and CM PDXs were observed, indicating an angiogenic environment. The cross-sectional histological view of the tumor implant site revealed the invasion of both the tumors into the CAM mesoderm. Invasion of CM into CAM mesoderm was visualized in the form of pigmented nodules, and that of RB was indicated by synaptophysin and Ki-67 positivity in Immunohistochemistry (IHC). Conclusion: The CAM xenograft model was successfully able to support the growth of CM and RB PDXs and their invasion in CAM, thus presenting as a feasible alternative to mammalian models for studying tumorigenicity and invasiveness of ocular tumors. Moreover, this model can further be utilized to develop personalized medicine by inoculating patient-specific tumors for preclinical drug screening.http://www.ijo.in/article.asp?issn=0301-4738;year=2023;volume=71;issue=3;spage=977;epage=982;aulast=Kantcamchickenchorioallantoic membranechoroidal melanomaeye cancerocular cancerpatient-derived xenograftretinoblastomauveal melanoma |
| spellingShingle | Nimita Kant Perumal Jayaraj Seema Sen Harshita Rupani Pranavi Kumar Shefali Dahiya Palak Chugh Muskaan Gupta Manisha Sengar Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane Indian Journal of Ophthalmology cam chicken chorioallantoic membrane choroidal melanoma eye cancer ocular cancer patient-derived xenograft retinoblastoma uveal melanoma |
| title | Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane |
| title_full | Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane |
| title_fullStr | Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane |
| title_full_unstemmed | Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane |
| title_short | Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane |
| title_sort | establishment of patient derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane |
| topic | cam chicken chorioallantoic membrane choroidal melanoma eye cancer ocular cancer patient-derived xenograft retinoblastoma uveal melanoma |
| url | http://www.ijo.in/article.asp?issn=0301-4738;year=2023;volume=71;issue=3;spage=977;epage=982;aulast=Kant |
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