Triploid Hybrids of 2<i>x</i> Lingonberry (<i>Vaccinium vitis-idaea</i>) by 2<i>x</i> Black Highbush Blueberry (<i>V. fuscatum</i>) and 2<i>x</i> Elliott’s Blueberry (<i>V. elliottii</i>) as Evidence of a Genome Balance Requirement for Hybridization Success
Hybridizations were made between a 2<i>x V. vitis-idaea</i> (sect. <i>Vitis-idaea</i>, lingonberry) and 2<i>x V. fuscatum</i> (sect. <i>Cyanococcus</i>, Black Highbush Blueberry) as part of a project aimed at understanding the crossability and compatib...
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MDPI AG
2023-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/12/1308 |
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| author | Mark Ehlenfeldt James L. Luteyn Fernando de la Torre Juan Zalapa |
| author_facet | Mark Ehlenfeldt James L. Luteyn Fernando de la Torre Juan Zalapa |
| author_sort | Mark Ehlenfeldt |
| collection | DOAJ |
| description | Hybridizations were made between a 2<i>x V. vitis-idaea</i> (sect. <i>Vitis-idaea</i>, lingonberry) and 2<i>x V. fuscatum</i> (sect. <i>Cyanococcus</i>, Black Highbush Blueberry) as part of a project aimed at understanding the crossability and compatibility of these but specifically aimed at assessing the possibilities for improvement and utilization of lingonberry. The crosses succeeded at a low level, and six hybrids were produced and genetically verified. When tested for ploidy level, five of the six hybrids were found to be triploids and one was found to be a tetraploid. Hybrids were intermediate in morphology and only fertile at very low levels, largely due to their triploid nature. Several of these hybrids produced progeny when used as females and pollinated with 6<i>x V. virgatum</i>-derived males, indicating viable unreduced 2<i>n</i> ovules. Similar crosses were made between lingonberry and 2<i>x V. elliottii</i> (sect. <i>Cyanococcus</i>, Elliott’s Blueberry). These crosses produced two genetically verified hybrids, which were also determined to be triploids. These hybrids were effectively sterile. The production of triploids from 2<i>x</i> × 2<i>x</i> crosses indicates that there is a natural selection for a reproductive genome balance of two <i>V. vitis-idaea</i>:one <i>Cyanococcus</i>. The success of secondary hybridizations with hexaploid materials suggests that the triploid hybrids may be used to advance the utilization and recombination of lingonberry germplasm. |
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| format | Article |
| id | doaj.art-129d3c6f81c54a989e7284805f070c7c |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-08T20:42:26Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-129d3c6f81c54a989e7284805f070c7c2023-12-22T14:12:34ZengMDPI AGHorticulturae2311-75242023-12-01912130810.3390/horticulturae9121308Triploid Hybrids of 2<i>x</i> Lingonberry (<i>Vaccinium vitis-idaea</i>) by 2<i>x</i> Black Highbush Blueberry (<i>V. fuscatum</i>) and 2<i>x</i> Elliott’s Blueberry (<i>V. elliottii</i>) as Evidence of a Genome Balance Requirement for Hybridization SuccessMark Ehlenfeldt0James L. Luteyn1Fernando de la Torre2Juan Zalapa3USDA-ARS, Philip E. Marucci Center for Blueberry and Cranberry Research and Extension, Chatsworth, NJ 08019, USANew York Botanical Garden, Bronx, NY 10458, USADepartment of Plant and Agroecosystem Sciences, University of Wisconsin-Madison, Madison, WI 53706, USADepartment of Plant and Agroecosystem Sciences, University of Wisconsin-Madison, Madison, WI 53706, USAHybridizations were made between a 2<i>x V. vitis-idaea</i> (sect. <i>Vitis-idaea</i>, lingonberry) and 2<i>x V. fuscatum</i> (sect. <i>Cyanococcus</i>, Black Highbush Blueberry) as part of a project aimed at understanding the crossability and compatibility of these but specifically aimed at assessing the possibilities for improvement and utilization of lingonberry. The crosses succeeded at a low level, and six hybrids were produced and genetically verified. When tested for ploidy level, five of the six hybrids were found to be triploids and one was found to be a tetraploid. Hybrids were intermediate in morphology and only fertile at very low levels, largely due to their triploid nature. Several of these hybrids produced progeny when used as females and pollinated with 6<i>x V. virgatum</i>-derived males, indicating viable unreduced 2<i>n</i> ovules. Similar crosses were made between lingonberry and 2<i>x V. elliottii</i> (sect. <i>Cyanococcus</i>, Elliott’s Blueberry). These crosses produced two genetically verified hybrids, which were also determined to be triploids. These hybrids were effectively sterile. The production of triploids from 2<i>x</i> × 2<i>x</i> crosses indicates that there is a natural selection for a reproductive genome balance of two <i>V. vitis-idaea</i>:one <i>Cyanococcus</i>. The success of secondary hybridizations with hexaploid materials suggests that the triploid hybrids may be used to advance the utilization and recombination of lingonberry germplasm.https://www.mdpi.com/2311-7524/9/12/1308<i>V. corymbosum</i> forma <i>atrococcum</i>intersectional hybridgenome strengthEBN (Endosperm Balance Number) |
| spellingShingle | Mark Ehlenfeldt James L. Luteyn Fernando de la Torre Juan Zalapa Triploid Hybrids of 2<i>x</i> Lingonberry (<i>Vaccinium vitis-idaea</i>) by 2<i>x</i> Black Highbush Blueberry (<i>V. fuscatum</i>) and 2<i>x</i> Elliott’s Blueberry (<i>V. elliottii</i>) as Evidence of a Genome Balance Requirement for Hybridization Success Horticulturae <i>V. corymbosum</i> forma <i>atrococcum</i> intersectional hybrid genome strength EBN (Endosperm Balance Number) |
| title | Triploid Hybrids of 2<i>x</i> Lingonberry (<i>Vaccinium vitis-idaea</i>) by 2<i>x</i> Black Highbush Blueberry (<i>V. fuscatum</i>) and 2<i>x</i> Elliott’s Blueberry (<i>V. elliottii</i>) as Evidence of a Genome Balance Requirement for Hybridization Success |
| title_full | Triploid Hybrids of 2<i>x</i> Lingonberry (<i>Vaccinium vitis-idaea</i>) by 2<i>x</i> Black Highbush Blueberry (<i>V. fuscatum</i>) and 2<i>x</i> Elliott’s Blueberry (<i>V. elliottii</i>) as Evidence of a Genome Balance Requirement for Hybridization Success |
| title_fullStr | Triploid Hybrids of 2<i>x</i> Lingonberry (<i>Vaccinium vitis-idaea</i>) by 2<i>x</i> Black Highbush Blueberry (<i>V. fuscatum</i>) and 2<i>x</i> Elliott’s Blueberry (<i>V. elliottii</i>) as Evidence of a Genome Balance Requirement for Hybridization Success |
| title_full_unstemmed | Triploid Hybrids of 2<i>x</i> Lingonberry (<i>Vaccinium vitis-idaea</i>) by 2<i>x</i> Black Highbush Blueberry (<i>V. fuscatum</i>) and 2<i>x</i> Elliott’s Blueberry (<i>V. elliottii</i>) as Evidence of a Genome Balance Requirement for Hybridization Success |
| title_short | Triploid Hybrids of 2<i>x</i> Lingonberry (<i>Vaccinium vitis-idaea</i>) by 2<i>x</i> Black Highbush Blueberry (<i>V. fuscatum</i>) and 2<i>x</i> Elliott’s Blueberry (<i>V. elliottii</i>) as Evidence of a Genome Balance Requirement for Hybridization Success |
| title_sort | triploid hybrids of 2 i x i lingonberry i vaccinium vitis idaea i by 2 i x i black highbush blueberry i v fuscatum i and 2 i x i elliott s blueberry i v elliottii i as evidence of a genome balance requirement for hybridization success |
| topic | <i>V. corymbosum</i> forma <i>atrococcum</i> intersectional hybrid genome strength EBN (Endosperm Balance Number) |
| url | https://www.mdpi.com/2311-7524/9/12/1308 |
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