The Influence of Different Forms of Silver on Selected Pathogenic Bacteria
The application of silver nanoparticles as an antibacterial agent is becoming more common. Unfortunately, their effect on microorganisms is still not fully understood. Therefore, this paper attempts to investigate the influence of silver ions, biologically synthesized silver nanoparticles and nanopa...
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MDPI AG
2020-05-01
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| Online Access: | https://www.mdpi.com/1996-1944/13/10/2403 |
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| author | Bogusław Buszewski Agnieszka Rogowska Viorica Railean-Plugaru Michał Złoch Justyna Walczak-Skierska Paweł Pomastowski |
| author_facet | Bogusław Buszewski Agnieszka Rogowska Viorica Railean-Plugaru Michał Złoch Justyna Walczak-Skierska Paweł Pomastowski |
| author_sort | Bogusław Buszewski |
| collection | DOAJ |
| description | The application of silver nanoparticles as an antibacterial agent is becoming more common. Unfortunately, their effect on microorganisms is still not fully understood. Therefore, this paper attempts to investigate the influence of silver ions, biologically synthesized silver nanoparticles and nanoparticles functionalized with antibiotics on molecular bacteria profiles. The initial stage of research was aimed at the mechanism determination involved in antibiotics sorption onto nanoparticles’ surface. For this purpose, the kinetics study was performed. Next, the functionalized formulations were characterized by Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS) and a zeta potential study. The results reveal that functionalization is a complex process, but does not significantly affect the stability of biocolloids. Furthermore, the antimicrobial assays, in most cases, have shown no increases in antibacterial activity after nanoparticle functionalization, which suggests that the functionalization process does not always generate the improved antimicrobial effect. Finally, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique was employed to characterize the changes in the molecular profile of bacteria treated with various antibacterial agents. The recorded spectra proved many differences in bacterial lipids and proteins profiles compared to untreated cells. In addition, the statistical analysis of recorded spectra revealed the strain-dependent nature of stress factors on the molecular profile of microorganisms. |
| first_indexed | 2024-03-10T19:38:19Z |
| format | Article |
| id | doaj.art-129e24ce351b4fe99088291225b58b85 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T19:38:19Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
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| series | Materials |
| spelling | doaj.art-129e24ce351b4fe99088291225b58b852023-11-20T01:28:51ZengMDPI AGMaterials1996-19442020-05-011310240310.3390/ma13102403The Influence of Different Forms of Silver on Selected Pathogenic BacteriaBogusław Buszewski0Agnieszka Rogowska1Viorica Railean-Plugaru2Michał Złoch3Justyna Walczak-Skierska4Paweł Pomastowski5Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wileńska 4, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wileńska 4, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wileńska 4, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wileńska 4, 87-100 Torun, PolandDepartment of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wileńska 4, 87-100 Torun, PolandThe application of silver nanoparticles as an antibacterial agent is becoming more common. Unfortunately, their effect on microorganisms is still not fully understood. Therefore, this paper attempts to investigate the influence of silver ions, biologically synthesized silver nanoparticles and nanoparticles functionalized with antibiotics on molecular bacteria profiles. The initial stage of research was aimed at the mechanism determination involved in antibiotics sorption onto nanoparticles’ surface. For this purpose, the kinetics study was performed. Next, the functionalized formulations were characterized by Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS) and a zeta potential study. The results reveal that functionalization is a complex process, but does not significantly affect the stability of biocolloids. Furthermore, the antimicrobial assays, in most cases, have shown no increases in antibacterial activity after nanoparticle functionalization, which suggests that the functionalization process does not always generate the improved antimicrobial effect. Finally, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique was employed to characterize the changes in the molecular profile of bacteria treated with various antibacterial agents. The recorded spectra proved many differences in bacterial lipids and proteins profiles compared to untreated cells. In addition, the statistical analysis of recorded spectra revealed the strain-dependent nature of stress factors on the molecular profile of microorganisms.https://www.mdpi.com/1996-1944/13/10/2403silver nanocompositesfunctionalizationkineticMALDI-TOF MSbacteria molecular profile |
| spellingShingle | Bogusław Buszewski Agnieszka Rogowska Viorica Railean-Plugaru Michał Złoch Justyna Walczak-Skierska Paweł Pomastowski The Influence of Different Forms of Silver on Selected Pathogenic Bacteria Materials silver nanocomposites functionalization kinetic MALDI-TOF MS bacteria molecular profile |
| title | The Influence of Different Forms of Silver on Selected Pathogenic Bacteria |
| title_full | The Influence of Different Forms of Silver on Selected Pathogenic Bacteria |
| title_fullStr | The Influence of Different Forms of Silver on Selected Pathogenic Bacteria |
| title_full_unstemmed | The Influence of Different Forms of Silver on Selected Pathogenic Bacteria |
| title_short | The Influence of Different Forms of Silver on Selected Pathogenic Bacteria |
| title_sort | influence of different forms of silver on selected pathogenic bacteria |
| topic | silver nanocomposites functionalization kinetic MALDI-TOF MS bacteria molecular profile |
| url | https://www.mdpi.com/1996-1944/13/10/2403 |
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