<i>Pou5f1</i> and <i>Nanog</i> Are Reliable Germ Cell-Specific Genes in Gonad of a Protogynous Hermaphroditic Fish, Orange-Spotted Grouper (<i>Epinephelus coioides</i>)
Pluripotency markers Pou5f1 and Nanog are core transcription factors regulating early embryonic development and maintaining the pluripotency and self-renewal of stem cells. Pou5f1 and Nanog also play important roles in germ cell development and gametogenesis. In this study, <i>Pou5f1</i>...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/1/79 |
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| author | Chaoyue Zhong Meifeng Liu Yuhao Tao Xi Wu Yang Yang Tong Wang Zining Meng Hongyan Xu Xiaochun Liu |
| author_facet | Chaoyue Zhong Meifeng Liu Yuhao Tao Xi Wu Yang Yang Tong Wang Zining Meng Hongyan Xu Xiaochun Liu |
| author_sort | Chaoyue Zhong |
| collection | DOAJ |
| description | Pluripotency markers Pou5f1 and Nanog are core transcription factors regulating early embryonic development and maintaining the pluripotency and self-renewal of stem cells. Pou5f1 and Nanog also play important roles in germ cell development and gametogenesis. In this study, <i>Pou5f1</i> (<i>EcPou5f1</i>) and <i>Nanog</i> (<i>EcNanog</i>) were cloned from orange-spotted grouper, <i>Epinephelus coioides</i>. The full-length cDNAs of <i>EcPou5f1</i> and <i>EcNanog</i> were 2790 and 1820 bp, and encoded 475 and 432 amino acids, respectively. <i>EcPou5f1</i> exhibited a specific expression in gonads, whereas <i>EcNanog</i> was expressed highly in gonads and weakly in some somatic tissues. In situ hybridization analyses showed that the mRNA signals of <i>EcNanog</i> and <i>EcPou5f1</i> were exclusively restricted to germ cells in gonads. Likewise, immunohistofluorescence staining revealed that EcNanog protein was limited to germ cells. Moreover, both <i>EcPou5f1</i> and <i>EcNanog</i> mRNAs were discovered to be co-localized with <i>Vasa</i> mRNA, a well-known germ cell maker, in male and female germ cells. These results implied that <i>EcPou5f1</i> and <i>EcNanog</i> could be also regarded as reliable germ cell marker genes. Therefore, the findings of this study would pave the way for elucidating the mechanism whereby <i>EcPou5f1</i> and <i>EcNanog</i> regulate germ cell development and gametogenesis in grouper fish, and even in other protogynous hermaphroditic species. |
| first_indexed | 2024-03-10T01:25:29Z |
| format | Article |
| id | doaj.art-129e6c8a010b42bc9ea859c54e887463 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T01:25:29Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-129e6c8a010b42bc9ea859c54e8874632023-11-23T13:52:03ZengMDPI AGGenes2073-44252021-12-011317910.3390/genes13010079<i>Pou5f1</i> and <i>Nanog</i> Are Reliable Germ Cell-Specific Genes in Gonad of a Protogynous Hermaphroditic Fish, Orange-Spotted Grouper (<i>Epinephelus coioides</i>)Chaoyue Zhong0Meifeng Liu1Yuhao Tao2Xi Wu3Yang Yang4Tong Wang5Zining Meng6Hongyan Xu7Xiaochun Liu8State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Sciences of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaState Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaPluripotency markers Pou5f1 and Nanog are core transcription factors regulating early embryonic development and maintaining the pluripotency and self-renewal of stem cells. Pou5f1 and Nanog also play important roles in germ cell development and gametogenesis. In this study, <i>Pou5f1</i> (<i>EcPou5f1</i>) and <i>Nanog</i> (<i>EcNanog</i>) were cloned from orange-spotted grouper, <i>Epinephelus coioides</i>. The full-length cDNAs of <i>EcPou5f1</i> and <i>EcNanog</i> were 2790 and 1820 bp, and encoded 475 and 432 amino acids, respectively. <i>EcPou5f1</i> exhibited a specific expression in gonads, whereas <i>EcNanog</i> was expressed highly in gonads and weakly in some somatic tissues. In situ hybridization analyses showed that the mRNA signals of <i>EcNanog</i> and <i>EcPou5f1</i> were exclusively restricted to germ cells in gonads. Likewise, immunohistofluorescence staining revealed that EcNanog protein was limited to germ cells. Moreover, both <i>EcPou5f1</i> and <i>EcNanog</i> mRNAs were discovered to be co-localized with <i>Vasa</i> mRNA, a well-known germ cell maker, in male and female germ cells. These results implied that <i>EcPou5f1</i> and <i>EcNanog</i> could be also regarded as reliable germ cell marker genes. Therefore, the findings of this study would pave the way for elucidating the mechanism whereby <i>EcPou5f1</i> and <i>EcNanog</i> regulate germ cell development and gametogenesis in grouper fish, and even in other protogynous hermaphroditic species.https://www.mdpi.com/2073-4425/13/1/79<i>Pou5f1</i><i>Nanog</i>germ cellprotogynous hermaphroditic fishorange-spotted grouper |
| spellingShingle | Chaoyue Zhong Meifeng Liu Yuhao Tao Xi Wu Yang Yang Tong Wang Zining Meng Hongyan Xu Xiaochun Liu <i>Pou5f1</i> and <i>Nanog</i> Are Reliable Germ Cell-Specific Genes in Gonad of a Protogynous Hermaphroditic Fish, Orange-Spotted Grouper (<i>Epinephelus coioides</i>) Genes <i>Pou5f1</i> <i>Nanog</i> germ cell protogynous hermaphroditic fish orange-spotted grouper |
| title | <i>Pou5f1</i> and <i>Nanog</i> Are Reliable Germ Cell-Specific Genes in Gonad of a Protogynous Hermaphroditic Fish, Orange-Spotted Grouper (<i>Epinephelus coioides</i>) |
| title_full | <i>Pou5f1</i> and <i>Nanog</i> Are Reliable Germ Cell-Specific Genes in Gonad of a Protogynous Hermaphroditic Fish, Orange-Spotted Grouper (<i>Epinephelus coioides</i>) |
| title_fullStr | <i>Pou5f1</i> and <i>Nanog</i> Are Reliable Germ Cell-Specific Genes in Gonad of a Protogynous Hermaphroditic Fish, Orange-Spotted Grouper (<i>Epinephelus coioides</i>) |
| title_full_unstemmed | <i>Pou5f1</i> and <i>Nanog</i> Are Reliable Germ Cell-Specific Genes in Gonad of a Protogynous Hermaphroditic Fish, Orange-Spotted Grouper (<i>Epinephelus coioides</i>) |
| title_short | <i>Pou5f1</i> and <i>Nanog</i> Are Reliable Germ Cell-Specific Genes in Gonad of a Protogynous Hermaphroditic Fish, Orange-Spotted Grouper (<i>Epinephelus coioides</i>) |
| title_sort | i pou5f1 i and i nanog i are reliable germ cell specific genes in gonad of a protogynous hermaphroditic fish orange spotted grouper i epinephelus coioides i |
| topic | <i>Pou5f1</i> <i>Nanog</i> germ cell protogynous hermaphroditic fish orange-spotted grouper |
| url | https://www.mdpi.com/2073-4425/13/1/79 |
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