Antibodies against SARS-CoV-2 after natural infection in healthcare workers and clinical characteristics as putative antibody production prediction
Introduction: There is a need for detailed data on early antibody responses against SARS-CoV-2 as this may contribute to the prediction of the clinical course of COVID-19 and the optimization of convalescent plasma treatment. This study aims to gain insight into developing antibodies to SARS-CoV-2 i...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Journal of Clinical Virology Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266703802200028X |
| _version_ | 1811340001280000000 |
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| author | D.A.T. Hanssen J. Penders K. Heijgele S. de Leede M. Mulder L.E.A. Bank M.H.C. Slaats P.H.M. Savelkoul I.H.M. van Loo |
| author_facet | D.A.T. Hanssen J. Penders K. Heijgele S. de Leede M. Mulder L.E.A. Bank M.H.C. Slaats P.H.M. Savelkoul I.H.M. van Loo |
| author_sort | D.A.T. Hanssen |
| collection | DOAJ |
| description | Introduction: There is a need for detailed data on early antibody responses against SARS-CoV-2 as this may contribute to the prediction of the clinical course of COVID-19 and the optimization of convalescent plasma treatment. This study aims to gain insight into developing antibodies to SARS-CoV-2 in health care workers (HCWs) infected in the first wave of the SARS-CoV-2 pandemic in the Netherlands. Materials and methods: In this retrospective analysis, sera from PCR-confirmed COVID-19 positive HCWs are included at the time of the initial PCR (T = 0, n = 95) and at least 21 days after the initial serum (T ≥ 21, n = 133). This study assesses correlations between qualitative total Ig, IgM, IgA, IgG, and quantitative anti-S-RBD antibody responses and participant characteristics. Results: Higher Ct values were associated with higher antibody positivity rates for total Ig (OR 1.261 (95% CI 1.095–1.452)), IgM (OR 1.373 (95% CI 1.125–1.675)), and IgA (OR 1.222 (95% CI 1.013–1.475)). Gender was predictive of IgM and IgA antibody positivity rates at T = 0 (OR 0.018 (95% CI 0.001–0.268)) and (OR 0.070 (95% CI 0.008–0.646)). At T ≥ 21, a substantial proportion of HCWs developed IgM (103/133; 77.4%) and total Ig (128/133; 96.2%) antibodies. IgA and IgG seroconversions were observed in only 51.1% (67/131) and 55.7% (73/131) of HCWs. Anti-S-RBD responses were higher when the interval between onset of symptoms and sampling was longer. Conclusion: The findings of this study give insight into early antibody responses and may have implications for the selection of convalescent plasma donors and the further development of monoclonal antibody treatment. |
| first_indexed | 2024-04-13T18:34:50Z |
| format | Article |
| id | doaj.art-129eb5a62a594d5aa465f1996ea67bf8 |
| institution | Directory Open Access Journal |
| issn | 2667-0380 |
| language | English |
| last_indexed | 2024-04-13T18:34:50Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Clinical Virology Plus |
| spelling | doaj.art-129eb5a62a594d5aa465f1996ea67bf82022-12-22T02:34:55ZengElsevierJournal of Clinical Virology Plus2667-03802022-08-0123100089Antibodies against SARS-CoV-2 after natural infection in healthcare workers and clinical characteristics as putative antibody production predictionD.A.T. Hanssen0J. Penders1K. Heijgele2S. de Leede3M. Mulder4L.E.A. Bank5M.H.C. Slaats6P.H.M. Savelkoul7I.H.M. van Loo8Department of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the Netherlands; Care and Primary Health Research Institute (CAPHRI), Maastricht University, the NetherlandsDepartment of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the Netherlands; Care and Primary Health Research Institute (CAPHRI), Maastricht University, the Netherlands; NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, the NetherlandsDepartment of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the NetherlandsDepartment of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the NetherlandsDepartment of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the NetherlandsDepartment of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the NetherlandsDepartment of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the NetherlandsDepartment of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the Netherlands; Care and Primary Health Research Institute (CAPHRI), Maastricht University, the NetherlandsDepartment of Medical Microbiology, infectious diseases & Infection prevention, Maastricht University Medical Center, the Netherlands; Care and Primary Health Research Institute (CAPHRI), Maastricht University, the Netherlands; Corresponding author at: Department of Medical Microbiology, Maastricht University Medical Center Maastricht. P.Debyelaan 25, 6229HX Maastricht, the Netherlands.Introduction: There is a need for detailed data on early antibody responses against SARS-CoV-2 as this may contribute to the prediction of the clinical course of COVID-19 and the optimization of convalescent plasma treatment. This study aims to gain insight into developing antibodies to SARS-CoV-2 in health care workers (HCWs) infected in the first wave of the SARS-CoV-2 pandemic in the Netherlands. Materials and methods: In this retrospective analysis, sera from PCR-confirmed COVID-19 positive HCWs are included at the time of the initial PCR (T = 0, n = 95) and at least 21 days after the initial serum (T ≥ 21, n = 133). This study assesses correlations between qualitative total Ig, IgM, IgA, IgG, and quantitative anti-S-RBD antibody responses and participant characteristics. Results: Higher Ct values were associated with higher antibody positivity rates for total Ig (OR 1.261 (95% CI 1.095–1.452)), IgM (OR 1.373 (95% CI 1.125–1.675)), and IgA (OR 1.222 (95% CI 1.013–1.475)). Gender was predictive of IgM and IgA antibody positivity rates at T = 0 (OR 0.018 (95% CI 0.001–0.268)) and (OR 0.070 (95% CI 0.008–0.646)). At T ≥ 21, a substantial proportion of HCWs developed IgM (103/133; 77.4%) and total Ig (128/133; 96.2%) antibodies. IgA and IgG seroconversions were observed in only 51.1% (67/131) and 55.7% (73/131) of HCWs. Anti-S-RBD responses were higher when the interval between onset of symptoms and sampling was longer. Conclusion: The findings of this study give insight into early antibody responses and may have implications for the selection of convalescent plasma donors and the further development of monoclonal antibody treatment.http://www.sciencedirect.com/science/article/pii/S266703802200028XSARS-CoV-2SerologyAntibody responseECLIAELISA |
| spellingShingle | D.A.T. Hanssen J. Penders K. Heijgele S. de Leede M. Mulder L.E.A. Bank M.H.C. Slaats P.H.M. Savelkoul I.H.M. van Loo Antibodies against SARS-CoV-2 after natural infection in healthcare workers and clinical characteristics as putative antibody production prediction Journal of Clinical Virology Plus SARS-CoV-2 Serology Antibody response ECLIA ELISA |
| title | Antibodies against SARS-CoV-2 after natural infection in healthcare workers and clinical characteristics as putative antibody production prediction |
| title_full | Antibodies against SARS-CoV-2 after natural infection in healthcare workers and clinical characteristics as putative antibody production prediction |
| title_fullStr | Antibodies against SARS-CoV-2 after natural infection in healthcare workers and clinical characteristics as putative antibody production prediction |
| title_full_unstemmed | Antibodies against SARS-CoV-2 after natural infection in healthcare workers and clinical characteristics as putative antibody production prediction |
| title_short | Antibodies against SARS-CoV-2 after natural infection in healthcare workers and clinical characteristics as putative antibody production prediction |
| title_sort | antibodies against sars cov 2 after natural infection in healthcare workers and clinical characteristics as putative antibody production prediction |
| topic | SARS-CoV-2 Serology Antibody response ECLIA ELISA |
| url | http://www.sciencedirect.com/science/article/pii/S266703802200028X |
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