Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit Stress
This study aimed to explore dark septate endophytes (DSE) that may improve the cultivation of medicinal plants in arid ecosystems. We isolated and identified eight DSE species (Acremonium nepalense, Acrocalymma vagum, Alternaria chartarum, Alternaria chlamydospora, Alternaria longissima, Darksidea a...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.522449/full |
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| author | Chao He Wenquan Wang Wenquan Wang Junling Hou Xianen Li |
| author_facet | Chao He Wenquan Wang Wenquan Wang Junling Hou Xianen Li |
| author_sort | Chao He |
| collection | DOAJ |
| description | This study aimed to explore dark septate endophytes (DSE) that may improve the cultivation of medicinal plants in arid ecosystems. We isolated and identified eight DSE species (Acremonium nepalense, Acrocalymma vagum, Alternaria chartarum, Alternaria chlamydospora, Alternaria longissima, Darksidea alpha, Paraphoma chrysanthemicola, and Preussia terricola) colonizing the roots of wild licorice (Glycyrrhiza uralensis) in the desert areas of northwest China. Moreover, we investigated the osmotic stress tolerance of the DSE using pure culture, along with the performance of licorice plants inoculated with the DSE under drought stress in a growth chamber, respectively. Here, five species were first reported in desert habitats. The osmotic-stress tolerance of DSE species was highly variable, A. chlamydospora and P. terricola increased the total biomass and root biomass of the host plant. All DSE except A. vagum and P. chrysanthemicola increased the glycyrrhizic acid content; all DSE except A. chartarum increased the glycyrrhizin content under drought stress. DSE × watering regimen improved the glycyrrhizic acid content, soil organic matter, and available nitrogen. Structural equation model analysis showed that DSE × watering regimen positively affected soil organic matter, and total biomass, root length, glycyrrhizic acid, and glycyrrhizin (Shapotou site); and positively affected soil organic matter, available phosphorus, and glycyrrhizin (Minqin site); and positively affected the root length (Anxi site). DSE from the Shapotou site accounted for 8.0, 13.0, and 11.3% of the variations in total biomass, root biomass, and active ingredient content; DSE from the Minqin site accounted for 6.6 and 8.3% of the variations in total biomass and root biomass; DSE from the Anxi site accounted for 4.2 and 10.7% of the variations in total biomass and root biomass. DSE × watering regimen displayed a general synergistic effect on plant growth and active ingredient contents. These findings suggested that the DSE–plant interactions were affected by both DSE species and DSE originating habitats. As A. chlamydospora and P. terricola positively affected the total biomass, root biomass, and active ingredient content of host plants under drought stress, they may have important uses as promoters for the cultivation of licorice in dryland agriculture. |
| first_indexed | 2024-12-14T18:21:26Z |
| format | Article |
| id | doaj.art-129f0c93a9954fb1b35bc724275695e1 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-14T18:21:26Z |
| publishDate | 2021-06-01 |
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| spelling | doaj.art-129f0c93a9954fb1b35bc724275695e12022-12-21T22:52:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.522449522449Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit StressChao He0Wenquan Wang1Wenquan Wang2Junling Hou3Xianen Li4Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaThis study aimed to explore dark septate endophytes (DSE) that may improve the cultivation of medicinal plants in arid ecosystems. We isolated and identified eight DSE species (Acremonium nepalense, Acrocalymma vagum, Alternaria chartarum, Alternaria chlamydospora, Alternaria longissima, Darksidea alpha, Paraphoma chrysanthemicola, and Preussia terricola) colonizing the roots of wild licorice (Glycyrrhiza uralensis) in the desert areas of northwest China. Moreover, we investigated the osmotic stress tolerance of the DSE using pure culture, along with the performance of licorice plants inoculated with the DSE under drought stress in a growth chamber, respectively. Here, five species were first reported in desert habitats. The osmotic-stress tolerance of DSE species was highly variable, A. chlamydospora and P. terricola increased the total biomass and root biomass of the host plant. All DSE except A. vagum and P. chrysanthemicola increased the glycyrrhizic acid content; all DSE except A. chartarum increased the glycyrrhizin content under drought stress. DSE × watering regimen improved the glycyrrhizic acid content, soil organic matter, and available nitrogen. Structural equation model analysis showed that DSE × watering regimen positively affected soil organic matter, and total biomass, root length, glycyrrhizic acid, and glycyrrhizin (Shapotou site); and positively affected soil organic matter, available phosphorus, and glycyrrhizin (Minqin site); and positively affected the root length (Anxi site). DSE from the Shapotou site accounted for 8.0, 13.0, and 11.3% of the variations in total biomass, root biomass, and active ingredient content; DSE from the Minqin site accounted for 6.6 and 8.3% of the variations in total biomass and root biomass; DSE from the Anxi site accounted for 4.2 and 10.7% of the variations in total biomass and root biomass. DSE × watering regimen displayed a general synergistic effect on plant growth and active ingredient contents. These findings suggested that the DSE–plant interactions were affected by both DSE species and DSE originating habitats. As A. chlamydospora and P. terricola positively affected the total biomass, root biomass, and active ingredient content of host plants under drought stress, they may have important uses as promoters for the cultivation of licorice in dryland agriculture.https://www.frontiersin.org/articles/10.3389/fmicb.2021.522449/fulllicoricedark septate endophytesdesert ecosystemdrought stressinoculation |
| spellingShingle | Chao He Wenquan Wang Wenquan Wang Junling Hou Xianen Li Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit Stress Frontiers in Microbiology licorice dark septate endophytes desert ecosystem drought stress inoculation |
| title | Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit Stress |
| title_full | Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit Stress |
| title_fullStr | Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit Stress |
| title_full_unstemmed | Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit Stress |
| title_short | Dark Septate Endophytes Isolated From Wild Licorice Roots Grown in the Desert Regions of Northwest China Enhance the Growth of Host Plants Under Water Deficit Stress |
| title_sort | dark septate endophytes isolated from wild licorice roots grown in the desert regions of northwest china enhance the growth of host plants under water deficit stress |
| topic | licorice dark septate endophytes desert ecosystem drought stress inoculation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.522449/full |
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