Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
Sankarathi Balaiya, Ravi K Murthy, Vikram S Brar, Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model.Methods: RGC-5 and...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2010-01-01
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| Series: | Clinical Ophthalmology |
| Online Access: | http://www.dovepress.com/evaluation-of-ultraviolet-light-toxicity-on-cultured-retinal-pigment-e-a3899 |
| _version_ | 1818368165439078400 |
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| author | Sankarathi Balaiya Ravi K Murthy Vikram S Brar et al |
| author_facet | Sankarathi Balaiya Ravi K Murthy Vikram S Brar et al |
| author_sort | Sankarathi Balaiya |
| collection | DOAJ |
| description | Sankarathi Balaiya, Ravi K Murthy, Vikram S Brar, Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model.Methods: RGC-5 and ARPE-19 cells were exposed to different time periods of UVB light: 0, 15, 30, and 45 min. They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 and cytochome C by reverse transcriptase polymerase chain reaction and western blot, respectively.Results: Dose-dependent reduction in cell viability to UVB light was demonstrated with parallel increase in ROS. Increased duration of exposure (>15 minutes), was associated with increased expression of bax and cytochrome C, and absence of bcl-2 expression.Conclusion: UVB light exposure results in cell cytotoxicity. The concomitant generation of ROS and expression of apoptotic markers suggests the role of oxidative stress in UVB-mediated apoptosis in an in vitro model of retinal ganglion and pigment epithelial cells.Keywords: ultraviolet light, retinal pigment epithelium, retinal ganglion cell, reactive oxygen species, cytochrome C |
| first_indexed | 2024-12-13T23:03:37Z |
| format | Article |
| id | doaj.art-12a0f6c3c67e4bc29958438114abf9e4 |
| institution | Directory Open Access Journal |
| issn | 1177-5467 1177-5483 |
| language | English |
| last_indexed | 2024-12-13T23:03:37Z |
| publishDate | 2010-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical Ophthalmology |
| spelling | doaj.art-12a0f6c3c67e4bc29958438114abf9e42022-12-21T23:28:20ZengDove Medical PressClinical Ophthalmology1177-54671177-54832010-01-012010default3339Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cellsSankarathi BalaiyaRavi K MurthyVikram S Braret alSankarathi Balaiya, Ravi K Murthy, Vikram S Brar, Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model.Methods: RGC-5 and ARPE-19 cells were exposed to different time periods of UVB light: 0, 15, 30, and 45 min. They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 and cytochome C by reverse transcriptase polymerase chain reaction and western blot, respectively.Results: Dose-dependent reduction in cell viability to UVB light was demonstrated with parallel increase in ROS. Increased duration of exposure (>15 minutes), was associated with increased expression of bax and cytochrome C, and absence of bcl-2 expression.Conclusion: UVB light exposure results in cell cytotoxicity. The concomitant generation of ROS and expression of apoptotic markers suggests the role of oxidative stress in UVB-mediated apoptosis in an in vitro model of retinal ganglion and pigment epithelial cells.Keywords: ultraviolet light, retinal pigment epithelium, retinal ganglion cell, reactive oxygen species, cytochrome Chttp://www.dovepress.com/evaluation-of-ultraviolet-light-toxicity-on-cultured-retinal-pigment-e-a3899 |
| spellingShingle | Sankarathi Balaiya Ravi K Murthy Vikram S Brar et al Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells Clinical Ophthalmology |
| title | Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells |
| title_full | Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells |
| title_fullStr | Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells |
| title_full_unstemmed | Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells |
| title_short | Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells |
| title_sort | evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells |
| url | http://www.dovepress.com/evaluation-of-ultraviolet-light-toxicity-on-cultured-retinal-pigment-e-a3899 |
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