A Highly Sensitive and Selective Nano-Fluorescent Probe for Ratiometric and Visual Detection of Oxytetracycline Benefiting from Dual Roles of Nitrogen-Doped Carbon Dots
The specific detection of oxytetracycline (OTC) residues is significant for food safety and environmental monitoring. However, rapid specific determination of OTC from various tetracyclines is still challenging due to their similar chemical structures. Here, nitrogen-doped carbon dots (NCDs) with ex...
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MDPI AG
2022-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/23/4306 |
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| author | Huifang Wu Mengqi Xu Yubing Chen Haoliang Zhang Yongjun Shen Yanfeng Tang |
| author_facet | Huifang Wu Mengqi Xu Yubing Chen Haoliang Zhang Yongjun Shen Yanfeng Tang |
| author_sort | Huifang Wu |
| collection | DOAJ |
| description | The specific detection of oxytetracycline (OTC) residues is significant for food safety and environmental monitoring. However, rapid specific determination of OTC from various tetracyclines is still challenging due to their similar chemical structures. Here, nitrogen-doped carbon dots (NCDs) with excitation and pH-dependent optical properties and a high-fluorescence quantum yield were successfully synthesized, which were directly employed to fabricate a dual-response fluorescence probe by self-assembly with Eu<sup>3+</sup> (NCDs/Eu<sup>3+</sup>) for the ratiometric determination of OTC. The addition of OTC into the probe greatly enhances the characteristic emission of Eu<sup>3+</sup> due to the “antenna effect”, and the incorporation of NCDs into the probe further improves the Eu<sup>3+</sup> fluorescence by remarkably weakening the quenching effect caused by H<sub>2</sub>O molecules and efficiently shortening the distance of energy transfer from OTC to Eu<sup>3+</sup>. Meanwhile, the fluorescence of NCDs apparently decreases due to aggregation-caused quenching. The results demonstrate that a ratiometric detection of OTC (0.1–25 µM) with a detection limit of 29 nM based on the double response signals is achieved. Additionally, visual semi-quantitative assay of OTC can be realized with the naked eye under a 365 nm UV lamp according to the fluorescence color change of the as-fabricated probe. This probe exhibits acceptable specificity and anti-interference for OTC assay, holding promise for the fast detection of OTC in real water and milk samples. |
| first_indexed | 2024-03-09T17:37:24Z |
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| id | doaj.art-12a6cb26f58447838f47e3a42dd966ed |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T17:37:24Z |
| publishDate | 2022-12-01 |
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| series | Nanomaterials |
| spelling | doaj.art-12a6cb26f58447838f47e3a42dd966ed2023-11-24T11:48:49ZengMDPI AGNanomaterials2079-49912022-12-011223430610.3390/nano12234306A Highly Sensitive and Selective Nano-Fluorescent Probe for Ratiometric and Visual Detection of Oxytetracycline Benefiting from Dual Roles of Nitrogen-Doped Carbon DotsHuifang Wu0Mengqi Xu1Yubing Chen2Haoliang Zhang3Yongjun Shen4Yanfeng Tang5Nantong Key Lab of Intelligent and New Energy Materials, School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, ChinaNantong Key Lab of Intelligent and New Energy Materials, School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, ChinaNantong Key Lab of Intelligent and New Energy Materials, School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, ChinaNantong Key Lab of Intelligent and New Energy Materials, School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, ChinaNantong Key Lab of Intelligent and New Energy Materials, School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, ChinaNantong Key Lab of Intelligent and New Energy Materials, School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, ChinaThe specific detection of oxytetracycline (OTC) residues is significant for food safety and environmental monitoring. However, rapid specific determination of OTC from various tetracyclines is still challenging due to their similar chemical structures. Here, nitrogen-doped carbon dots (NCDs) with excitation and pH-dependent optical properties and a high-fluorescence quantum yield were successfully synthesized, which were directly employed to fabricate a dual-response fluorescence probe by self-assembly with Eu<sup>3+</sup> (NCDs/Eu<sup>3+</sup>) for the ratiometric determination of OTC. The addition of OTC into the probe greatly enhances the characteristic emission of Eu<sup>3+</sup> due to the “antenna effect”, and the incorporation of NCDs into the probe further improves the Eu<sup>3+</sup> fluorescence by remarkably weakening the quenching effect caused by H<sub>2</sub>O molecules and efficiently shortening the distance of energy transfer from OTC to Eu<sup>3+</sup>. Meanwhile, the fluorescence of NCDs apparently decreases due to aggregation-caused quenching. The results demonstrate that a ratiometric detection of OTC (0.1–25 µM) with a detection limit of 29 nM based on the double response signals is achieved. Additionally, visual semi-quantitative assay of OTC can be realized with the naked eye under a 365 nm UV lamp according to the fluorescence color change of the as-fabricated probe. This probe exhibits acceptable specificity and anti-interference for OTC assay, holding promise for the fast detection of OTC in real water and milk samples.https://www.mdpi.com/2079-4991/12/23/4306oxytetracyclinenitrogen-doped carbon dotslanthanideratiometricvisual analysis |
| spellingShingle | Huifang Wu Mengqi Xu Yubing Chen Haoliang Zhang Yongjun Shen Yanfeng Tang A Highly Sensitive and Selective Nano-Fluorescent Probe for Ratiometric and Visual Detection of Oxytetracycline Benefiting from Dual Roles of Nitrogen-Doped Carbon Dots Nanomaterials oxytetracycline nitrogen-doped carbon dots lanthanide ratiometric visual analysis |
| title | A Highly Sensitive and Selective Nano-Fluorescent Probe for Ratiometric and Visual Detection of Oxytetracycline Benefiting from Dual Roles of Nitrogen-Doped Carbon Dots |
| title_full | A Highly Sensitive and Selective Nano-Fluorescent Probe for Ratiometric and Visual Detection of Oxytetracycline Benefiting from Dual Roles of Nitrogen-Doped Carbon Dots |
| title_fullStr | A Highly Sensitive and Selective Nano-Fluorescent Probe for Ratiometric and Visual Detection of Oxytetracycline Benefiting from Dual Roles of Nitrogen-Doped Carbon Dots |
| title_full_unstemmed | A Highly Sensitive and Selective Nano-Fluorescent Probe for Ratiometric and Visual Detection of Oxytetracycline Benefiting from Dual Roles of Nitrogen-Doped Carbon Dots |
| title_short | A Highly Sensitive and Selective Nano-Fluorescent Probe for Ratiometric and Visual Detection of Oxytetracycline Benefiting from Dual Roles of Nitrogen-Doped Carbon Dots |
| title_sort | highly sensitive and selective nano fluorescent probe for ratiometric and visual detection of oxytetracycline benefiting from dual roles of nitrogen doped carbon dots |
| topic | oxytetracycline nitrogen-doped carbon dots lanthanide ratiometric visual analysis |
| url | https://www.mdpi.com/2079-4991/12/23/4306 |
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