Isolation and Characterization of Mosquito-Associated <i>Spiroplasma cantharicola</i> from <i>Aedes japonicus</i> Collected in Hokkaido, Japan
Species of the genus <i>Spiroplasma</i> are common within arthropods and plants worldwide. Mosquito-associated <i>Spiroplasma</i> spp. have been reported to show pathogenicity toward mosquitoes, which serve as vectors of several infectious diseases that have detrimental effec...
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MDPI AG
2021-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/12/1056 |
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| author | Makoto Shimooka Yoshimi Sakurai Yasukazu Muramatsu Leo Uchida |
| author_facet | Makoto Shimooka Yoshimi Sakurai Yasukazu Muramatsu Leo Uchida |
| author_sort | Makoto Shimooka |
| collection | DOAJ |
| description | Species of the genus <i>Spiroplasma</i> are common within arthropods and plants worldwide. Mosquito-associated <i>Spiroplasma</i> spp. have been reported to show pathogenicity toward mosquitoes, which serve as vectors of several infectious diseases that have detrimental effects on public health. Although <i>Spiroplasma</i> spp. are expected to have potential use as biological vector-control tools, characteristics such as their distribution, host species, and cytopathogenic effects (CPEs) are not well understood. In this study, we isolated a <i>Spiroplasma</i> sp. from a female <i>Aedes japonicus</i> collected in Hokkaido, northern Japan. Phylogenetic analysis based on the 16S rRNA gene sequence indicated our isolate was closely related to <i>S. cantharicola</i>. We screened 103 mosquito pools consisting of 3 genera and 9 species, but only detected <i>S. cantharicola</i> in the first isolation. In an in vitro assay, our isolate grew well at 28 °C, but no propagation was observed at 37 °C. Furthermore, the isolate showed strong CPE on a mosquito-derived cultured cell line (C6/36), and its propagation slightly increased when co-cultured with C6/36 cells. To our knowledge, this is the third report of the isolation of <i>S. cantharicola</i> from mosquitoes and the first case in Asia. Our findings provide epidemiological data on <i>S. cantharicola</i> distribution in the region. |
| first_indexed | 2024-03-10T03:52:24Z |
| format | Article |
| id | doaj.art-12a8e16af1b146e7aad56ac9c286fad2 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T03:52:24Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj.art-12a8e16af1b146e7aad56ac9c286fad22023-11-23T08:52:16ZengMDPI AGInsects2075-44502021-11-011212105610.3390/insects12121056Isolation and Characterization of Mosquito-Associated <i>Spiroplasma cantharicola</i> from <i>Aedes japonicus</i> Collected in Hokkaido, JapanMakoto Shimooka0Yoshimi Sakurai1Yasukazu Muramatsu2Leo Uchida3School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Hokkaido, JapanSchool of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Hokkaido, JapanSchool of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Hokkaido, JapanSchool of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Hokkaido, JapanSpecies of the genus <i>Spiroplasma</i> are common within arthropods and plants worldwide. Mosquito-associated <i>Spiroplasma</i> spp. have been reported to show pathogenicity toward mosquitoes, which serve as vectors of several infectious diseases that have detrimental effects on public health. Although <i>Spiroplasma</i> spp. are expected to have potential use as biological vector-control tools, characteristics such as their distribution, host species, and cytopathogenic effects (CPEs) are not well understood. In this study, we isolated a <i>Spiroplasma</i> sp. from a female <i>Aedes japonicus</i> collected in Hokkaido, northern Japan. Phylogenetic analysis based on the 16S rRNA gene sequence indicated our isolate was closely related to <i>S. cantharicola</i>. We screened 103 mosquito pools consisting of 3 genera and 9 species, but only detected <i>S. cantharicola</i> in the first isolation. In an in vitro assay, our isolate grew well at 28 °C, but no propagation was observed at 37 °C. Furthermore, the isolate showed strong CPE on a mosquito-derived cultured cell line (C6/36), and its propagation slightly increased when co-cultured with C6/36 cells. To our knowledge, this is the third report of the isolation of <i>S. cantharicola</i> from mosquitoes and the first case in Asia. Our findings provide epidemiological data on <i>S. cantharicola</i> distribution in the region.https://www.mdpi.com/2075-4450/12/12/1056<i>Aedes japonicus</i>C6/36 cellscytopathogenic effectJapanmosquito<i>Spiroplasma cantharicola</i> |
| spellingShingle | Makoto Shimooka Yoshimi Sakurai Yasukazu Muramatsu Leo Uchida Isolation and Characterization of Mosquito-Associated <i>Spiroplasma cantharicola</i> from <i>Aedes japonicus</i> Collected in Hokkaido, Japan Insects <i>Aedes japonicus</i> C6/36 cells cytopathogenic effect Japan mosquito <i>Spiroplasma cantharicola</i> |
| title | Isolation and Characterization of Mosquito-Associated <i>Spiroplasma cantharicola</i> from <i>Aedes japonicus</i> Collected in Hokkaido, Japan |
| title_full | Isolation and Characterization of Mosquito-Associated <i>Spiroplasma cantharicola</i> from <i>Aedes japonicus</i> Collected in Hokkaido, Japan |
| title_fullStr | Isolation and Characterization of Mosquito-Associated <i>Spiroplasma cantharicola</i> from <i>Aedes japonicus</i> Collected in Hokkaido, Japan |
| title_full_unstemmed | Isolation and Characterization of Mosquito-Associated <i>Spiroplasma cantharicola</i> from <i>Aedes japonicus</i> Collected in Hokkaido, Japan |
| title_short | Isolation and Characterization of Mosquito-Associated <i>Spiroplasma cantharicola</i> from <i>Aedes japonicus</i> Collected in Hokkaido, Japan |
| title_sort | isolation and characterization of mosquito associated i spiroplasma cantharicola i from i aedes japonicus i collected in hokkaido japan |
| topic | <i>Aedes japonicus</i> C6/36 cells cytopathogenic effect Japan mosquito <i>Spiroplasma cantharicola</i> |
| url | https://www.mdpi.com/2075-4450/12/12/1056 |
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