Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance
Abstract Background In Colombia, Rhodnius prolixus and Triatoma dimidiata are the main domestic triatomine species known to transmit T. cruzi. However, there are multiple reports of T. cruzi transmission involving secondary vectors. In this work, we carried out an eco-epidemiological study on Margar...
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| Format: | Article |
| Language: | English |
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BMC
2015-09-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-015-1100-2 |
| _version_ | 1827933744554573824 |
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| author | Omar Cantillo-Barraza Edilson Garcés Andrés Gómez-Palacio Luis A. Cortés André Pereira Paula L. Marcet Ana M. Jansen Omar Triana-Chávez |
| author_facet | Omar Cantillo-Barraza Edilson Garcés Andrés Gómez-Palacio Luis A. Cortés André Pereira Paula L. Marcet Ana M. Jansen Omar Triana-Chávez |
| author_sort | Omar Cantillo-Barraza |
| collection | DOAJ |
| description | Abstract Background In Colombia, Rhodnius prolixus and Triatoma dimidiata are the main domestic triatomine species known to transmit T. cruzi. However, there are multiple reports of T. cruzi transmission involving secondary vectors. In this work, we carried out an eco-epidemiological study on Margarita Island, located in the Caribbean region of Colombia, where Chagas disease is associated with non-domiciliated vectors. Methods To understand the transmission dynamics of Trypanosoma cruzi in this area, we designed a comprehensive, multi-faceted study including the following: (i) entomological evaluation through a community-based insect-surveillance campaign, blood meal source determination and T. cruzi infection rate estimation in triatomine insects; (ii) serological determination of T. cruzi prevalence in children under 15 years old, as well as in domestic dogs and synanthropic mammals; (iii) evaluation of T. cruzi transmission capacity in dogs and Didelphis marsupialis, and (iv) genetic characterization of T. cruzi isolates targeting spliced-leader intergene region (SL-IR) genotypes. Results Out of the 124 triatomines collected, 94 % were Triatoma maculata, and 71.6 % of them were infected with T. cruzi. Blood-meal source analysis showed that T. maculata feeds on multiple hosts, including humans and domestic dogs. Serological analysis indicated 2 of 803 children were infected, representing a prevalence of 0.25 %. The prevalence in domestic dogs was 71.6 % (171/224). Domestic dogs might not be competent reservoir hosts, as inferred from negative T. cruzi xenodiagnosis and haemoculture tests. However, 61.5 % (8/13) of D. marsupialis, the most abundant synanthropic mammal captured, were T. cruzi-positive on xenodiagnosis and haemocultures. Conclusions This study reveals the role of peridomestic T. maculata and dogs in T. cruzi persistence in this region and presents evidence that D. marsupialis are a reservoir mediating peridomestic-zoonotic cycles. This picture reflects the complexity of the transmission dynamics of T. cruzi in an endemic area with non-domiciliated vectors where active human infection exists. There is an ongoing need to control peridomestic T. maculata populations and to implement continuous reservoir surveillance strategies with community participation. |
| first_indexed | 2024-03-13T07:29:05Z |
| format | Article |
| id | doaj.art-12a8e89c93854aec8b64821592f72d8d |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:05Z |
| publishDate | 2015-09-01 |
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| series | Parasites & Vectors |
| spelling | doaj.art-12a8e89c93854aec8b64821592f72d8d2023-06-04T11:10:06ZengBMCParasites & Vectors1756-33052015-09-018111010.1186/s13071-015-1100-2Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenanceOmar Cantillo-Barraza0Edilson Garcés1Andrés Gómez-Palacio2Luis A. Cortés3André Pereira4Paula L. Marcet5Ana M. Jansen6Omar Triana-Chávez7Grupo BCEI, Universidad de Antioquia UdeAGrupo BCEI, Universidad de Antioquia UdeAGrupo BCEI, Universidad de Antioquia UdeAUnidad de Entomología Médica, Secretaría de Salud del Departamento de BolívarLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZCenters for Disease Control and Prevention (CDC), Division of Parasitic Diseases and Malaria, Entomology BranchLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZGrupo BCEI, Universidad de Antioquia UdeAAbstract Background In Colombia, Rhodnius prolixus and Triatoma dimidiata are the main domestic triatomine species known to transmit T. cruzi. However, there are multiple reports of T. cruzi transmission involving secondary vectors. In this work, we carried out an eco-epidemiological study on Margarita Island, located in the Caribbean region of Colombia, where Chagas disease is associated with non-domiciliated vectors. Methods To understand the transmission dynamics of Trypanosoma cruzi in this area, we designed a comprehensive, multi-faceted study including the following: (i) entomological evaluation through a community-based insect-surveillance campaign, blood meal source determination and T. cruzi infection rate estimation in triatomine insects; (ii) serological determination of T. cruzi prevalence in children under 15 years old, as well as in domestic dogs and synanthropic mammals; (iii) evaluation of T. cruzi transmission capacity in dogs and Didelphis marsupialis, and (iv) genetic characterization of T. cruzi isolates targeting spliced-leader intergene region (SL-IR) genotypes. Results Out of the 124 triatomines collected, 94 % were Triatoma maculata, and 71.6 % of them were infected with T. cruzi. Blood-meal source analysis showed that T. maculata feeds on multiple hosts, including humans and domestic dogs. Serological analysis indicated 2 of 803 children were infected, representing a prevalence of 0.25 %. The prevalence in domestic dogs was 71.6 % (171/224). Domestic dogs might not be competent reservoir hosts, as inferred from negative T. cruzi xenodiagnosis and haemoculture tests. However, 61.5 % (8/13) of D. marsupialis, the most abundant synanthropic mammal captured, were T. cruzi-positive on xenodiagnosis and haemocultures. Conclusions This study reveals the role of peridomestic T. maculata and dogs in T. cruzi persistence in this region and presents evidence that D. marsupialis are a reservoir mediating peridomestic-zoonotic cycles. This picture reflects the complexity of the transmission dynamics of T. cruzi in an endemic area with non-domiciliated vectors where active human infection exists. There is an ongoing need to control peridomestic T. maculata populations and to implement continuous reservoir surveillance strategies with community participation.https://doi.org/10.1186/s13071-015-1100-2ColombiaRisk factors for Chagas diseaseTrypanosoma cruziSeroprevalenceDogsNon-domiciliated vectors |
| spellingShingle | Omar Cantillo-Barraza Edilson Garcés Andrés Gómez-Palacio Luis A. Cortés André Pereira Paula L. Marcet Ana M. Jansen Omar Triana-Chávez Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance Parasites & Vectors Colombia Risk factors for Chagas disease Trypanosoma cruzi Seroprevalence Dogs Non-domiciliated vectors |
| title | Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance |
| title_full | Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance |
| title_fullStr | Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance |
| title_full_unstemmed | Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance |
| title_short | Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance |
| title_sort | eco epidemiological study of an endemic chagas disease region in northern colombia reveals the importance of triatoma maculata hemiptera reduviidae dogs and didelphis marsupialis in trypanosoma cruzi maintenance |
| topic | Colombia Risk factors for Chagas disease Trypanosoma cruzi Seroprevalence Dogs Non-domiciliated vectors |
| url | https://doi.org/10.1186/s13071-015-1100-2 |
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