Replenishing NAD+ content reduces aspects of striated muscle disease in a dog model of Duchenne muscular dystrophy
Abstract Duchenne muscular dystrophy (DMD) is an X-linked disease caused by mutations in DMD gene and loss of the protein dystrophin, which ultimately leads to myofiber membrane fragility and necrosis, with eventual muscle atrophy and contractures. Affected boys typically die in their second or thir...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Skeletal Muscle |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13395-023-00328-w |
| _version_ | 1797397657448087552 |
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| author | Déborah Cardoso Inès Barthélémy Stéphane Blot Antoine Muchir |
| author_facet | Déborah Cardoso Inès Barthélémy Stéphane Blot Antoine Muchir |
| author_sort | Déborah Cardoso |
| collection | DOAJ |
| description | Abstract Duchenne muscular dystrophy (DMD) is an X-linked disease caused by mutations in DMD gene and loss of the protein dystrophin, which ultimately leads to myofiber membrane fragility and necrosis, with eventual muscle atrophy and contractures. Affected boys typically die in their second or third decade due to either respiratory failure or cardiomyopathy. Among the developed therapeutic strategies for DMD, gene therapy approaches partially restore micro-dystrophin or quasi-dystrophin expression. However, despite extensive attempts to develop definitive therapies for DMD, the standard of care remains corticosteroid, which has only palliative benefits. Animal models have played a key role in studies of DMD pathogenesis and treatment development. The golden retriever muscular dystrophy (GRMD) dog displays a phenotype aligning with the progressive course of DMD. Therefore, canine studies may translate better to humans. Recent studies suggested that nicotinamide adenine dinucleotide (NAD+) cellular content could be a critical determinant for striated muscle function. We showed here that NAD+ content was decreased in the striated muscles of GRMD, leading to an alteration of one of NAD+ co-substrate enzymes, PARP-1. Moreover, we showed that boosting NAD+ content using nicotinamide (NAM), a natural NAD+ precursor, modestly reduces aspects of striated muscle disease. Collectively, our results provide mechanistic insights into DMD. |
| first_indexed | 2024-03-09T01:13:21Z |
| format | Article |
| id | doaj.art-12a9f740a33e40718a3a37a61784efa2 |
| institution | Directory Open Access Journal |
| issn | 2044-5040 |
| language | English |
| last_indexed | 2024-03-09T01:13:21Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Skeletal Muscle |
| spelling | doaj.art-12a9f740a33e40718a3a37a61784efa22023-12-10T12:36:17ZengBMCSkeletal Muscle2044-50402023-12-0113111110.1186/s13395-023-00328-wReplenishing NAD+ content reduces aspects of striated muscle disease in a dog model of Duchenne muscular dystrophyDéborah Cardoso0Inès Barthélémy1Stéphane Blot2Antoine Muchir3Center of Research in Myology, Institute of Myology, INSERM, Sorbonne University“Biology of the Neuromuscular System” Team, U955 IMRB, INSERM, Univ Paris-Est Créteil“Biology of the Neuromuscular System” Team, U955 IMRB, INSERM, Univ Paris-Est CréteilCenter of Research in Myology, Institute of Myology, INSERM, Sorbonne UniversityAbstract Duchenne muscular dystrophy (DMD) is an X-linked disease caused by mutations in DMD gene and loss of the protein dystrophin, which ultimately leads to myofiber membrane fragility and necrosis, with eventual muscle atrophy and contractures. Affected boys typically die in their second or third decade due to either respiratory failure or cardiomyopathy. Among the developed therapeutic strategies for DMD, gene therapy approaches partially restore micro-dystrophin or quasi-dystrophin expression. However, despite extensive attempts to develop definitive therapies for DMD, the standard of care remains corticosteroid, which has only palliative benefits. Animal models have played a key role in studies of DMD pathogenesis and treatment development. The golden retriever muscular dystrophy (GRMD) dog displays a phenotype aligning with the progressive course of DMD. Therefore, canine studies may translate better to humans. Recent studies suggested that nicotinamide adenine dinucleotide (NAD+) cellular content could be a critical determinant for striated muscle function. We showed here that NAD+ content was decreased in the striated muscles of GRMD, leading to an alteration of one of NAD+ co-substrate enzymes, PARP-1. Moreover, we showed that boosting NAD+ content using nicotinamide (NAM), a natural NAD+ precursor, modestly reduces aspects of striated muscle disease. Collectively, our results provide mechanistic insights into DMD.https://doi.org/10.1186/s13395-023-00328-wDuchenne muscular dystrophyGRMDNAD+Nicotinamide |
| spellingShingle | Déborah Cardoso Inès Barthélémy Stéphane Blot Antoine Muchir Replenishing NAD+ content reduces aspects of striated muscle disease in a dog model of Duchenne muscular dystrophy Skeletal Muscle Duchenne muscular dystrophy GRMD NAD+ Nicotinamide |
| title | Replenishing NAD+ content reduces aspects of striated muscle disease in a dog model of Duchenne muscular dystrophy |
| title_full | Replenishing NAD+ content reduces aspects of striated muscle disease in a dog model of Duchenne muscular dystrophy |
| title_fullStr | Replenishing NAD+ content reduces aspects of striated muscle disease in a dog model of Duchenne muscular dystrophy |
| title_full_unstemmed | Replenishing NAD+ content reduces aspects of striated muscle disease in a dog model of Duchenne muscular dystrophy |
| title_short | Replenishing NAD+ content reduces aspects of striated muscle disease in a dog model of Duchenne muscular dystrophy |
| title_sort | replenishing nad content reduces aspects of striated muscle disease in a dog model of duchenne muscular dystrophy |
| topic | Duchenne muscular dystrophy GRMD NAD+ Nicotinamide |
| url | https://doi.org/10.1186/s13395-023-00328-w |
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