Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia
Background: Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology. Objective: To compare the level of intracellular anion superoxide (O2–), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and no...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Shahid Sadoughi University of Medical Sciences
2017-09-01
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| Series: | International Journal of Reproductive BioMedicine |
| Online Access: | http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/2567/1137 |
| _version_ | 1818108980071759872 |
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| author | Parvin Sabeti Fardin Amidi Seyed Mahdi Kalantar Mohammad Ali Sedighi Gilani Soheila Pourmasumi Atefeh Najafi Ali Reza Talebi |
| author_facet | Parvin Sabeti Fardin Amidi Seyed Mahdi Kalantar Mohammad Ali Sedighi Gilani Soheila Pourmasumi Atefeh Najafi Ali Reza Talebi |
| author_sort | Parvin Sabeti |
| collection | DOAJ |
| description | Background: Teratoasthenozoospermia (TA) is a severe form of male infertility
with no clear etiology.
Objective: To compare the level of intracellular anion superoxide (O2–), heat shock
protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between
teratoasthenozoospermic and normozoospermic men.
Materials and Methods: In this case- control study, semen samples of 20 infertile
men, with TA (with normal morphology lower than 4%_ and total motility lower
than 40% ) as the case group and 20 normozoospermic fertile men as the control
group were evaluated for intracellular O2
– and HSPA2 by flow cytometry and
protamine deficiency by Chromomycin A3 (CMA3) test.
Results: The rate of CMA3+ spermatozoa in the case group was higher than
controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were
significantly lower than controls (p=0.001). Also, intracellular O2
– levels in the case
group were significantly higher than controls (p=0.001) and had positive
correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm
(r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81,
p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between
intracellular O2
– and HSPA2 in the case group (r=0.041, p=0.79).
Conclusion: We suggest that the increase in intracellular O2
–, decrease in
spermatozoa HSPA2+, and high percentages of spermatozoa with immature
chromatin might be considered as etiologies of infertility in TA patients |
| first_indexed | 2024-12-11T02:23:58Z |
| format | Article |
| id | doaj.art-12ae3be543834f2eaf59ffed25bf6b0b |
| institution | Directory Open Access Journal |
| issn | 2476-4108 2476-3772 |
| language | English |
| last_indexed | 2024-12-11T02:23:58Z |
| publishDate | 2017-09-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-12ae3be543834f2eaf59ffed25bf6b0b2022-12-22T01:23:58ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722017-09-01155279286Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermiaParvin Sabeti0Fardin Amidi1Seyed Mahdi Kalantar2Mohammad Ali Sedighi Gilani3Soheila Pourmasumi4Atefeh Najafi5Ali Reza Talebi6Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IranDepartment Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Urology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, IranResearch and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranBackground: Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology. Objective: To compare the level of intracellular anion superoxide (O2–), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men. Materials and Methods: In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4%_ and total motility lower than 40% ) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O2 – and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test. Results: The rate of CMA3+ spermatozoa in the case group was higher than controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were significantly lower than controls (p=0.001). Also, intracellular O2 – levels in the case group were significantly higher than controls (p=0.001) and had positive correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm (r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81, p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between intracellular O2 – and HSPA2 in the case group (r=0.041, p=0.79). Conclusion: We suggest that the increase in intracellular O2 –, decrease in spermatozoa HSPA2+, and high percentages of spermatozoa with immature chromatin might be considered as etiologies of infertility in TA patientshttp://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/2567/1137 |
| spellingShingle | Parvin Sabeti Fardin Amidi Seyed Mahdi Kalantar Mohammad Ali Sedighi Gilani Soheila Pourmasumi Atefeh Najafi Ali Reza Talebi Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia International Journal of Reproductive BioMedicine |
| title | Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia |
| title_full | Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia |
| title_fullStr | Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia |
| title_full_unstemmed | Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia |
| title_short | Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia |
| title_sort | evaluation of intracellular anion superoxide level heat shock protein a2 and protamine positive spermatozoa percentages in teratoasthenozoospermia |
| url | http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/2567/1137 |
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