Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes
Summary: Glia, and in particular astrocytes, are one of the major players in neurological and neuroinflammatory disorders. Here, we present a protocol to efficiently generate inflammatory responsive astrocytes from human induced pluripotent stem cells in a monolayer culture. We describe steps for ne...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001004 |
| _version_ | 1811158953775595520 |
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| author | Anna Maria Sole Giordano Monah Abou Alezz Ivan Merelli Anna Kajaste-Rudnitski |
| author_facet | Anna Maria Sole Giordano Monah Abou Alezz Ivan Merelli Anna Kajaste-Rudnitski |
| author_sort | Anna Maria Sole Giordano |
| collection | DOAJ |
| description | Summary: Glia, and in particular astrocytes, are one of the major players in neurological and neuroinflammatory disorders. Here, we present a protocol to efficiently generate inflammatory responsive astrocytes from human induced pluripotent stem cells in a monolayer culture. We describe steps for neural differentiation to reach a homogeneous population of neural progenitor cells, followed by their differentiation into neural/glial progenitors. Finally, we detail enrichment to a 90% pure inflammatory responsive astrocyte population.For complete details on the use and execution of this protocol, please refer to Giordano et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T05:33:03Z |
| format | Article |
| id | doaj.art-12b310b29ce34e2da8ae4f486fb6b1a2 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T05:33:03Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-12b310b29ce34e2da8ae4f486fb6b1a22023-03-07T04:06:31ZengElsevierSTAR Protocols2666-16672023-03-0141102142Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytesAnna Maria Sole Giordano0Monah Abou Alezz1Ivan Merelli2Anna Kajaste-Rudnitski3San Raffaele Telethon Institute for Gene Therapy, IRCCS San Raffaele Scientific Institute, Milan, Italy; Corresponding authorSan Raffaele Telethon Institute for Gene Therapy, IRCCS San Raffaele Scientific Institute, Milan, ItalySan Raffaele Telethon Institute for Gene Therapy, IRCCS San Raffaele Scientific Institute, Milan, ItalySan Raffaele Telethon Institute for Gene Therapy, IRCCS San Raffaele Scientific Institute, Milan, Italy; Corresponding authorSummary: Glia, and in particular astrocytes, are one of the major players in neurological and neuroinflammatory disorders. Here, we present a protocol to efficiently generate inflammatory responsive astrocytes from human induced pluripotent stem cells in a monolayer culture. We describe steps for neural differentiation to reach a homogeneous population of neural progenitor cells, followed by their differentiation into neural/glial progenitors. Finally, we detail enrichment to a 90% pure inflammatory responsive astrocyte population.For complete details on the use and execution of this protocol, please refer to Giordano et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723001004Cell BiologyCell DifferentiationImmunologyMolecular BiologyNeuroscienceStem Cells |
| spellingShingle | Anna Maria Sole Giordano Monah Abou Alezz Ivan Merelli Anna Kajaste-Rudnitski Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes STAR Protocols Cell Biology Cell Differentiation Immunology Molecular Biology Neuroscience Stem Cells |
| title | Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes |
| title_full | Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes |
| title_fullStr | Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes |
| title_full_unstemmed | Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes |
| title_short | Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes |
| title_sort | protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes |
| topic | Cell Biology Cell Differentiation Immunology Molecular Biology Neuroscience Stem Cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723001004 |
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