Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes

Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes

Summary: Glia, and in particular astrocytes, are one of the major players in neurological and neuroinflammatory disorders. Here, we present a protocol to efficiently generate inflammatory responsive astrocytes from human induced pluripotent stem cells in a monolayer culture. We describe steps for ne...

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Bibliographic Details
Main Authors: Anna Maria Sole Giordano, Monah Abou Alezz, Ivan Merelli, Anna Kajaste-Rudnitski
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Differentiation
Immunology
Molecular Biology
Neuroscience
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001004

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