Application of Green Technology to Extract Clean and Safe Bioactive Compounds from <i>Tetradesmus obliquus</i> Biomass Grown in Poultry Wastewater
Microalgae are capable of assimilating nutrients from wastewater (WW), producing clean water and biomass rich in bioactive compounds that need to be recovered from inside the microalgal cell. This work investigated subcritical water (SW) extraction to collect high-value compounds from the microalga...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/5/2397 |
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| author | Jelena Vladić Jelena Molnar Jazić Alice Ferreira Snežana Maletić Dragoljub Cvetković Jasmina Agbaba Senka Vidović Luisa Gouveia |
| author_facet | Jelena Vladić Jelena Molnar Jazić Alice Ferreira Snežana Maletić Dragoljub Cvetković Jasmina Agbaba Senka Vidović Luisa Gouveia |
| author_sort | Jelena Vladić |
| collection | DOAJ |
| description | Microalgae are capable of assimilating nutrients from wastewater (WW), producing clean water and biomass rich in bioactive compounds that need to be recovered from inside the microalgal cell. This work investigated subcritical water (SW) extraction to collect high-value compounds from the microalga <i>Tetradesmus obliquus</i> after treating poultry WW. The treatment efficiency was evaluated in terms of total Kjeldahl nitrogen (TKN), phosphate, chemical oxygen demand (COD) and metals. <i>T. obliquus</i> was able to remove 77% TKN, 50% phosphate, 84% COD, and metals (48–89%) within legislation values. SW extraction was performed at 170 °C and 30 bar for 10 min. SW allowed the extraction of total phenols (1.073 mg GAE/mL extract) and total flavonoids (0.111 mg CAT/mL extract) with high antioxidant activity (IC<sub>50</sub> value, 7.18 µg/mL). The microalga was shown to be a source of organic compounds of commercial value (e.g., squalene). Finally, the SW conditions allowed the removal of pathogens and metals in the extracts and residues to values in accordance with legislation, assuring their safety for feed or agriculture applications. |
| first_indexed | 2024-03-11T07:16:11Z |
| format | Article |
| id | doaj.art-12b3edbbcce14b019a0345d1ed4b0523 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T07:16:11Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-12b3edbbcce14b019a0345d1ed4b05232023-11-17T08:16:07ZengMDPI AGMolecules1420-30492023-03-01285239710.3390/molecules28052397Application of Green Technology to Extract Clean and Safe Bioactive Compounds from <i>Tetradesmus obliquus</i> Biomass Grown in Poultry WastewaterJelena Vladić0Jelena Molnar Jazić1Alice Ferreira2Snežana Maletić3Dragoljub Cvetković4Jasmina Agbaba5Senka Vidović6Luisa Gouveia7Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, 21000 Novi Sad, SerbiaDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, 21000 Novi Sad, SerbiaLaboratório Nacional de Energia e Geologia IP, UBB-Bioenergy and Biorefineries Unit, Estrada Paço Lumiar, Edificio G, 1649-038 Lisbon, PortugalDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, 21000 Novi Sad, SerbiaDepartment of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, 21000 Novi Sad, SerbiaDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, 21000 Novi Sad, SerbiaDepartment of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, 21000 Novi Sad, SerbiaLaboratório Nacional de Energia e Geologia IP, UBB-Bioenergy and Biorefineries Unit, Estrada Paço Lumiar, Edificio G, 1649-038 Lisbon, PortugalMicroalgae are capable of assimilating nutrients from wastewater (WW), producing clean water and biomass rich in bioactive compounds that need to be recovered from inside the microalgal cell. This work investigated subcritical water (SW) extraction to collect high-value compounds from the microalga <i>Tetradesmus obliquus</i> after treating poultry WW. The treatment efficiency was evaluated in terms of total Kjeldahl nitrogen (TKN), phosphate, chemical oxygen demand (COD) and metals. <i>T. obliquus</i> was able to remove 77% TKN, 50% phosphate, 84% COD, and metals (48–89%) within legislation values. SW extraction was performed at 170 °C and 30 bar for 10 min. SW allowed the extraction of total phenols (1.073 mg GAE/mL extract) and total flavonoids (0.111 mg CAT/mL extract) with high antioxidant activity (IC<sub>50</sub> value, 7.18 µg/mL). The microalga was shown to be a source of organic compounds of commercial value (e.g., squalene). Finally, the SW conditions allowed the removal of pathogens and metals in the extracts and residues to values in accordance with legislation, assuring their safety for feed or agriculture applications.https://www.mdpi.com/1420-3049/28/5/2397subcritical water extractionnutrient recyclingmicroalgaemetal removalantioxidantsorganic profile |
| spellingShingle | Jelena Vladić Jelena Molnar Jazić Alice Ferreira Snežana Maletić Dragoljub Cvetković Jasmina Agbaba Senka Vidović Luisa Gouveia Application of Green Technology to Extract Clean and Safe Bioactive Compounds from <i>Tetradesmus obliquus</i> Biomass Grown in Poultry Wastewater Molecules subcritical water extraction nutrient recycling microalgae metal removal antioxidants organic profile |
| title | Application of Green Technology to Extract Clean and Safe Bioactive Compounds from <i>Tetradesmus obliquus</i> Biomass Grown in Poultry Wastewater |
| title_full | Application of Green Technology to Extract Clean and Safe Bioactive Compounds from <i>Tetradesmus obliquus</i> Biomass Grown in Poultry Wastewater |
| title_fullStr | Application of Green Technology to Extract Clean and Safe Bioactive Compounds from <i>Tetradesmus obliquus</i> Biomass Grown in Poultry Wastewater |
| title_full_unstemmed | Application of Green Technology to Extract Clean and Safe Bioactive Compounds from <i>Tetradesmus obliquus</i> Biomass Grown in Poultry Wastewater |
| title_short | Application of Green Technology to Extract Clean and Safe Bioactive Compounds from <i>Tetradesmus obliquus</i> Biomass Grown in Poultry Wastewater |
| title_sort | application of green technology to extract clean and safe bioactive compounds from i tetradesmus obliquus i biomass grown in poultry wastewater |
| topic | subcritical water extraction nutrient recycling microalgae metal removal antioxidants organic profile |
| url | https://www.mdpi.com/1420-3049/28/5/2397 |
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