Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells
The role of myosin light chain kinase (MLCK) in inducing podosomes was examined by confocal and electron microscopy. Removal of myosin from the actin core of podosomes using blebbistatin, a myosin inhibitor, resulted in the formation of smaller podosomes. Downregulation of MLCK by the transfection o...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Journal of Pharmacological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861315000584 |
| _version_ | 1818575372433752064 |
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| author | Hideyuki Tanaka Hong-Hui Wang Sean E. Thatcher Haruo Hagiwara Hiromi Takano-Ohmuro Kazuhiro Kohama |
| author_facet | Hideyuki Tanaka Hong-Hui Wang Sean E. Thatcher Haruo Hagiwara Hiromi Takano-Ohmuro Kazuhiro Kohama |
| author_sort | Hideyuki Tanaka |
| collection | DOAJ |
| description | The role of myosin light chain kinase (MLCK) in inducing podosomes was examined by confocal and electron microscopy. Removal of myosin from the actin core of podosomes using blebbistatin, a myosin inhibitor, resulted in the formation of smaller podosomes. Downregulation of MLCK by the transfection of MLCK small interfering RNA (siRNA) led to the failure of podosome formation. However, ML-7, an inhibitor of the kinase activity of MLCK, failed to inhibit podosome formation. Based on our previous report (Thatcher et al. J.Pharm.Sci. 116 116–127, 2011), we outlined the important role of the actin-binding activity of MLCK in producing smaller podosomes. |
| first_indexed | 2024-12-15T00:39:24Z |
| format | Article |
| id | doaj.art-12b5190e7371490d9e8a48dae6166a97 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-15T00:39:24Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-12b5190e7371490d9e8a48dae6166a972022-12-21T22:41:42ZengElsevierJournal of Pharmacological Sciences1347-86132015-06-011282788210.1016/j.jphs.2015.03.002Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cellsHideyuki Tanaka0Hong-Hui Wang1Sean E. Thatcher2Haruo Hagiwara3Hiromi Takano-Ohmuro4Kazuhiro Kohama5Department of Anatomy, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, JapanDepartment of Cellular and Molecular Medicine, University of California San Diego, 9500 Gillman Drive 0651, La Jolla, CA 92093-0651, USADepartment of Nutritional Sciences, University of Kentucky, Charles T.Wethington Bldg, 900 South Limestone, Lexington, KY 40536-0200, USADepartment of Anatomy, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, JapanResearch Institute of Pharmaceutical Sciences, Musashino University, Nishitokyo, Tokyo 202-8585, JapanResearch Institute of Pharmaceutical Sciences, Musashino University, Nishitokyo, Tokyo 202-8585, JapanThe role of myosin light chain kinase (MLCK) in inducing podosomes was examined by confocal and electron microscopy. Removal of myosin from the actin core of podosomes using blebbistatin, a myosin inhibitor, resulted in the formation of smaller podosomes. Downregulation of MLCK by the transfection of MLCK small interfering RNA (siRNA) led to the failure of podosome formation. However, ML-7, an inhibitor of the kinase activity of MLCK, failed to inhibit podosome formation. Based on our previous report (Thatcher et al. J.Pharm.Sci. 116 116–127, 2011), we outlined the important role of the actin-binding activity of MLCK in producing smaller podosomes.http://www.sciencedirect.com/science/article/pii/S1347861315000584PodosomeImmunoelectron microscopyMyosin light chain kinase |
| spellingShingle | Hideyuki Tanaka Hong-Hui Wang Sean E. Thatcher Haruo Hagiwara Hiromi Takano-Ohmuro Kazuhiro Kohama Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells Journal of Pharmacological Sciences Podosome Immunoelectron microscopy Myosin light chain kinase |
| title | Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells |
| title_full | Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells |
| title_fullStr | Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells |
| title_full_unstemmed | Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells |
| title_short | Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells |
| title_sort | electron microscopic examination of podosomes induced by phorbol 12 13 dibutyrate on the surface of a7r5 cells |
| topic | Podosome Immunoelectron microscopy Myosin light chain kinase |
| url | http://www.sciencedirect.com/science/article/pii/S1347861315000584 |
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