Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells

Electron microscopic examination of podosomes induced by phorbol 12, 13 dibutyrate on the surface of A7r5 cells

The role of myosin light chain kinase (MLCK) in inducing podosomes was examined by confocal and electron microscopy. Removal of myosin from the actin core of podosomes using blebbistatin, a myosin inhibitor, resulted in the formation of smaller podosomes. Downregulation of MLCK by the transfection o...

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Bibliographic Details
Main Authors: Hideyuki Tanaka, Hong-Hui Wang, Sean E. Thatcher, Haruo Hagiwara, Hiromi Takano-Ohmuro, Kazuhiro Kohama
Format: Article
Language:English
Published: Elsevier 2015-06-01
Series:Journal of Pharmacological Sciences
Subjects:
Podosome
Immunoelectron microscopy
Myosin light chain kinase
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861315000584

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