Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat
Fetal bovine serum (FBS) substitution remains one of the challenges to the realization of cultured meat production in the marketplace. In this study, three methods were developed to extract a substitute for FBS using egg white extract (EWE): using 25 mM CaCl2/2.5 % ammonium sulfate/citric acid (A);...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524000890 |
| _version_ | 1797244820666712064 |
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| author | Da Young Lee Dahee Han Seung Yun Lee Seung Hyeon Yun Juhyun Lee Ermie Mariano, Jr. Yeongwoo Choi Jin Soo Kim Jinmo Park Sun Jin Hur |
| author_facet | Da Young Lee Dahee Han Seung Yun Lee Seung Hyeon Yun Juhyun Lee Ermie Mariano, Jr. Yeongwoo Choi Jin Soo Kim Jinmo Park Sun Jin Hur |
| author_sort | Da Young Lee |
| collection | DOAJ |
| description | Fetal bovine serum (FBS) substitution remains one of the challenges to the realization of cultured meat production in the marketplace. In this study, three methods were developed to extract a substitute for FBS using egg white extract (EWE): using 25 mM CaCl2/2.5 % ammonium sulfate/citric acid (A); ethyl alcohol (B); and 5 % ammonium sulfate/citric acid (C). B EWE can effectively replace up to 50 % of FBS in growth media (10 % of the total). Ovalbumin in the extracts can promote cell proliferation, and components along the 12 kDa protein band have the potential to inhibit cell proliferation. Chick primary muscle cells applied with B EWE, an edible material that improved the cost and time efficiency of cultured meat production, effectively proliferated/differentiated. Therefore, EWE extracted using ethyl alcohol may be used as an FBS substitute to reduce animal sacrifices and should be considered a viable alternative to FBS for cultured meat. |
| first_indexed | 2024-03-07T20:03:45Z |
| format | Article |
| id | doaj.art-12b78c5e79d04023a05927d4e9fed494 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-24T19:17:05Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-12b78c5e79d04023a05927d4e9fed4942024-03-26T04:27:34ZengElsevierFood Chemistry: X2590-15752024-03-0121101202Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meatDa Young Lee0Dahee Han1Seung Yun Lee2Seung Hyeon Yun3Juhyun Lee4Ermie Mariano, Jr.5Yeongwoo Choi6Jin Soo Kim7Jinmo Park8Sun Jin Hur9Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDivision of Animal Science, Division of Applied Life Science (BK21 Four), Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, KoreaDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea; Corresponding author at: Department of Animal Science and Technology, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, Gyeonggi 17456, Republic of Korea.Fetal bovine serum (FBS) substitution remains one of the challenges to the realization of cultured meat production in the marketplace. In this study, three methods were developed to extract a substitute for FBS using egg white extract (EWE): using 25 mM CaCl2/2.5 % ammonium sulfate/citric acid (A); ethyl alcohol (B); and 5 % ammonium sulfate/citric acid (C). B EWE can effectively replace up to 50 % of FBS in growth media (10 % of the total). Ovalbumin in the extracts can promote cell proliferation, and components along the 12 kDa protein band have the potential to inhibit cell proliferation. Chick primary muscle cells applied with B EWE, an edible material that improved the cost and time efficiency of cultured meat production, effectively proliferated/differentiated. Therefore, EWE extracted using ethyl alcohol may be used as an FBS substitute to reduce animal sacrifices and should be considered a viable alternative to FBS for cultured meat.http://www.sciencedirect.com/science/article/pii/S2590157524000890FBS substituteSerum-free mediaEgg extractsCultured meatCell proliferation |
| spellingShingle | Da Young Lee Dahee Han Seung Yun Lee Seung Hyeon Yun Juhyun Lee Ermie Mariano, Jr. Yeongwoo Choi Jin Soo Kim Jinmo Park Sun Jin Hur Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat Food Chemistry: X FBS substitute Serum-free media Egg extracts Cultured meat Cell proliferation |
| title | Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat |
| title_full | Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat |
| title_fullStr | Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat |
| title_full_unstemmed | Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat |
| title_short | Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat |
| title_sort | preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat |
| topic | FBS substitute Serum-free media Egg extracts Cultured meat Cell proliferation |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524000890 |
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