Development and butyrylcholinesterase/monoamine oxidase inhibition potential of PVA-Berberis lycium nanofibers
The aim of this study was to evaluate the potential inhibitory effect of montmorillonite (MMT)-reinforced, glutaraldehyde-crosslinked PVA (polyvinyl alcohol) nanofibers loaded with root extract of Berberis lycium on monoamine oxidase A and B (MAO A and B) and butyrylcholinesterase (BChE) by using sl...
| Hlavní autoři: | , , , |
|---|---|
| Médium: | Článek |
| Jazyk: | English |
| Vydáno: |
De Gruyter
2022-03-01
|
| Edice: | Green Processing and Synthesis |
| Témata: | |
| On-line přístup: | https://doi.org/10.1515/gps-2022-0017 |
| _version_ | 1828383335569686528 |
|---|---|
| author | Ibrahim Nihal Abdalla Kaleem Saba Khan Abida Kalsoom Murtaza Ghulam |
| author_facet | Ibrahim Nihal Abdalla Kaleem Saba Khan Abida Kalsoom Murtaza Ghulam |
| author_sort | Ibrahim Nihal Abdalla |
| collection | DOAJ |
| description | The aim of this study was to evaluate the potential inhibitory effect of montmorillonite (MMT)-reinforced, glutaraldehyde-crosslinked PVA (polyvinyl alcohol) nanofibers loaded with root extract of Berberis lycium on monoamine oxidase A and B (MAO A and B) and butyrylcholinesterase (BChE) by using slightly modified Ellman’s test and Amplex Red monoamine oxidase assay, respectively. Enzyme inhibition studies of extract-loaded nanofibers showed significant inhibitory potential against MAO A, B, and BChE. There was an increase in enzyme inhibition with an increased extract concentration loaded to nanofibers. The fibers were characterized by TGA (thermal gravimetric analysis), SEM (scanning electron microscopy), XRD (X-ray diffractometry), and FTIR (Fourier-transform infra-red) spectroscopy to investigate thermal stability, morphology, structural changes, and functional groups in the nanofibers, respectively. SEM results of fabricated nanofibers reflected the beadless and smooth morphology of nanofibers with the porous structure. The contact angle measurements of fabricated nanofibers showed suitable hydrophilicity of nanofibers. The nanofibers loaded with the root extract of Berberis lycium have been found to be potent inhibitors of MAO A, B, and the BChE enzyme. |
| first_indexed | 2024-12-10T04:47:32Z |
| format | Article |
| id | doaj.art-12b87f3ca29e43f6b9c8336d6e76e10f |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-12-10T04:47:32Z |
| publishDate | 2022-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-12b87f3ca29e43f6b9c8336d6e76e10f2022-12-22T02:01:42ZengDe GruyterGreen Processing and Synthesis2191-95502022-03-0111122923710.1515/gps-2022-0017Development and butyrylcholinesterase/monoamine oxidase inhibition potential of PVA-Berberis lycium nanofibersIbrahim Nihal Abdalla0Kaleem Saba1Khan Abida Kalsoom2Murtaza Ghulam3Department of Clinical Sciences, College of Pharmacy and Health Sciences, Ajman University, Ajman, United Arab EmiratesDepartment of Chemistry, COMSATS University Islamabad, Lahore Campus, Abbottabad, PakistanDepartment of Chemistry, COMSATS University Islamabad, Lahore Campus, Abbottabad, PakistanDepartment of Pharmacy, COMSATS University Islamabad, Lahore Campus, Lahore, PakistanThe aim of this study was to evaluate the potential inhibitory effect of montmorillonite (MMT)-reinforced, glutaraldehyde-crosslinked PVA (polyvinyl alcohol) nanofibers loaded with root extract of Berberis lycium on monoamine oxidase A and B (MAO A and B) and butyrylcholinesterase (BChE) by using slightly modified Ellman’s test and Amplex Red monoamine oxidase assay, respectively. Enzyme inhibition studies of extract-loaded nanofibers showed significant inhibitory potential against MAO A, B, and BChE. There was an increase in enzyme inhibition with an increased extract concentration loaded to nanofibers. The fibers were characterized by TGA (thermal gravimetric analysis), SEM (scanning electron microscopy), XRD (X-ray diffractometry), and FTIR (Fourier-transform infra-red) spectroscopy to investigate thermal stability, morphology, structural changes, and functional groups in the nanofibers, respectively. SEM results of fabricated nanofibers reflected the beadless and smooth morphology of nanofibers with the porous structure. The contact angle measurements of fabricated nanofibers showed suitable hydrophilicity of nanofibers. The nanofibers loaded with the root extract of Berberis lycium have been found to be potent inhibitors of MAO A, B, and the BChE enzyme.https://doi.org/10.1515/gps-2022-0017poly(vinyl alcohol)montmorillonitebutyrylcholinesterasemonoamine oxidase a and benzyme inhibition |
| spellingShingle | Ibrahim Nihal Abdalla Kaleem Saba Khan Abida Kalsoom Murtaza Ghulam Development and butyrylcholinesterase/monoamine oxidase inhibition potential of PVA-Berberis lycium nanofibers Green Processing and Synthesis poly(vinyl alcohol) montmorillonite butyrylcholinesterase monoamine oxidase a and b enzyme inhibition |
| title | Development and butyrylcholinesterase/monoamine oxidase inhibition potential of PVA-Berberis lycium nanofibers |
| title_full | Development and butyrylcholinesterase/monoamine oxidase inhibition potential of PVA-Berberis lycium nanofibers |
| title_fullStr | Development and butyrylcholinesterase/monoamine oxidase inhibition potential of PVA-Berberis lycium nanofibers |
| title_full_unstemmed | Development and butyrylcholinesterase/monoamine oxidase inhibition potential of PVA-Berberis lycium nanofibers |
| title_short | Development and butyrylcholinesterase/monoamine oxidase inhibition potential of PVA-Berberis lycium nanofibers |
| title_sort | development and butyrylcholinesterase monoamine oxidase inhibition potential of pva berberis lycium nanofibers |
| topic | poly(vinyl alcohol) montmorillonite butyrylcholinesterase monoamine oxidase a and b enzyme inhibition |
| url | https://doi.org/10.1515/gps-2022-0017 |
| work_keys_str_mv | AT ibrahimnihalabdalla developmentandbutyrylcholinesterasemonoamineoxidaseinhibitionpotentialofpvaberberislyciumnanofibers AT kaleemsaba developmentandbutyrylcholinesterasemonoamineoxidaseinhibitionpotentialofpvaberberislyciumnanofibers AT khanabidakalsoom developmentandbutyrylcholinesterasemonoamineoxidaseinhibitionpotentialofpvaberberislyciumnanofibers AT murtazaghulam developmentandbutyrylcholinesterasemonoamineoxidaseinhibitionpotentialofpvaberberislyciumnanofibers |