Preparation and Optimization of Labeled Chitosan Nanoparticles and Evaluation of their Release from Transdermal Drug Delivery System
Biocompatible nanoparticles are widely used in biomedical engineering. In this study, chitosan nanoparticles were prepared using ionic gelation method in view of two determining factors namely method of adding chitosan into the tripolyphosphate (TPP) solution and thermal shock application. With rega...
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| Format: | Article |
| Language: | fas |
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Iran Polymer and Petrochemical Institute
2015-10-01
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| Series: | علوم و تکنولوژی پلیمر |
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| Online Access: | http://jips.ippi.ac.ir/article_1289_ad7d6c7b480d9c751b95ce3ac4689b81.pdf |
| _version_ | 1818885347207020544 |
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| author | Mohsen Sadeghi Fariba Ganji S. Mojtaba Taghizadeh |
| author_facet | Mohsen Sadeghi Fariba Ganji S. Mojtaba Taghizadeh |
| author_sort | Mohsen Sadeghi |
| collection | DOAJ |
| description | Biocompatible nanoparticles are widely used in biomedical engineering. In this study, chitosan nanoparticles were prepared using ionic gelation method in view of two determining factors namely method of adding chitosan into the tripolyphosphate (TPP) solution and thermal shock application. With regard to the concentration of chitosan and TPP solutions as two variables, the mean particle size of chitosan nanoparticles and their preparation yield were optimized using response surface method. According to previous studies and some preliminary experiments, the chitosan and TPP solution concentration ranges were determined to be 0.5-2.5 mg/mL and 0.25-1.25 mg/mL, respectively. The optimum values of 1.25 mg/mL and 0.6 mg/mL were obtained for chitosan and TPP solution concentrations in the order given. The optimized response value for the chitosan nanoparticles size was found to be 54 nm and preparation yield was 62%. The Zeta potential of resulting spherical nanoparticles was around 31 mV. Chitosan-fluorescein isothiocyanate (FITC) polymer was prepared based on the reaction between isothiocyanate functional group of FITC and primary amine functional group of chitosan. FTIR analysis was performed to demonstrate the presence of new bond formation. Labeled chitosan nanoparticles were prepared in the optimized condition using chitosan-FITC polymer. The release behavior of the labeled chitosan nanoparticles from transdermal patches was evaluated. The mean size of chitosan-FITC nanoparticles was determined to be 70 nm. Finally, it was shown that the chitosan nanoparticles were not able to release from acrylic adhesive film without using a method to speed up their diffusion. |
| first_indexed | 2024-12-19T16:04:00Z |
| format | Article |
| id | doaj.art-12b970ac10a546d0b08ef58ff22b5fe5 |
| institution | Directory Open Access Journal |
| issn | 1016-3255 2008-0883 |
| language | fas |
| last_indexed | 2024-12-19T16:04:00Z |
| publishDate | 2015-10-01 |
| publisher | Iran Polymer and Petrochemical Institute |
| record_format | Article |
| series | علوم و تکنولوژی پلیمر |
| spelling | doaj.art-12b970ac10a546d0b08ef58ff22b5fe52022-12-21T20:14:52ZfasIran Polymer and Petrochemical Instituteعلوم و تکنولوژی پلیمر1016-32552008-08832015-10-0128434433310.22063/jipst.2015.12891289Preparation and Optimization of Labeled Chitosan Nanoparticles and Evaluation of their Release from Transdermal Drug Delivery SystemMohsen Sadeghi0Fariba Ganji1S. Mojtaba Taghizadeh2Biomedical Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box: 14115-113, Tehran, IranBiomedical Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box: 14115-113, Tehran, IranNovel Drug Delivery Systems Department, Faculty of Polymer Science, Iran Polymer and Petrochemical Institute, P.O. Box: 14975-112, Tehran, IranBiocompatible nanoparticles are widely used in biomedical engineering. In this study, chitosan nanoparticles were prepared using ionic gelation method in view of two determining factors namely method of adding chitosan into the tripolyphosphate (TPP) solution and thermal shock application. With regard to the concentration of chitosan and TPP solutions as two variables, the mean particle size of chitosan nanoparticles and their preparation yield were optimized using response surface method. According to previous studies and some preliminary experiments, the chitosan and TPP solution concentration ranges were determined to be 0.5-2.5 mg/mL and 0.25-1.25 mg/mL, respectively. The optimum values of 1.25 mg/mL and 0.6 mg/mL were obtained for chitosan and TPP solution concentrations in the order given. The optimized response value for the chitosan nanoparticles size was found to be 54 nm and preparation yield was 62%. The Zeta potential of resulting spherical nanoparticles was around 31 mV. Chitosan-fluorescein isothiocyanate (FITC) polymer was prepared based on the reaction between isothiocyanate functional group of FITC and primary amine functional group of chitosan. FTIR analysis was performed to demonstrate the presence of new bond formation. Labeled chitosan nanoparticles were prepared in the optimized condition using chitosan-FITC polymer. The release behavior of the labeled chitosan nanoparticles from transdermal patches was evaluated. The mean size of chitosan-FITC nanoparticles was determined to be 70 nm. Finally, it was shown that the chitosan nanoparticles were not able to release from acrylic adhesive film without using a method to speed up their diffusion.http://jips.ippi.ac.ir/article_1289_ad7d6c7b480d9c751b95ce3ac4689b81.pdfchitosanionic gelationlabeled nanoparticlesoptimizationtransdermal drug delivery |
| spellingShingle | Mohsen Sadeghi Fariba Ganji S. Mojtaba Taghizadeh Preparation and Optimization of Labeled Chitosan Nanoparticles and Evaluation of their Release from Transdermal Drug Delivery System علوم و تکنولوژی پلیمر chitosan ionic gelation labeled nanoparticles optimization transdermal drug delivery |
| title | Preparation and Optimization of Labeled Chitosan Nanoparticles and Evaluation of their Release from Transdermal Drug Delivery System |
| title_full | Preparation and Optimization of Labeled Chitosan Nanoparticles and Evaluation of their Release from Transdermal Drug Delivery System |
| title_fullStr | Preparation and Optimization of Labeled Chitosan Nanoparticles and Evaluation of their Release from Transdermal Drug Delivery System |
| title_full_unstemmed | Preparation and Optimization of Labeled Chitosan Nanoparticles and Evaluation of their Release from Transdermal Drug Delivery System |
| title_short | Preparation and Optimization of Labeled Chitosan Nanoparticles and Evaluation of their Release from Transdermal Drug Delivery System |
| title_sort | preparation and optimization of labeled chitosan nanoparticles and evaluation of their release from transdermal drug delivery system |
| topic | chitosan ionic gelation labeled nanoparticles optimization transdermal drug delivery |
| url | http://jips.ippi.ac.ir/article_1289_ad7d6c7b480d9c751b95ce3ac4689b81.pdf |
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