Green Synthesis of Gold Nanoflowers Using <i>Rosmarinus officinalis</i> and <i>Helichrysum italicum</i> Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities
This study was concerned with the green synthesis of gold nanoflowers (AuNFs) using the bioactive constituents of <i>Rosmarinus officinalis</i> (rosemary) and <i>Helichrysum italicum</i> (immortelle) extracts, as reducer and stabilizer agents along with the determination of t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/11/1466 |
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| author | Nurhan Ertas Onmaz Dilek Demirezen Yilmaz Kálmán Imre Adriana Morar Candan Gungor Seyda Yilmaz Dursun Alp Gundog Adalet Dishan Viorel Herman Guven Gungor |
| author_facet | Nurhan Ertas Onmaz Dilek Demirezen Yilmaz Kálmán Imre Adriana Morar Candan Gungor Seyda Yilmaz Dursun Alp Gundog Adalet Dishan Viorel Herman Guven Gungor |
| author_sort | Nurhan Ertas Onmaz |
| collection | DOAJ |
| description | This study was concerned with the green synthesis of gold nanoflowers (AuNFs) using the bioactive constituents of <i>Rosmarinus officinalis</i> (rosemary) and <i>Helichrysum italicum</i> (immortelle) extracts, as reducer and stabilizer agents along with the determination of their antibacterial and antibiofilm activity against <i>E. coli</i>, <i>S. aureus</i>, and <i>S. epidermidis</i>. The AuNFs were characterized using STEM, UV–Vis, DLS, ZETA, FESEM-EDX, and FTIR techniques. The antibacterial and antibiofilm activity of the AuNFs were evaluated by microdilution broth and microtiter plate (MTP) tests, respectively. STEM and DLS analysis confirmed the flower-like morphology of gold nanoparticle clusters of <i>R. officinalis</i>-AuNFs (R-AuNFs) and <i>H. italicum</i>-AuNFs (H-AuNFs) with a size of 20–130 nm and 15–90 nm, respectively. The MICs of R-AuNFs were found to be 40 µg/mL for <i>E. coli</i> and <i>S. epidermidis</i> and 160 µg/mL for <i>S. aureus</i>. The MICs of H-AuNFs against all bacterial strains were 20 µg/mL. All tested AuNFs exhibited a strong dose-dependent antibiofilm activity against the test strains, and H-AuNFs was more effective than R-AuNFs. The green synthesis of AuNFs from the rosemary and immortelle extracts can be applied as a potential agent to overcome the growth of biofilm-producing microorganisms in food industries. |
| first_indexed | 2024-03-09T19:19:43Z |
| format | Article |
| id | doaj.art-12bc70b5683a4138856cd0e8845af758 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T19:19:43Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-12bc70b5683a4138856cd0e8845af7582023-11-24T03:26:49ZengMDPI AGAntibiotics2079-63822022-10-011111146610.3390/antibiotics11111466Green Synthesis of Gold Nanoflowers Using <i>Rosmarinus officinalis</i> and <i>Helichrysum italicum</i> Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm ActivitiesNurhan Ertas Onmaz0Dilek Demirezen Yilmaz1Kálmán Imre2Adriana Morar3Candan Gungor4Seyda Yilmaz5Dursun Alp Gundog6Adalet Dishan7Viorel Herman8Guven Gungor9Department of Veterinary Public Health, Faculty of Veterinary Medicine, Erciyes University, Kayseri 38039, TurkeyDepartment of Biology, Faculty of Science, Erciyes University, Kayseri 38039, TurkeyDepartment of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timișoara, 300645 Timisoara, RomaniaDepartment of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timișoara, 300645 Timisoara, RomaniaDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Erciyes University, Kayseri 38039, TurkeyNanoBiotech R&D Company, Erciyes Teknopark, Kayseri 38030, TurkeyDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Erciyes University, Kayseri 38039, TurkeyDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Erciyes University, Kayseri 38039, TurkeyDepartment of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timișoara, 300645 Timisoara, RomaniaDepartment of Biometrics, Faculty of Veterinary Medicine, Erciyes University, Kayseri 38039, TurkeyThis study was concerned with the green synthesis of gold nanoflowers (AuNFs) using the bioactive constituents of <i>Rosmarinus officinalis</i> (rosemary) and <i>Helichrysum italicum</i> (immortelle) extracts, as reducer and stabilizer agents along with the determination of their antibacterial and antibiofilm activity against <i>E. coli</i>, <i>S. aureus</i>, and <i>S. epidermidis</i>. The AuNFs were characterized using STEM, UV–Vis, DLS, ZETA, FESEM-EDX, and FTIR techniques. The antibacterial and antibiofilm activity of the AuNFs were evaluated by microdilution broth and microtiter plate (MTP) tests, respectively. STEM and DLS analysis confirmed the flower-like morphology of gold nanoparticle clusters of <i>R. officinalis</i>-AuNFs (R-AuNFs) and <i>H. italicum</i>-AuNFs (H-AuNFs) with a size of 20–130 nm and 15–90 nm, respectively. The MICs of R-AuNFs were found to be 40 µg/mL for <i>E. coli</i> and <i>S. epidermidis</i> and 160 µg/mL for <i>S. aureus</i>. The MICs of H-AuNFs against all bacterial strains were 20 µg/mL. All tested AuNFs exhibited a strong dose-dependent antibiofilm activity against the test strains, and H-AuNFs was more effective than R-AuNFs. The green synthesis of AuNFs from the rosemary and immortelle extracts can be applied as a potential agent to overcome the growth of biofilm-producing microorganisms in food industries.https://www.mdpi.com/2079-6382/11/11/1466antimicrobial activitybiofilms inhibition<i>Helichrysum italicum</i>gold nanoflower<i>Rosmarinus officinalis</i> |
| spellingShingle | Nurhan Ertas Onmaz Dilek Demirezen Yilmaz Kálmán Imre Adriana Morar Candan Gungor Seyda Yilmaz Dursun Alp Gundog Adalet Dishan Viorel Herman Guven Gungor Green Synthesis of Gold Nanoflowers Using <i>Rosmarinus officinalis</i> and <i>Helichrysum italicum</i> Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities Antibiotics antimicrobial activity biofilms inhibition <i>Helichrysum italicum</i> gold nanoflower <i>Rosmarinus officinalis</i> |
| title | Green Synthesis of Gold Nanoflowers Using <i>Rosmarinus officinalis</i> and <i>Helichrysum italicum</i> Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities |
| title_full | Green Synthesis of Gold Nanoflowers Using <i>Rosmarinus officinalis</i> and <i>Helichrysum italicum</i> Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities |
| title_fullStr | Green Synthesis of Gold Nanoflowers Using <i>Rosmarinus officinalis</i> and <i>Helichrysum italicum</i> Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities |
| title_full_unstemmed | Green Synthesis of Gold Nanoflowers Using <i>Rosmarinus officinalis</i> and <i>Helichrysum italicum</i> Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities |
| title_short | Green Synthesis of Gold Nanoflowers Using <i>Rosmarinus officinalis</i> and <i>Helichrysum italicum</i> Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities |
| title_sort | green synthesis of gold nanoflowers using i rosmarinus officinalis i and i helichrysum italicum i extracts comparative studies of their antimicrobial and antibiofilm activities |
| topic | antimicrobial activity biofilms inhibition <i>Helichrysum italicum</i> gold nanoflower <i>Rosmarinus officinalis</i> |
| url | https://www.mdpi.com/2079-6382/11/11/1466 |
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