Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development
Pectin modification and degradation are vital for plant development, although the underlying mechanisms are still not well understood. Furthermore, reports on the function of pectin in early pollen development are limited. We generated <i>OsPME</i>-FOX rice lines with little methyl-ester...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/8/1717 |
| _version_ | 1797603712022085632 |
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| author | Kazuya Hasegawa Ai Ichikawa Haruki Takeuchi Atsuko Nakamura Hiroaki Iwai |
| author_facet | Kazuya Hasegawa Ai Ichikawa Haruki Takeuchi Atsuko Nakamura Hiroaki Iwai |
| author_sort | Kazuya Hasegawa |
| collection | DOAJ |
| description | Pectin modification and degradation are vital for plant development, although the underlying mechanisms are still not well understood. Furthermore, reports on the function of pectin in early pollen development are limited. We generated <i>OsPME</i>-FOX rice lines with little methyl-esterified pectin even in the early-pollen mother-cell stage due to overexpression of the gene encoding pectin-methylesterase. Overexpression of <i>OsPME1</i> in rice increased the activity of PME, which decreased the degree of pectin methyl esterification in the cell wall. <i>OsPME1</i>-FOX grew normally and showed abnormal phenotypes in anther and pollen development, especially in terms of the pollen mother-cell stage. In addition, we examined modifications of cell-wall polysaccharides at the cellular level using antibodies against polysaccharides. Immunohistochemical staining using LM19 and LM20 showed that methyl-esterified pectin distribution and the pectin contents in pollen mother-cell wall decreased in <i>OsPME1</i>-FOX compared with the wild type. Thus, the maintenance of methyl-esterified pectin plays a role in degrading and maintaining the pollen mother-cell wall during microspore development. |
| first_indexed | 2024-03-11T04:35:38Z |
| format | Article |
| id | doaj.art-12bdda53546f444a9fb7b5293bac63e7 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:35:38Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-12bdda53546f444a9fb7b5293bac63e72023-11-17T21:00:38ZengMDPI AGPlants2223-77472023-04-01128171710.3390/plants12081717Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore DevelopmentKazuya Hasegawa0Ai Ichikawa1Haruki Takeuchi2Atsuko Nakamura3Hiroaki Iwai4Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, JapanInstitute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, JapanPectin modification and degradation are vital for plant development, although the underlying mechanisms are still not well understood. Furthermore, reports on the function of pectin in early pollen development are limited. We generated <i>OsPME</i>-FOX rice lines with little methyl-esterified pectin even in the early-pollen mother-cell stage due to overexpression of the gene encoding pectin-methylesterase. Overexpression of <i>OsPME1</i> in rice increased the activity of PME, which decreased the degree of pectin methyl esterification in the cell wall. <i>OsPME1</i>-FOX grew normally and showed abnormal phenotypes in anther and pollen development, especially in terms of the pollen mother-cell stage. In addition, we examined modifications of cell-wall polysaccharides at the cellular level using antibodies against polysaccharides. Immunohistochemical staining using LM19 and LM20 showed that methyl-esterified pectin distribution and the pectin contents in pollen mother-cell wall decreased in <i>OsPME1</i>-FOX compared with the wild type. Thus, the maintenance of methyl-esterified pectin plays a role in degrading and maintaining the pollen mother-cell wall during microspore development.https://www.mdpi.com/2223-7747/12/8/1717cell wallpectinpectin methylesterasepollen<i>Oryza sativa</i> |
| spellingShingle | Kazuya Hasegawa Ai Ichikawa Haruki Takeuchi Atsuko Nakamura Hiroaki Iwai Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development Plants cell wall pectin pectin methylesterase pollen <i>Oryza sativa</i> |
| title | Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development |
| title_full | Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development |
| title_fullStr | Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development |
| title_full_unstemmed | Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development |
| title_short | Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development |
| title_sort | maintenance of methyl esterified pectin level in pollen mother cell stages is required for microspore development |
| topic | cell wall pectin pectin methylesterase pollen <i>Oryza sativa</i> |
| url | https://www.mdpi.com/2223-7747/12/8/1717 |
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