Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring
Due to its anti-inflammatory and anti-oxidative effects, recent research has demonstrated that molecular hydrogen can serve as a new medical approach for depression, anxiety and traumatic brain injury. However, its potential effects on neurodevelopmental diseases, such as autism are still elusive. T...
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Frontiers Media S.A.
2018-08-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnbeh.2018.00170/full |
| _version_ | 1817992639615598592 |
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| author | Qingjun Guo Xi Yin Meng Qiao Yujiao Jia Dandan Chen Juan Shao Tyler W. Lebaron Yuan Gao Yuan Gao Haishui Shi Haishui Shi Haishui Shi Haishui Shi Bin Jia Bin Jia |
| author_facet | Qingjun Guo Xi Yin Meng Qiao Yujiao Jia Dandan Chen Juan Shao Tyler W. Lebaron Yuan Gao Yuan Gao Haishui Shi Haishui Shi Haishui Shi Haishui Shi Bin Jia Bin Jia |
| author_sort | Qingjun Guo |
| collection | DOAJ |
| description | Due to its anti-inflammatory and anti-oxidative effects, recent research has demonstrated that molecular hydrogen can serve as a new medical approach for depression, anxiety and traumatic brain injury. However, its potential effects on neurodevelopmental diseases, such as autism are still elusive. The present study aims to investigate the potential effects of hydrogen-rich water (HRW) administration on valproic acid (VPA)-induced autistic-like behavioral deficits, and the associated underlying mechanism in adolescent mice offspring. Pregnant ICR mice were randomly divided into five groups (n = 6). One group was injected with saline (NAV group) and provided hydrogen-free water. The other four groups were injected with VPA (600 mg/kg, intraperitoneally, i.p.) on pregnant day (PND) 12.5. One group was provided with hydrogen-free water (VEH group) and the other three groups were provided HRW at different segments, postnatal day 1 (PND 1) to PND 21 (PHV group), PND 13 to PND 21 (PVS group) or from PND 13 to postnatal day 42 (PVL group). Behavioral tests, including open field, novelty suppressed feeding (NSF), hot plate, social interaction (SI) and contextual fear memory tests were conducted between postnatal day 35–42. We found that HRW administration significantly reversed the autistic-like behaviors induced by maternal VPA exposure in the adolescent offspring of both male and female adolescent offspring. Furthermore, HRW administration significantly reversed the alternation of serum levels of interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α), but without any effects on the BDNF levels in maternal VPA-exposed mice offspring. These data suggest the need for additional research on HRW as a potential preventive strategy for autism and related disorders.Lay Summary: Maternal VPA injection induces autistic-like behavioral deficits in adolescent mice offspring. HRW administration ameliorates autistic-like behavioral deficits. HRW administration reverses the alternation of serum levels of IL-6 and TNF-α induced by VPA. |
| first_indexed | 2024-04-14T01:28:45Z |
| format | Article |
| id | doaj.art-12be88c564614848b9418954d163d497 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-14T01:28:45Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-12be88c564614848b9418954d163d4972022-12-22T02:20:16ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532018-08-011210.3389/fnbeh.2018.00170365854Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice OffspringQingjun Guo0Xi Yin1Meng Qiao2Yujiao Jia3Dandan Chen4Juan Shao5Tyler W. Lebaron6Yuan Gao7Yuan Gao8Haishui Shi9Haishui Shi10Haishui Shi11Haishui Shi12Bin Jia13Bin Jia14Department of Surgery, Hebei Medical University, Shijiazhuang, ChinaDepartment of Functional Region of Diagnosis, Hebei Medical University Fourth Hospital, Hebei Medical University, Shijiazhuang, ChinaCollege of Basic Medicine, Hebei Medical University, Shijiazhuang, ChinaCollege of Basic Medicine, Hebei Medical University, Shijiazhuang, ChinaCollege of Basic Medicine, Hebei Medical University, Shijiazhuang, ChinaDepartment of Senile Disease, The Third Hospital of Hebei Medical University, Hebei Medical University, Shijiazhuang, ChinaMolecular Hydrogen Foundation, Kissimmee, FL, United StatesDepartment of Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang, ChinaNeuroscience Research Center, Hebei Medical University, Shijiazhuang, ChinaDepartment of Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang, ChinaNeuroscience Research Center, Hebei Medical University, Shijiazhuang, ChinaHebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Shijiazhuang, ChinaCollaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Medicial University, Shijiazhuang, ChinaNeuroscience Research Center, Hebei Medical University, Shijiazhuang, China0Lingshui General Hospital, Lingshui, ChinaDue to its anti-inflammatory and anti-oxidative effects, recent research has demonstrated that molecular hydrogen can serve as a new medical approach for depression, anxiety and traumatic brain injury. However, its potential effects on neurodevelopmental diseases, such as autism are still elusive. The present study aims to investigate the potential effects of hydrogen-rich water (HRW) administration on valproic acid (VPA)-induced autistic-like behavioral deficits, and the associated underlying mechanism in adolescent mice offspring. Pregnant ICR mice were randomly divided into five groups (n = 6). One group was injected with saline (NAV group) and provided hydrogen-free water. The other four groups were injected with VPA (600 mg/kg, intraperitoneally, i.p.) on pregnant day (PND) 12.5. One group was provided with hydrogen-free water (VEH group) and the other three groups were provided HRW at different segments, postnatal day 1 (PND 1) to PND 21 (PHV group), PND 13 to PND 21 (PVS group) or from PND 13 to postnatal day 42 (PVL group). Behavioral tests, including open field, novelty suppressed feeding (NSF), hot plate, social interaction (SI) and contextual fear memory tests were conducted between postnatal day 35–42. We found that HRW administration significantly reversed the autistic-like behaviors induced by maternal VPA exposure in the adolescent offspring of both male and female adolescent offspring. Furthermore, HRW administration significantly reversed the alternation of serum levels of interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α), but without any effects on the BDNF levels in maternal VPA-exposed mice offspring. These data suggest the need for additional research on HRW as a potential preventive strategy for autism and related disorders.Lay Summary: Maternal VPA injection induces autistic-like behavioral deficits in adolescent mice offspring. HRW administration ameliorates autistic-like behavioral deficits. HRW administration reverses the alternation of serum levels of IL-6 and TNF-α induced by VPA.https://www.frontiersin.org/article/10.3389/fnbeh.2018.00170/fullmolecular hydrogenautismvalproic acidinflammationinterleukin 6tumor necrosis factor-α |
| spellingShingle | Qingjun Guo Xi Yin Meng Qiao Yujiao Jia Dandan Chen Juan Shao Tyler W. Lebaron Yuan Gao Yuan Gao Haishui Shi Haishui Shi Haishui Shi Haishui Shi Bin Jia Bin Jia Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring Frontiers in Behavioral Neuroscience molecular hydrogen autism valproic acid inflammation interleukin 6 tumor necrosis factor-α |
| title | Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring |
| title_full | Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring |
| title_fullStr | Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring |
| title_full_unstemmed | Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring |
| title_short | Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring |
| title_sort | hydrogen rich water ameliorates autistic like behavioral abnormalities in valproic acid treated adolescent mice offspring |
| topic | molecular hydrogen autism valproic acid inflammation interleukin 6 tumor necrosis factor-α |
| url | https://www.frontiersin.org/article/10.3389/fnbeh.2018.00170/full |
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