Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.
Understanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tro...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-05-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC4420505?pdf=render |
| _version_ | 1811269121345585152 |
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| author | Antonio V Bordería Ofer Isakov Gonzalo Moratorio Rasmus Henningsson Sonia Agüera-González Lindsey Organtini Nina F Gnädig Hervé Blanc Andrés Alcover Susan Hafenstein Magnus Fontes Noam Shomron Marco Vignuzzi |
| author_facet | Antonio V Bordería Ofer Isakov Gonzalo Moratorio Rasmus Henningsson Sonia Agüera-González Lindsey Organtini Nina F Gnädig Hervé Blanc Andrés Alcover Susan Hafenstein Magnus Fontes Noam Shomron Marco Vignuzzi |
| author_sort | Antonio V Bordería |
| collection | DOAJ |
| description | Understanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tropism and species jumps can occur. Here, we adapted Coxsackie virus B3 to a highly permissive and less permissive environment. Using deep sequencing and bioinformatics, we identified a multi-step adaptive process to adaptation involving residues in the receptor footprints that correlated with receptor availability and with increase in virus fitness in an environment-specific manner. We show that adaptation occurs by selection of a dominant mutation followed by group selection of minority variants that together, confer the fitness increase observed in the population, rather than selection of a single dominant genotype. |
| first_indexed | 2024-04-12T21:36:22Z |
| format | Article |
| id | doaj.art-12bf3c4d8949455489cae1b59f70dc80 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-12T21:36:22Z |
| publishDate | 2015-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-12bf3c4d8949455489cae1b59f70dc802022-12-22T03:15:53ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742015-05-01115e100483810.1371/journal.ppat.1004838Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.Antonio V BorderíaOfer IsakovGonzalo MoratorioRasmus HenningssonSonia Agüera-GonzálezLindsey OrgantiniNina F GnädigHervé BlancAndrés AlcoverSusan HafensteinMagnus FontesNoam ShomronMarco VignuzziUnderstanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tropism and species jumps can occur. Here, we adapted Coxsackie virus B3 to a highly permissive and less permissive environment. Using deep sequencing and bioinformatics, we identified a multi-step adaptive process to adaptation involving residues in the receptor footprints that correlated with receptor availability and with increase in virus fitness in an environment-specific manner. We show that adaptation occurs by selection of a dominant mutation followed by group selection of minority variants that together, confer the fitness increase observed in the population, rather than selection of a single dominant genotype.http://europepmc.org/articles/PMC4420505?pdf=render |
| spellingShingle | Antonio V Bordería Ofer Isakov Gonzalo Moratorio Rasmus Henningsson Sonia Agüera-González Lindsey Organtini Nina F Gnädig Hervé Blanc Andrés Alcover Susan Hafenstein Magnus Fontes Noam Shomron Marco Vignuzzi Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. PLoS Pathogens |
| title | Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. |
| title_full | Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. |
| title_fullStr | Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. |
| title_full_unstemmed | Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. |
| title_short | Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. |
| title_sort | group selection and contribution of minority variants during virus adaptation determines virus fitness and phenotype |
| url | http://europepmc.org/articles/PMC4420505?pdf=render |
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