Candida and Porphyromonas gingivalis: the effect on wound closure in vitro
Microorganisms play a role in oral mucositis after cancer therapy. The current study explored the hypothesis that Candida spp. alone and together with Porphyromonas gingivalis cause delayed healing of oral ulcerations due to the inhibition of wound closure. An in vitro scratch assay model was used t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-01-01
|
| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2017.1328266 |
| _version_ | 1818060351601639424 |
|---|---|
| author | Thijs M. Haverman Alexa M. G. A. Laheij Johannes J. de Soet Jan de Lange Frederik R. Rozema |
| author_facet | Thijs M. Haverman Alexa M. G. A. Laheij Johannes J. de Soet Jan de Lange Frederik R. Rozema |
| author_sort | Thijs M. Haverman |
| collection | DOAJ |
| description | Microorganisms play a role in oral mucositis after cancer therapy. The current study explored the hypothesis that Candida spp. alone and together with Porphyromonas gingivalis cause delayed healing of oral ulcerations due to the inhibition of wound closure. An in vitro scratch assay model was used to study the influence of viable and heat-killed Candida glabrata, Candida kefyr, and Candida albicans on cell migration of oral epithelial cells. Separately, the effect of conditioned medium of Candida spp. and the effect of a mixed infection of Candida spp. with P. gingivalis on wound closure was studied. In the presence of 10 viable C. glabrata or C. kefyr versus one epithelial cell, with a multiplicity of infection (MOI) of 10, the relative closure of the scratch was 26% and 17%, respectively. At a MOI of 1, this was 60% for C. glabrata and 78% for C. kefyr. The inhibition of oral epithelial cell migration challenged with either C. glabrata or C. kefyr together with P. gingivalis was stronger than the inhibition caused by one of both organisms separately. Candida spp. inhibit cell migration in vitro. A combination of Candida spp. and P. gingivalis inhibited cell migration more than either microorganism separately. |
| first_indexed | 2024-12-10T13:31:02Z |
| format | Article |
| id | doaj.art-12c07d2caa0d4b198ae7c5aafe3042f3 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-10T13:31:02Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-12c07d2caa0d4b198ae7c5aafe3042f32022-12-22T01:46:58ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972017-01-019110.1080/20002297.2017.13282661328266Candida and Porphyromonas gingivalis: the effect on wound closure in vitroThijs M. Haverman0Alexa M. G. A. Laheij1Johannes J. de Soet2Jan de Lange3Frederik R. Rozema4University of Amsterdam and the Vrije Universiteit AmsterdamUniversity of Amsterdam and the Vrije Universiteit AmsterdamUniversity of Amsterdam and the Vrije Universiteit AmsterdamAcademic Medical Center Amsterdam, University of AmsterdamUniversity of Amsterdam and the Vrije Universiteit AmsterdamMicroorganisms play a role in oral mucositis after cancer therapy. The current study explored the hypothesis that Candida spp. alone and together with Porphyromonas gingivalis cause delayed healing of oral ulcerations due to the inhibition of wound closure. An in vitro scratch assay model was used to study the influence of viable and heat-killed Candida glabrata, Candida kefyr, and Candida albicans on cell migration of oral epithelial cells. Separately, the effect of conditioned medium of Candida spp. and the effect of a mixed infection of Candida spp. with P. gingivalis on wound closure was studied. In the presence of 10 viable C. glabrata or C. kefyr versus one epithelial cell, with a multiplicity of infection (MOI) of 10, the relative closure of the scratch was 26% and 17%, respectively. At a MOI of 1, this was 60% for C. glabrata and 78% for C. kefyr. The inhibition of oral epithelial cell migration challenged with either C. glabrata or C. kefyr together with P. gingivalis was stronger than the inhibition caused by one of both organisms separately. Candida spp. inhibit cell migration in vitro. A combination of Candida spp. and P. gingivalis inhibited cell migration more than either microorganism separately.http://dx.doi.org/10.1080/20002297.2017.1328266In vitro scratch assayoral epithelial cellsre-epithelializationoral ulcerationCandida glabrataCandida kefyroral mucositiswound closurePorphyromonas gingivaliscell migration |
| spellingShingle | Thijs M. Haverman Alexa M. G. A. Laheij Johannes J. de Soet Jan de Lange Frederik R. Rozema Candida and Porphyromonas gingivalis: the effect on wound closure in vitro Journal of Oral Microbiology In vitro scratch assay oral epithelial cells re-epithelialization oral ulceration Candida glabrata Candida kefyr oral mucositis wound closure Porphyromonas gingivalis cell migration |
| title | Candida and Porphyromonas gingivalis: the effect on wound closure in vitro |
| title_full | Candida and Porphyromonas gingivalis: the effect on wound closure in vitro |
| title_fullStr | Candida and Porphyromonas gingivalis: the effect on wound closure in vitro |
| title_full_unstemmed | Candida and Porphyromonas gingivalis: the effect on wound closure in vitro |
| title_short | Candida and Porphyromonas gingivalis: the effect on wound closure in vitro |
| title_sort | candida and porphyromonas gingivalis the effect on wound closure in vitro |
| topic | In vitro scratch assay oral epithelial cells re-epithelialization oral ulceration Candida glabrata Candida kefyr oral mucositis wound closure Porphyromonas gingivalis cell migration |
| url | http://dx.doi.org/10.1080/20002297.2017.1328266 |
| work_keys_str_mv | AT thijsmhaverman candidaandporphyromonasgingivalistheeffectonwoundclosureinvitro AT alexamgalaheij candidaandporphyromonasgingivalistheeffectonwoundclosureinvitro AT johannesjdesoet candidaandporphyromonasgingivalistheeffectonwoundclosureinvitro AT jandelange candidaandporphyromonasgingivalistheeffectonwoundclosureinvitro AT frederikrrozema candidaandporphyromonasgingivalistheeffectonwoundclosureinvitro |