<i>Bacillus cereus</i> Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature
Cellular proteomes and exoproteomes are dynamic, allowing pathogens to respond to environmental conditions to sustain growth and virulence. <i>Bacillus cereus</i> is an important food-borne pathogen causing intoxication via emetic toxin and/or multiple protein exotoxins. Here, we compare...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/10/645 |
| _version_ | 1797551599510355968 |
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| author | Catherine Duport Ludivine Rousset Béatrice Alpha-Bazin Jean Armengaud |
| author_facet | Catherine Duport Ludivine Rousset Béatrice Alpha-Bazin Jean Armengaud |
| author_sort | Catherine Duport |
| collection | DOAJ |
| description | Cellular proteomes and exoproteomes are dynamic, allowing pathogens to respond to environmental conditions to sustain growth and virulence. <i>Bacillus cereus</i> is an important food-borne pathogen causing intoxication via emetic toxin and/or multiple protein exotoxins. Here, we compared the dynamics of the cellular proteome and exoproteome of emetic <i>B. cereus</i> cells grown at low (16 °C) and high (30 °C) temperature. Tandem mass spectrometry (MS/MS)-based shotgun proteomics analysis identified 2063 cellular proteins and 900 extracellular proteins. Hierarchical clustering following principal component analysis indicated that in <i>B. cereus</i> the abundance of a subset of these proteins—including cold-stress responders, and exotoxins non-hemolytic enterotoxin (NHE) and hemolysin I (cereolysin O (CLO))—decreased at low temperature, and that this subset governs the dynamics of the cellular proteome. NHE, and to a lesser extent CLO, also contributed significantly to exoproteome dynamics; with decreased abundances in the low-temperature exoproteome, especially in late growth stages. Our data therefore indicate that <i>B. cereus</i> may reduce its production of secreted protein toxins to maintain appropriate proteome dynamics, perhaps using catabolite repression to conserve energy for growth in cold-stress conditions, at the expense of virulence. |
| first_indexed | 2024-03-10T15:48:26Z |
| format | Article |
| id | doaj.art-12c0d381723441cc930e98b35366e675 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T15:48:26Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-12c0d381723441cc930e98b35366e6752023-11-20T16:14:24ZengMDPI AGToxins2072-66512020-10-01121064510.3390/toxins12100645<i>Bacillus cereus</i> Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low TemperatureCatherine Duport0Ludivine Rousset1Béatrice Alpha-Bazin2Jean Armengaud3Avignon University, Biology Department, INRAE, UMR SQPOV, F-84914 Avignon, FranceAvignon University, Biology Department, INRAE, UMR SQPOV, F-84914 Avignon, FranceDépartement Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI, 30200 Bagnols-sur-Cèze, FranceDépartement Médicaments et Technologies pour la Santé (DMTS), Université Paris Saclay, CEA, INRAE, SPI, 30200 Bagnols-sur-Cèze, FranceCellular proteomes and exoproteomes are dynamic, allowing pathogens to respond to environmental conditions to sustain growth and virulence. <i>Bacillus cereus</i> is an important food-borne pathogen causing intoxication via emetic toxin and/or multiple protein exotoxins. Here, we compared the dynamics of the cellular proteome and exoproteome of emetic <i>B. cereus</i> cells grown at low (16 °C) and high (30 °C) temperature. Tandem mass spectrometry (MS/MS)-based shotgun proteomics analysis identified 2063 cellular proteins and 900 extracellular proteins. Hierarchical clustering following principal component analysis indicated that in <i>B. cereus</i> the abundance of a subset of these proteins—including cold-stress responders, and exotoxins non-hemolytic enterotoxin (NHE) and hemolysin I (cereolysin O (CLO))—decreased at low temperature, and that this subset governs the dynamics of the cellular proteome. NHE, and to a lesser extent CLO, also contributed significantly to exoproteome dynamics; with decreased abundances in the low-temperature exoproteome, especially in late growth stages. Our data therefore indicate that <i>B. cereus</i> may reduce its production of secreted protein toxins to maintain appropriate proteome dynamics, perhaps using catabolite repression to conserve energy for growth in cold-stress conditions, at the expense of virulence.https://www.mdpi.com/2072-6651/12/10/645<i>Bacillus cereus</i>shotgun proteomicsexotoxinslow-temperature |
| spellingShingle | Catherine Duport Ludivine Rousset Béatrice Alpha-Bazin Jean Armengaud <i>Bacillus cereus</i> Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature Toxins <i>Bacillus cereus</i> shotgun proteomics exotoxins low-temperature |
| title | <i>Bacillus cereus</i> Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature |
| title_full | <i>Bacillus cereus</i> Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature |
| title_fullStr | <i>Bacillus cereus</i> Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature |
| title_full_unstemmed | <i>Bacillus cereus</i> Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature |
| title_short | <i>Bacillus cereus</i> Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature |
| title_sort | i bacillus cereus i decreases nhe and clo exotoxin synthesis to maintain appropriate proteome dynamics during growth at low temperature |
| topic | <i>Bacillus cereus</i> shotgun proteomics exotoxins low-temperature |
| url | https://www.mdpi.com/2072-6651/12/10/645 |
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