Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin Detection
Doxorubicin (DOX) is widely used in chemotherapy as an anti-tumor drug. However, DOX is highly cardio-, neuro- and cytotoxic. For this reason, the continuous monitoring of DOX concentrations in biofluids and tissues is important. Most methods for the determination of DOX concentrations are complex a...
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MDPI AG
2023-05-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/6/596 |
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| author | Svetlana A. Mescheryakova Ivan S. Matlakhov Pavel D. Strokin Daniil D. Drozd Irina Yu. Goryacheva Olga A. Goryacheva |
| author_facet | Svetlana A. Mescheryakova Ivan S. Matlakhov Pavel D. Strokin Daniil D. Drozd Irina Yu. Goryacheva Olga A. Goryacheva |
| author_sort | Svetlana A. Mescheryakova |
| collection | DOAJ |
| description | Doxorubicin (DOX) is widely used in chemotherapy as an anti-tumor drug. However, DOX is highly cardio-, neuro- and cytotoxic. For this reason, the continuous monitoring of DOX concentrations in biofluids and tissues is important. Most methods for the determination of DOX concentrations are complex and costly, and are designed to determine pure DOX. The purpose of this work is to demonstrate the capabilities of analytical nanosensors based on the quenching of the fluorescence of alloyed CdZnSeS/ZnS quantum dots (QDs) for operative DOX detection. To maximize the nanosensor quenching efficiency, the spectral features of QDs and DOX were carefully studied, and the complex nature of QD fluorescence quenching in the presence of DOX was shown. Using optimized conditions, turn-off fluorescence nanosensors for direct DOX determination in undiluted human plasma were developed. A DOX concentration of 0.5 µM in plasma was reflected in a decrease in the fluorescence intensity of QDs, stabilized with thioglycolic and 3-mercaptopropionic acids, for 5.8 and 4.4 %, respectively. The calculated Limit of Detection values were 0.08 and 0.03 μg/mL using QDs, stabilized with thioglycolic and 3-mercaptopropionic acids, respectively. |
| first_indexed | 2024-03-11T02:41:28Z |
| format | Article |
| id | doaj.art-12c3a6ea500845d58aa92ea7afe4dc7e |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T02:41:28Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-12c3a6ea500845d58aa92ea7afe4dc7e2023-11-18T09:32:29ZengMDPI AGBiosensors2079-63742023-05-0113659610.3390/bios13060596Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin DetectionSvetlana A. Mescheryakova0Ivan S. Matlakhov1Pavel D. Strokin2Daniil D. Drozd3Irina Yu. Goryacheva4Olga A. Goryacheva5Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University Named after N.G. Chernyshevsky, Astrakhanskaya 83, 410012 Saratov, RussiaDepartment of General and Inorganic Chemistry, Chemistry Institute, Saratov State University Named after N.G. Chernyshevsky, Astrakhanskaya 83, 410012 Saratov, RussiaDepartment of General and Inorganic Chemistry, Chemistry Institute, Saratov State University Named after N.G. Chernyshevsky, Astrakhanskaya 83, 410012 Saratov, RussiaDepartment of General and Inorganic Chemistry, Chemistry Institute, Saratov State University Named after N.G. Chernyshevsky, Astrakhanskaya 83, 410012 Saratov, RussiaDepartment of General and Inorganic Chemistry, Chemistry Institute, Saratov State University Named after N.G. Chernyshevsky, Astrakhanskaya 83, 410012 Saratov, RussiaDepartment of General and Inorganic Chemistry, Chemistry Institute, Saratov State University Named after N.G. Chernyshevsky, Astrakhanskaya 83, 410012 Saratov, RussiaDoxorubicin (DOX) is widely used in chemotherapy as an anti-tumor drug. However, DOX is highly cardio-, neuro- and cytotoxic. For this reason, the continuous monitoring of DOX concentrations in biofluids and tissues is important. Most methods for the determination of DOX concentrations are complex and costly, and are designed to determine pure DOX. The purpose of this work is to demonstrate the capabilities of analytical nanosensors based on the quenching of the fluorescence of alloyed CdZnSeS/ZnS quantum dots (QDs) for operative DOX detection. To maximize the nanosensor quenching efficiency, the spectral features of QDs and DOX were carefully studied, and the complex nature of QD fluorescence quenching in the presence of DOX was shown. Using optimized conditions, turn-off fluorescence nanosensors for direct DOX determination in undiluted human plasma were developed. A DOX concentration of 0.5 µM in plasma was reflected in a decrease in the fluorescence intensity of QDs, stabilized with thioglycolic and 3-mercaptopropionic acids, for 5.8 and 4.4 %, respectively. The calculated Limit of Detection values were 0.08 and 0.03 μg/mL using QDs, stabilized with thioglycolic and 3-mercaptopropionic acids, respectively.https://www.mdpi.com/2079-6374/13/6/596quantum dotsdoxorubicinnanosensorfluorescence quenchingStern–Volmer constantsanthracycline antibiotics |
| spellingShingle | Svetlana A. Mescheryakova Ivan S. Matlakhov Pavel D. Strokin Daniil D. Drozd Irina Yu. Goryacheva Olga A. Goryacheva Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin Detection Biosensors quantum dots doxorubicin nanosensor fluorescence quenching Stern–Volmer constants anthracycline antibiotics |
| title | Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin Detection |
| title_full | Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin Detection |
| title_fullStr | Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin Detection |
| title_full_unstemmed | Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin Detection |
| title_short | Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin Detection |
| title_sort | fluorescent alloyed cdznses zns nanosensor for doxorubicin detection |
| topic | quantum dots doxorubicin nanosensor fluorescence quenching Stern–Volmer constants anthracycline antibiotics |
| url | https://www.mdpi.com/2079-6374/13/6/596 |
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