Wound Healing and ndash; A Proteomic Analysis of the Effect of Erythropoietin on Granulation Tissue Isolated from ePTFE Implants
Background: Erythropoietin is a multifunctional cytokine, with recent studies indicating a positive effect on wound healing in models of incisional and burn wounds. Objective: The objective of this study was to investigate the changes in protein expression after daily injections of recombinant huma...
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| Format: | Article |
| Language: | English |
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GESDAV
2014-02-01
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| Series: | Archives of Clinical and Experimental Surgery |
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| Online Access: | http://www.scopemed.org/fulltextpdf.php?mno=32304 |
| _version_ | 1797913306555482112 |
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| author | Bekka Christensen Vibeke Koudahl Bent Honor and eacute; Jens Overgaard Tine Engberg Damsgaard Henrik Vorum |
| author_facet | Bekka Christensen Vibeke Koudahl Bent Honor and eacute; Jens Overgaard Tine Engberg Damsgaard Henrik Vorum |
| author_sort | Bekka Christensen |
| collection | DOAJ |
| description | Background: Erythropoietin is a multifunctional cytokine, with recent studies indicating a positive effect on wound healing in models of incisional and burn wounds.
Objective: The objective of this study was to investigate the changes in protein expression after daily injections of recombinant human erythropoietin in granulation tissue isolated from subcutaneous implants of expanded polytetrafluoroethylene (ePTFE tube).
Methods: ePTFE tubes were implanted subcutaneously in twelve C57bl6 mice. Six of the mice were treated with daily subcutaneous injection of recombinant human erythropoietin of 1000 IU/kg. The remaining six mice, the control group, were treated with injections of 0.9% saline. The implants were removed on day 9 after implantation. The granulation tissue was isolated and analyzed for protein expression using a proteomic approach including two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Some of the results were confirmed with Western Blotting.
Results: We identified 33 differentially expressed proteins in the rhEPO-treated group. Three proteins involved in the glycolytic pathway and mdash; Glyceraldehyde-3-phosphate dehydrogenase, Alpha-enolase, and Triosephosphate isomerase and mdash; were altered in the granulation tissue from the rhEPO-treated group.
Conclusion: Daily injection of recombinant human erythropoietin of 1000 IU/kg alters the protein expression of GAPDH, ENOA and TPIS in granulation tissue from wounds on postoperative day 9. The successful combination of proteomic analysis of wound tissue and the ePTFE wound model could advance our knowledge of the complex healing process. [Arch Clin Exp Surg 2014; 3(1.000): 26-33] |
| first_indexed | 2024-04-10T12:09:51Z |
| format | Article |
| id | doaj.art-12c66207c5c04839af82987cc35ebbb8 |
| institution | Directory Open Access Journal |
| issn | 2146-8133 |
| language | English |
| last_indexed | 2024-04-10T12:09:51Z |
| publishDate | 2014-02-01 |
| publisher | GESDAV |
| record_format | Article |
| series | Archives of Clinical and Experimental Surgery |
| spelling | doaj.art-12c66207c5c04839af82987cc35ebbb82023-02-15T16:16:03ZengGESDAVArchives of Clinical and Experimental Surgery2146-81332014-02-0131263310.5455/aces.2013031909061632304Wound Healing and ndash; A Proteomic Analysis of the Effect of Erythropoietin on Granulation Tissue Isolated from ePTFE ImplantsBekka Christensen0Vibeke Koudahl1Bent Honor and eacute;2Jens Overgaard3Tine Engberg Damsgaard4Henrik Vorum5Department of Plastic Surgery, Aarhus University Hospital., Department of Experimental Clinical Oncology Institute of Clinical Medicine, the Faculty of Health Sciences, University of Aarhus Department of Biomedicine, Aarhus University. Department of Plastic Surgery, Aarhus University Hospital. Department of Experimental Clinical Oncology, Aarhus University Hospital. Department of Ophthalmology, Aalborg Hospital, Aarhus University Hospital.Background: Erythropoietin is a multifunctional cytokine, with recent studies indicating a positive effect on wound healing in models of incisional and burn wounds. Objective: The objective of this study was to investigate the changes in protein expression after daily injections of recombinant human erythropoietin in granulation tissue isolated from subcutaneous implants of expanded polytetrafluoroethylene (ePTFE tube). Methods: ePTFE tubes were implanted subcutaneously in twelve C57bl6 mice. Six of the mice were treated with daily subcutaneous injection of recombinant human erythropoietin of 1000 IU/kg. The remaining six mice, the control group, were treated with injections of 0.9% saline. The implants were removed on day 9 after implantation. The granulation tissue was isolated and analyzed for protein expression using a proteomic approach including two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Some of the results were confirmed with Western Blotting. Results: We identified 33 differentially expressed proteins in the rhEPO-treated group. Three proteins involved in the glycolytic pathway and mdash; Glyceraldehyde-3-phosphate dehydrogenase, Alpha-enolase, and Triosephosphate isomerase and mdash; were altered in the granulation tissue from the rhEPO-treated group. Conclusion: Daily injection of recombinant human erythropoietin of 1000 IU/kg alters the protein expression of GAPDH, ENOA and TPIS in granulation tissue from wounds on postoperative day 9. The successful combination of proteomic analysis of wound tissue and the ePTFE wound model could advance our knowledge of the complex healing process. [Arch Clin Exp Surg 2014; 3(1.000): 26-33]http://www.scopemed.org/fulltextpdf.php?mno=32304Erythropoietingranulation tissueePTFE tubewound healingprotein expressionproteomics |
| spellingShingle | Bekka Christensen Vibeke Koudahl Bent Honor and eacute; Jens Overgaard Tine Engberg Damsgaard Henrik Vorum Wound Healing and ndash; A Proteomic Analysis of the Effect of Erythropoietin on Granulation Tissue Isolated from ePTFE Implants Archives of Clinical and Experimental Surgery Erythropoietin granulation tissue ePTFE tube wound healing protein expression proteomics |
| title | Wound Healing and ndash; A Proteomic Analysis of the Effect of Erythropoietin on Granulation Tissue Isolated from ePTFE Implants |
| title_full | Wound Healing and ndash; A Proteomic Analysis of the Effect of Erythropoietin on Granulation Tissue Isolated from ePTFE Implants |
| title_fullStr | Wound Healing and ndash; A Proteomic Analysis of the Effect of Erythropoietin on Granulation Tissue Isolated from ePTFE Implants |
| title_full_unstemmed | Wound Healing and ndash; A Proteomic Analysis of the Effect of Erythropoietin on Granulation Tissue Isolated from ePTFE Implants |
| title_short | Wound Healing and ndash; A Proteomic Analysis of the Effect of Erythropoietin on Granulation Tissue Isolated from ePTFE Implants |
| title_sort | wound healing and ndash a proteomic analysis of the effect of erythropoietin on granulation tissue isolated from eptfe implants |
| topic | Erythropoietin granulation tissue ePTFE tube wound healing protein expression proteomics |
| url | http://www.scopemed.org/fulltextpdf.php?mno=32304 |
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