A Novel Strain of Aurantiochytrium sp. Strain L3W and Its Characteristics of Biomass and Lipid Production Including Valuable Fatty Acids
Thraustochytrids such as Aurantiochytrium are heterotrophic and produce valuable fatty acids (FAs) and therefore expected as a tool for removal of organic compounds in wastewater and simultaneous production of FAs. The four strains of Aurantiochytrium sp. were isolated from the mangrove leaf samples...
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| Format: | Article |
| Language: | English |
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Japan Society on Water Environment
2021-01-01
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| Series: | Journal of Water and Environment Technology |
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| Online Access: |
https://www.jstage.jst.go.jp/article/jwet/19/1/19_20-087/_pdf
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| _version_ | 1818651642591969280 |
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| author | Satoshi Nakai Asmit Das Yuya Maeda Nurlaili Humaidah Masaki Ohno Wataru Nishijima Takehiko Gotoh Tetsuji Okuda |
| author_facet | Satoshi Nakai Asmit Das Yuya Maeda Nurlaili Humaidah Masaki Ohno Wataru Nishijima Takehiko Gotoh Tetsuji Okuda |
| author_sort | Satoshi Nakai |
| collection | DOAJ |
| description | Thraustochytrids such as Aurantiochytrium are heterotrophic and produce valuable fatty acids (FAs) and therefore expected as a tool for removal of organic compounds in wastewater and simultaneous production of FAs. The four strains of Aurantiochytrium sp. were isolated from the mangrove leaf samples, and among these the strain L3W showed the high specific growth rate of 0.27 1/h at 25°C. In addition, this strain grew at the ranges of temperature from 15°C to 35°C, pH from 3 to 9, and salinity from 0.3 to 70 PSU. Under the optimal condition of 25°C, 30 PSU, and pH7, the strain L3W produced 270 mg/g of FAs including 135 mg/g of docosahexaenoic acid, 11 mg/g of pentadecanoic acid and 9.3 mg/g of margaric acid. Furthermore, the strain L3W produced these FAs at pH3 and pH9. The pH and salinity tolerance of the strain L3W might be advantageous in its application for production of valuable FAs under competition with other microorganisms in unsterile wastewater. |
| first_indexed | 2024-12-17T02:09:22Z |
| format | Article |
| id | doaj.art-12c6c662927245a5b08c58eaf36823fe |
| institution | Directory Open Access Journal |
| issn | 1348-2165 1348-2165 |
| language | English |
| last_indexed | 2024-12-17T02:09:22Z |
| publishDate | 2021-01-01 |
| publisher | Japan Society on Water Environment |
| record_format | Article |
| series | Journal of Water and Environment Technology |
| spelling | doaj.art-12c6c662927245a5b08c58eaf36823fe2022-12-21T22:07:36ZengJapan Society on Water EnvironmentJournal of Water and Environment Technology1348-21651348-21652021-01-01191243410.2965/jwet.20-08710.2965/jwet.20-087A Novel Strain of Aurantiochytrium sp. Strain L3W and Its Characteristics of Biomass and Lipid Production Including Valuable Fatty AcidsSatoshi Nakai0Asmit Das1Yuya Maeda2Nurlaili Humaidah3Masaki Ohno4Wataru Nishijima5Takehiko Gotoh6Tetsuji Okuda7 Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima, Japan Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima, Japan Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima, Japan Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima, Japan Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Science, Niigata, Japan Environmental Research and Management Center, Hiroshima University, Higashi-Hiroshima, Japan Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima, Japan Faculty of Advanced Science and Technology, Ryukoku University, Otsu, Japan Thraustochytrids such as Aurantiochytrium are heterotrophic and produce valuable fatty acids (FAs) and therefore expected as a tool for removal of organic compounds in wastewater and simultaneous production of FAs. The four strains of Aurantiochytrium sp. were isolated from the mangrove leaf samples, and among these the strain L3W showed the high specific growth rate of 0.27 1/h at 25°C. In addition, this strain grew at the ranges of temperature from 15°C to 35°C, pH from 3 to 9, and salinity from 0.3 to 70 PSU. Under the optimal condition of 25°C, 30 PSU, and pH7, the strain L3W produced 270 mg/g of FAs including 135 mg/g of docosahexaenoic acid, 11 mg/g of pentadecanoic acid and 9.3 mg/g of margaric acid. Furthermore, the strain L3W produced these FAs at pH3 and pH9. The pH and salinity tolerance of the strain L3W might be advantageous in its application for production of valuable FAs under competition with other microorganisms in unsterile wastewater. https://www.jstage.jst.go.jp/article/jwet/19/1/19_20-087/_pdf aurantiochytrium sp.ω-3 fatty acidodd-chain fatty acidphysiological characteristic |
| spellingShingle | Satoshi Nakai Asmit Das Yuya Maeda Nurlaili Humaidah Masaki Ohno Wataru Nishijima Takehiko Gotoh Tetsuji Okuda A Novel Strain of Aurantiochytrium sp. Strain L3W and Its Characteristics of Biomass and Lipid Production Including Valuable Fatty Acids Journal of Water and Environment Technology aurantiochytrium sp. ω-3 fatty acid odd-chain fatty acid physiological characteristic |
| title | A Novel Strain of Aurantiochytrium sp. Strain L3W and Its Characteristics of Biomass and Lipid Production Including Valuable Fatty Acids |
| title_full | A Novel Strain of Aurantiochytrium sp. Strain L3W and Its Characteristics of Biomass and Lipid Production Including Valuable Fatty Acids |
| title_fullStr | A Novel Strain of Aurantiochytrium sp. Strain L3W and Its Characteristics of Biomass and Lipid Production Including Valuable Fatty Acids |
| title_full_unstemmed | A Novel Strain of Aurantiochytrium sp. Strain L3W and Its Characteristics of Biomass and Lipid Production Including Valuable Fatty Acids |
| title_short | A Novel Strain of Aurantiochytrium sp. Strain L3W and Its Characteristics of Biomass and Lipid Production Including Valuable Fatty Acids |
| title_sort | novel strain of aurantiochytrium sp strain l3w and its characteristics of biomass and lipid production including valuable fatty acids |
| topic | aurantiochytrium sp. ω-3 fatty acid odd-chain fatty acid physiological characteristic |
| url |
https://www.jstage.jst.go.jp/article/jwet/19/1/19_20-087/_pdf
|
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