Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling
The presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of infl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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South Valley University
2021-09-01
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| Series: | SVU-International Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://svu.journals.ekb.eg/article_193550.html |
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| author | Reham Hassan Khaled A. Nematallah Dina M.W. Shibat El-hamed Samy Abdel-Raouf Fahim Morad Noha Abdelmageed |
| author_facet | Reham Hassan Khaled A. Nematallah Dina M.W. Shibat El-hamed Samy Abdel-Raouf Fahim Morad Noha Abdelmageed |
| author_sort | Reham Hassan |
| collection | DOAJ |
| description | The presence of inflammation is one of the key factors in the onset and progression of various medical
disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to
evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of
inflammation; Xylene-, Carrageenan (CGN)-induced inflammation and acetic acid-induced vascularpermeability in mice were used. We also employed molecular docking approach to verify the
underlying mechanism of anti-inflammatory activity of Vinpocetine. In a current investigation,
Vinpocetine showed anti-inflammatory and antioxidant effects on Xylene- and CGN-induced
inflammation in a dose-dependent manner. Vinpocetine reduced inflammatory edema and restored
antioxidant tissue balance, probably via suppression of IL-1β, IL-6, TNF-α, MDA and MPO activity,
and also through increased non-enzymatic antioxidant (GSH) levels in paw tissue. Histopathological
examination showed that Vinpocetine restored normal skin architecture with significant inhibition of
tissue edema, congestion, and cellular infiltration in CGN-challenged paw. Mechanistically,
Vinpocetine showed downregulation the expression of COX-2 protein, in western blot assessment,
that was associated with significant decrease in the level of prostaglandin E 2 (PGE2) levels; a major
metabolic product of COX-2 enzyme. Interestingly, Silico study showed that Vinpocetine has strong
affinity to COX-2, similar to diclofenac, suggested possible mechanism of downregulation of COX2 expression. Therefore, Vinpocetine showed antioxidant and anti-inflammatory responses might, in
part, due to inhibition of COX-2/PGE 2 pathway. |
| first_indexed | 2024-12-17T10:19:08Z |
| format | Article |
| id | doaj.art-12cd1db3717349d99233f6ed42e6308c |
| institution | Directory Open Access Journal |
| issn | 2535-1826 2535-1877 |
| language | English |
| last_indexed | 2024-12-17T10:19:08Z |
| publishDate | 2021-09-01 |
| publisher | South Valley University |
| record_format | Article |
| series | SVU-International Journal of Veterinary Sciences |
| spelling | doaj.art-12cd1db3717349d99233f6ed42e6308c2022-12-21T21:52:50ZengSouth Valley UniversitySVU-International Journal of Veterinary Sciences2535-18262535-18772021-09-0143516910.21608/svu.2021.70729.1119Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 SignalingReham Hassan0Khaled A. Nematallah1Dina M.W. Shibat El-hamed2Samy Abdel-Raouf Fahim Morad3Noha Abdelmageed4Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt. Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, GermanyDepartment of Pharmacognosy, Faculty of Pharmacy, British University in Egypt (BUE), Cairo, EgyptPoultry Research Department, Animal Health Research Institute (AHRI), Qena branch, Qena, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Sohag University, Sohag, EgyptThe presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of inflammation; Xylene-, Carrageenan (CGN)-induced inflammation and acetic acid-induced vascularpermeability in mice were used. We also employed molecular docking approach to verify the underlying mechanism of anti-inflammatory activity of Vinpocetine. In a current investigation, Vinpocetine showed anti-inflammatory and antioxidant effects on Xylene- and CGN-induced inflammation in a dose-dependent manner. Vinpocetine reduced inflammatory edema and restored antioxidant tissue balance, probably via suppression of IL-1β, IL-6, TNF-α, MDA and MPO activity, and also through increased non-enzymatic antioxidant (GSH) levels in paw tissue. Histopathological examination showed that Vinpocetine restored normal skin architecture with significant inhibition of tissue edema, congestion, and cellular infiltration in CGN-challenged paw. Mechanistically, Vinpocetine showed downregulation the expression of COX-2 protein, in western blot assessment, that was associated with significant decrease in the level of prostaglandin E 2 (PGE2) levels; a major metabolic product of COX-2 enzyme. Interestingly, Silico study showed that Vinpocetine has strong affinity to COX-2, similar to diclofenac, suggested possible mechanism of downregulation of COX2 expression. Therefore, Vinpocetine showed antioxidant and anti-inflammatory responses might, in part, due to inhibition of COX-2/PGE 2 pathway.https://svu.journals.ekb.eg/article_193550.htmlcox-2/pge 2 pathwaycytokinesoxidative stressvinpocetine |
| spellingShingle | Reham Hassan Khaled A. Nematallah Dina M.W. Shibat El-hamed Samy Abdel-Raouf Fahim Morad Noha Abdelmageed Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling SVU-International Journal of Veterinary Sciences cox-2/pge 2 pathway cytokines oxidative stress vinpocetine |
| title | Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling |
| title_full | Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling |
| title_fullStr | Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling |
| title_full_unstemmed | Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling |
| title_short | Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling |
| title_sort | vinpocetine suppresses inflammatory and oxidative machineries in acute model of inflammation pivotal role of cox 2 signaling |
| topic | cox-2/pge 2 pathway cytokines oxidative stress vinpocetine |
| url | https://svu.journals.ekb.eg/article_193550.html |
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