Biodegradation of petroleum by bacteria isolated from fishes of Indian Ocean
Abstract In this study, oil degrading bacteria discovered from fish living near the oil ports at Karachi in Pakistan were characterized. The bacteria isolated from skin, gills, and gut in fish could consume crude oil as a source of carbon and energy. Total 36 isolates were tested using Nutrient Agar...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-06-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100244&tlng=en |
| _version_ | 1798021406079844352 |
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| author | S. Ullah N. Ali F. U. Dawar M. Nughman M. Rauf M. N. K. Khattak B. C. Kim |
| author_facet | S. Ullah N. Ali F. U. Dawar M. Nughman M. Rauf M. N. K. Khattak B. C. Kim |
| author_sort | S. Ullah |
| collection | DOAJ |
| description | Abstract In this study, oil degrading bacteria discovered from fish living near the oil ports at Karachi in Pakistan were characterized. The bacteria isolated from skin, gills, and gut in fish could consume crude oil as a source of carbon and energy. Total 36 isolates were tested using Nutrient Agar (NA) and MSA media with different crude oil concentrations (0.2%, 0.5%, 0.7%, 1%, 2%, and 5%) and 4 out of 36 isolates (two Gram positive and two Gram negative bacteria) were selected for further identification. 16S rRNA gene sequencing revealed that the isolates are related to Bacillus velezensis, Bacillus flexus, Pseudomonas brenneri and Pseudomonas azotoforman. Oil degrading potential of these bacteria was characterized by GC-MS analysis of degradation of oil components in crude oil as well as engine oil. We found that one (2, 6, 10, 14-Tetramethylpentadecane) out of 42 components in the crude oil was fully eliminated and the other oil components were reduced. In addition, 26 out of 42 oil components in the engine oil, were fully eliminated and the rest were amended. Taken together, these studies identify that B. velezensis, B. flexus, P. brenneri and P. azotoforman have high oil degrading potential, which may be useful for degradation of oil pollutants and other commercial applications. |
| first_indexed | 2024-04-11T17:13:20Z |
| format | Article |
| id | doaj.art-12ce162ea36149a98c04568b9079dc62 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-11T17:13:20Z |
| publishDate | 2021-06-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-12ce162ea36149a98c04568b9079dc622022-12-22T04:12:48ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-06-018210.1590/1519-6984.244703Biodegradation of petroleum by bacteria isolated from fishes of Indian OceanS. Ullahhttps://orcid.org/0000-0001-9104-4313N. Alihttps://orcid.org/0000-0002-4651-9946F. U. Dawarhttps://orcid.org/0000-0002-1780-4510M. Nughmanhttps://orcid.org/0000-0003-4539-6478M. Raufhttps://orcid.org/0000-0002-2884-3283M. N. K. Khattakhttps://orcid.org/0000-0002-2286-3451B. C. Kimhttps://orcid.org/0000-0001-5101-4339Abstract In this study, oil degrading bacteria discovered from fish living near the oil ports at Karachi in Pakistan were characterized. The bacteria isolated from skin, gills, and gut in fish could consume crude oil as a source of carbon and energy. Total 36 isolates were tested using Nutrient Agar (NA) and MSA media with different crude oil concentrations (0.2%, 0.5%, 0.7%, 1%, 2%, and 5%) and 4 out of 36 isolates (two Gram positive and two Gram negative bacteria) were selected for further identification. 16S rRNA gene sequencing revealed that the isolates are related to Bacillus velezensis, Bacillus flexus, Pseudomonas brenneri and Pseudomonas azotoforman. Oil degrading potential of these bacteria was characterized by GC-MS analysis of degradation of oil components in crude oil as well as engine oil. We found that one (2, 6, 10, 14-Tetramethylpentadecane) out of 42 components in the crude oil was fully eliminated and the other oil components were reduced. In addition, 26 out of 42 oil components in the engine oil, were fully eliminated and the rest were amended. Taken together, these studies identify that B. velezensis, B. flexus, P. brenneri and P. azotoforman have high oil degrading potential, which may be useful for degradation of oil pollutants and other commercial applications.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100244&tlng=enoil degradationbacteriabioremediationfishIndian Ocean |
| spellingShingle | S. Ullah N. Ali F. U. Dawar M. Nughman M. Rauf M. N. K. Khattak B. C. Kim Biodegradation of petroleum by bacteria isolated from fishes of Indian Ocean Brazilian Journal of Biology oil degradation bacteria bioremediation fish Indian Ocean |
| title | Biodegradation of petroleum by bacteria isolated from fishes of Indian Ocean |
| title_full | Biodegradation of petroleum by bacteria isolated from fishes of Indian Ocean |
| title_fullStr | Biodegradation of petroleum by bacteria isolated from fishes of Indian Ocean |
| title_full_unstemmed | Biodegradation of petroleum by bacteria isolated from fishes of Indian Ocean |
| title_short | Biodegradation of petroleum by bacteria isolated from fishes of Indian Ocean |
| title_sort | biodegradation of petroleum by bacteria isolated from fishes of indian ocean |
| topic | oil degradation bacteria bioremediation fish Indian Ocean |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100244&tlng=en |
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