Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors
While studying c-Myc protein expression in several Burkitt lymphoma cell lines and in lymph nodes from a mouse model bearing a translocated <i>c-MYC</i> gene from the human BL line IARC-BL60, we surprisingly discovered a complex electrophoretic profile. Indeed, the BL60 cell line carryin...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/6/836 |
| _version_ | 1797489509516967936 |
|---|---|
| author | Dina Ibrahim Léa Prévaud Nathalie Faumont Danielle Troutaud Jean Feuillard Mona Diab-Assaf Ahmad Oulmouden |
| author_facet | Dina Ibrahim Léa Prévaud Nathalie Faumont Danielle Troutaud Jean Feuillard Mona Diab-Assaf Ahmad Oulmouden |
| author_sort | Dina Ibrahim |
| collection | DOAJ |
| description | While studying c-Myc protein expression in several Burkitt lymphoma cell lines and in lymph nodes from a mouse model bearing a translocated <i>c-MYC</i> gene from the human BL line IARC-BL60, we surprisingly discovered a complex electrophoretic profile. Indeed, the BL60 cell line carrying the <i>t</i>(8;22) <i>c-MYC</i> translocation exhibits a simple pattern, with a single c-Myc2 isoform. Analysis of the <i>c-MYC</i> transcripts expressed by tumor lymph nodes in the mouse <i>λc-MYC</i> (<i>A<sup>vy</sup>/a</i>) showed for the first time five transcripts that are associated with <i>t</i>(8;22) <i>c-MYC</i> translocation. The five transcripts were correlated with the production of c-Myc2 and c-MycS, and loss of c-Myc1. The contribution of these transcripts to the oncogenic activation of the <i>t</i>(8;22) <i>c-MYC</i> is discussed. |
| first_indexed | 2024-03-10T00:17:37Z |
| format | Article |
| id | doaj.art-12d08cacf6d741b5a3776e63b4121d96 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T00:17:37Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-12d08cacf6d741b5a3776e63b4121d962023-11-23T15:47:48ZengMDPI AGBiomolecules2218-273X2022-06-0112683610.3390/biom12060836Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 TumorsDina Ibrahim0Léa Prévaud1Nathalie Faumont2Danielle Troutaud3Jean Feuillard4Mona Diab-Assaf5Ahmad Oulmouden6CNRS UMR-7276, INSERM U1262, CRIBL, Dupuytren Hospital University Center (CHU) of Limoges, University of Limoges, 87036 Limoges, FranceCNRS UMR-7276, INSERM U1262, CRIBL, Dupuytren Hospital University Center (CHU) of Limoges, University of Limoges, 87036 Limoges, FranceCNRS UMR-7276, INSERM U1262, CRIBL, Dupuytren Hospital University Center (CHU) of Limoges, University of Limoges, 87036 Limoges, FranceCNRS UMR-7276, INSERM U1262, CRIBL, Dupuytren Hospital University Center (CHU) of Limoges, University of Limoges, 87036 Limoges, FranceCNRS UMR-7276, INSERM U1262, CRIBL, Dupuytren Hospital University Center (CHU) of Limoges, University of Limoges, 87036 Limoges, FranceDepartment of Sciences, Lebanese University Fanar, Beirut 1500, LebanonCNRS UMR-7276, INSERM U1262, CRIBL, Dupuytren Hospital University Center (CHU) of Limoges, University of Limoges, 87036 Limoges, FranceWhile studying c-Myc protein expression in several Burkitt lymphoma cell lines and in lymph nodes from a mouse model bearing a translocated <i>c-MYC</i> gene from the human BL line IARC-BL60, we surprisingly discovered a complex electrophoretic profile. Indeed, the BL60 cell line carrying the <i>t</i>(8;22) <i>c-MYC</i> translocation exhibits a simple pattern, with a single c-Myc2 isoform. Analysis of the <i>c-MYC</i> transcripts expressed by tumor lymph nodes in the mouse <i>λc-MYC</i> (<i>A<sup>vy</sup>/a</i>) showed for the first time five transcripts that are associated with <i>t</i>(8;22) <i>c-MYC</i> translocation. The five transcripts were correlated with the production of c-Myc2 and c-MycS, and loss of c-Myc1. The contribution of these transcripts to the oncogenic activation of the <i>t</i>(8;22) <i>c-MYC</i> is discussed.https://www.mdpi.com/2218-273X/12/6/836lymphoma cell lineslymph nodesc-Myc proteinBL60 cell line |
| spellingShingle | Dina Ibrahim Léa Prévaud Nathalie Faumont Danielle Troutaud Jean Feuillard Mona Diab-Assaf Ahmad Oulmouden Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors Biomolecules lymphoma cell lines lymph nodes c-Myc protein BL60 cell line |
| title | Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors |
| title_full | Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors |
| title_fullStr | Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors |
| title_full_unstemmed | Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors |
| title_short | Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors |
| title_sort | alternative i c myc i mrna transcripts as an additional tool for c myc2 and c mycs production in bl60 tumors |
| topic | lymphoma cell lines lymph nodes c-Myc protein BL60 cell line |
| url | https://www.mdpi.com/2218-273X/12/6/836 |
| work_keys_str_mv | AT dinaibrahim alternativeicmycimrnatranscriptsasanadditionaltoolforcmyc2andcmycsproductioninbl60tumors AT leaprevaud alternativeicmycimrnatranscriptsasanadditionaltoolforcmyc2andcmycsproductioninbl60tumors AT nathaliefaumont alternativeicmycimrnatranscriptsasanadditionaltoolforcmyc2andcmycsproductioninbl60tumors AT danielletroutaud alternativeicmycimrnatranscriptsasanadditionaltoolforcmyc2andcmycsproductioninbl60tumors AT jeanfeuillard alternativeicmycimrnatranscriptsasanadditionaltoolforcmyc2andcmycsproductioninbl60tumors AT monadiabassaf alternativeicmycimrnatranscriptsasanadditionaltoolforcmyc2andcmycsproductioninbl60tumors AT ahmadoulmouden alternativeicmycimrnatranscriptsasanadditionaltoolforcmyc2andcmycsproductioninbl60tumors |