The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)
Abstract Plants respond to wounding caused by mechanical stress or herbivory by synthesizing defense proteins. There are no studies reporting the action of induced plant proteins against ticks. The aim of this study was to investigate the effect of mechanically wounded Leucaena leucocephala leaves a...
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Colégio Brasileiro de Parasitologia Veterinaria
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200171&lng=en&tlng=en |
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| author | Lêdia Feitosa Wanderley Karla Lílian Rodrigues Batista Jorgiane Furtado de Carvalho Aldilene da Silva Lima Gabriel Alves Landulfo Alexandra Martins dos Santos Soares Livio Martins Costa Junior |
| author_facet | Lêdia Feitosa Wanderley Karla Lílian Rodrigues Batista Jorgiane Furtado de Carvalho Aldilene da Silva Lima Gabriel Alves Landulfo Alexandra Martins dos Santos Soares Livio Martins Costa Junior |
| author_sort | Lêdia Feitosa Wanderley |
| collection | DOAJ |
| description | Abstract Plants respond to wounding caused by mechanical stress or herbivory by synthesizing defense proteins. There are no studies reporting the action of induced plant proteins against ticks. The aim of this study was to investigate the effect of mechanically wounded Leucaena leucocephala leaves against Rhipicephalus (Boophilus) microplus. Initially, we carried out time course experiments to evaluate the impact of mechanical wounding on the protein content and the peroxidase, catalase and protease inhibitor activities in L. leucocephala. We then evaluated the acaricidal activity on R. (B.) microplus from protein extract collected from L. leucocephala after mechanical wounding. L. leucocephala leaves were artificially wounded, and after 6, 12, 24 and 48h, the leaves were collected for protein extraction. Quantitative and qualitative analyses of the proteins were performed. The protein content and peroxidase and protease activities increased 12h after wounding, and the acaricidal activity of this protein extract was evaluated using engorged R. (B.) microplus females. The protein extract obtained after wounding reduced egg production (8.5%) compared to those without wounding. Furthermore, the extract reduced egg hatching by 47.7% and showed an overall efficacy of 56.3% at 0.1 mgP/mL of the protein. We demonstrated that L. leucocephala defensive proteins could be effective against R. (B.) microplus. |
| first_indexed | 2024-12-10T17:58:05Z |
| format | Article |
| id | doaj.art-12d1d14652bb4de4b50028d546d9bd28 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-10T17:58:05Z |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-12d1d14652bb4de4b50028d546d9bd282022-12-22T01:38:52ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-296126217117610.1590/s1984-29612017026S1984-29612017000200171The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)Lêdia Feitosa WanderleyKarla Lílian Rodrigues BatistaJorgiane Furtado de CarvalhoAldilene da Silva LimaGabriel Alves LandulfoAlexandra Martins dos Santos SoaresLivio Martins Costa JuniorAbstract Plants respond to wounding caused by mechanical stress or herbivory by synthesizing defense proteins. There are no studies reporting the action of induced plant proteins against ticks. The aim of this study was to investigate the effect of mechanically wounded Leucaena leucocephala leaves against Rhipicephalus (Boophilus) microplus. Initially, we carried out time course experiments to evaluate the impact of mechanical wounding on the protein content and the peroxidase, catalase and protease inhibitor activities in L. leucocephala. We then evaluated the acaricidal activity on R. (B.) microplus from protein extract collected from L. leucocephala after mechanical wounding. L. leucocephala leaves were artificially wounded, and after 6, 12, 24 and 48h, the leaves were collected for protein extraction. Quantitative and qualitative analyses of the proteins were performed. The protein content and peroxidase and protease activities increased 12h after wounding, and the acaricidal activity of this protein extract was evaluated using engorged R. (B.) microplus females. The protein extract obtained after wounding reduced egg production (8.5%) compared to those without wounding. Furthermore, the extract reduced egg hatching by 47.7% and showed an overall efficacy of 56.3% at 0.1 mgP/mL of the protein. We demonstrated that L. leucocephala defensive proteins could be effective against R. (B.) microplus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200171&lng=en&tlng=enMechanical stressplant proteininduced defenseacaricidetick |
| spellingShingle | Lêdia Feitosa Wanderley Karla Lílian Rodrigues Batista Jorgiane Furtado de Carvalho Aldilene da Silva Lima Gabriel Alves Landulfo Alexandra Martins dos Santos Soares Livio Martins Costa Junior The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Revista Brasileira de Parasitologia Veterinária Mechanical stress plant protein induced defense acaricide tick |
| title | The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
| title_full | The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
| title_fullStr | The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
| title_full_unstemmed | The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
| title_short | The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) |
| title_sort | first assessment of the stress inducible defense of leucaena leucocephala with acaricidal potential effect against rhipicephalus boophilus microplus acari ixodidae |
| topic | Mechanical stress plant protein induced defense acaricide tick |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200171&lng=en&tlng=en |
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