Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk
ABSTRACT: The demand for commercially available human breast milk has significantly increased in recent years. For various reasons, a significant amount of commercially available human breast milk is being adulterated with other types of milk. This fraudulent practice poses a threat to consumers...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
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| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030223003922 |
| _version_ | 1797741452833325056 |
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| author | Mahmoud K. Eldahshoury Ian P. Hurley |
| author_facet | Mahmoud K. Eldahshoury Ian P. Hurley |
| author_sort | Mahmoud K. Eldahshoury |
| collection | DOAJ |
| description | ABSTRACT: The demand for commercially available human breast milk has significantly increased in recent years. For various reasons, a significant amount of commercially available human breast milk is being adulterated with other types of milk. This fraudulent practice poses a threat to consumers' health due to potential adulterants such as cow milk, which may put the infant at risk due to intolerance or allergy. A direct sandwich anti-bovine IgG ELISA has been developed for the sensitive and specific detection of cow milk in adulterated human breast milk. This assay uses polyclonal anti-bovine IgG antibody as a capture antibody and monoclonal anti-bovine IgG-alkaline phosphatase antibody as a detection antibody. Once optimized, the assay was found to be highly sensitive, and specific to bovine IgG. The assay had no significant cross-reaction with human breast milk, indicating that it was highly specific. The anti-bovine IgG ELISA was able to detect the presence of cow milk in adulterated human breast milk with a detection limit of 0.001% cow milk. The developed assay was highly reproducible (coefficient of variation <10%). The developed direct sandwich anti-bovine IgG ELISA is simple, reliable, and reproducible, making it an ideal test for this purpose. |
| first_indexed | 2024-03-12T14:26:57Z |
| format | Article |
| id | doaj.art-12d300bef6924ad3be58ed93a18563b9 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-03-12T14:26:57Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-12d300bef6924ad3be58ed93a18563b92023-08-18T04:30:23ZengElsevierJournal of Dairy Science0022-03022023-09-01106959085915Direct sandwich ELISA to detect the adulteration of human breast milk by cow milkMahmoud K. Eldahshoury0Ian P. Hurley1School of Health, Leeds Beckett University, Leeds LS13HE, United KingdomCorresponding author; School of Health, Leeds Beckett University, Leeds LS13HE, United KingdomABSTRACT: The demand for commercially available human breast milk has significantly increased in recent years. For various reasons, a significant amount of commercially available human breast milk is being adulterated with other types of milk. This fraudulent practice poses a threat to consumers' health due to potential adulterants such as cow milk, which may put the infant at risk due to intolerance or allergy. A direct sandwich anti-bovine IgG ELISA has been developed for the sensitive and specific detection of cow milk in adulterated human breast milk. This assay uses polyclonal anti-bovine IgG antibody as a capture antibody and monoclonal anti-bovine IgG-alkaline phosphatase antibody as a detection antibody. Once optimized, the assay was found to be highly sensitive, and specific to bovine IgG. The assay had no significant cross-reaction with human breast milk, indicating that it was highly specific. The anti-bovine IgG ELISA was able to detect the presence of cow milk in adulterated human breast milk with a detection limit of 0.001% cow milk. The developed assay was highly reproducible (coefficient of variation <10%). The developed direct sandwich anti-bovine IgG ELISA is simple, reliable, and reproducible, making it an ideal test for this purpose.http://www.sciencedirect.com/science/article/pii/S0022030223003922human breast milk adulterationsandwich ELISAIgGfood adulteration |
| spellingShingle | Mahmoud K. Eldahshoury Ian P. Hurley Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk Journal of Dairy Science human breast milk adulteration sandwich ELISA IgG food adulteration |
| title | Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk |
| title_full | Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk |
| title_fullStr | Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk |
| title_full_unstemmed | Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk |
| title_short | Direct sandwich ELISA to detect the adulteration of human breast milk by cow milk |
| title_sort | direct sandwich elisa to detect the adulteration of human breast milk by cow milk |
| topic | human breast milk adulteration sandwich ELISA IgG food adulteration |
| url | http://www.sciencedirect.com/science/article/pii/S0022030223003922 |
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